At-line Analysis for Cell Culture Media
REBEL
At-line cell culture media analysis. Results on 30+ components in under 10 minutes. When and where is your call. With the REBEL, now is always on the table.
Easy to understand answers
No more waiting for third party testing
Accurate, reliable performance whether running 1 or 100 samples
MAVERICK, REBEL, MAVEN, and ZipChip have joined the Repligen product family!
These solutions will continue to be available and supported by Repligen.Â
Waiting weeks for cell culture media analysis results is a thing of the past. With REBEL’s at-line analysis capabilities, you can assess over 30 media components in less than 10 minutes. Instead of sending samples to the lab, get the data where and when you need it.
Cell Culture Media Matters. Here’s Why.
From ensuring your media contains the right analytes to faster media and process development, hassle-free cell culture media analysis using REBEL enables accelerated time to market while lowering cost and gaining process insights.
Fresh Media Fingerprinting
Save money by avoiding risk of batch failure with fresh media formulation analysis.
Media Development
Know your cell nutrient requirements and accelerate media development timelines.
Process Development
Reduce process development timelines and improve productivity.
Process Monitoring
Get to the root cause of manufacturing process variations and decrease risk of wasted batches.
Process Modeling
Add the missing pieces to your predictive model for smarter DOE resulting in faster process development.
Reduce Downtime Between Experiments & Save Money
Fewer Bioreactor Runs, Less FTE Time, Shorter Development Time
Enhance your bioprocess and improve efficiency of process development workflows with the REBEL, at-line cell culture media analyzer. The device allows you to monitor concentration changes of amino acids, vitamins, and amines in the media throughout the entire bioreactor run. This essential data enables you to correlate changes in the media to product quality and yield for optimizing and refining feeding strategies, enabling maximum cell growth and productivity. REBEL empowers scientists to make informed decisions that can significantly impact biotherapeutic development.
Traditional Workflow With Core or External Lab Analytics:
REBEL Assisted Workflow:
*REBEL used in combination with modeling can lead to fewer bioreactor runs.
New Software Increases Usability and Efficiency​
Our latest REBEL software release has powerful new features and enhancements that optimize system performance and improve usability and operational efficiency. Improvements in system handling enable smoother operations and increased system longevity. System performance enhancements enable a more efficient startup process, reductions in system idle time, and automates warm-up after a long-term shutdown. Usability and efficiency improvements include better workflow transparency of consumables’ lifetime and enabling user-level account access to essential functions.
All current REBEL users are encouraged to update their device to version 1.4.2 software. More information and download instructions are available in our Customer Support Portal. If you’re considering purchasing a REBEL device, contact us today to learn more about how REBEL can help you to gain insights on your cell culture process and accelerate workflows.
Trusted by BioPharma​
Listen to how scientists have utilized REBEL to speed up their process development. Gain timely insights into metabolic shifts of cell culture. Quickly optimize cell culture media and feeds.
Application Spotlight
Keeping Up with New Emerging Technology
Processing and analysis of bioreactor samples for critical process parameters represents a key bottleneck at multiple stages of biotherapeutic drug development, including pre-clinical optimization studies through commercial manufacturing and monitoring. Traditional sample analysis methods such as liquid chromatography-mass spectrometry (LC-MS) require significant technical expertise and time-consuming method development.
Scientists from the U.S. Food and Drug Administration observed that REBEL, a novel, dedicated capillary electrophoresis-mass spectrometry (CE-MS) device, provides a similar ability compared to traditional LC-MS to specifically identify and quantitate an array of amino acids in spent media analysis, enabling user-friendly at-line collection of quantitative data without requiring significant method development or time-consuming data processing.
Learn more about modern analytical technologies for bioprocessing in the webinar on demand or article summary, specifically how to:
- Ascertain the capabilities and limitations of the Amino Acid Analyzer.
- Compare the Amino Acid Analyzer, a new generation of CE-MS analytical platform, against traditional LC-MS methods.
- Evaluate the data quality, trade-offs in operation, and resourcefulness of the analytical platforms.
What If You Could Reduce Analytics from 3-6 Weeks to 10 Minutes?
Traditional Analytics: Can Take 3 to 6 Weeks
The traditional analytics process can take 3–6 weeks, which can lead to prolonged decision-making.
STEP ONE
Pull sample
STEP TWO
Filter cells
STEP THREE
Label, secure, collect, freeze samples (14 days), and fill out paperwork
STEP FOUR
Send frozen batch to core lab
STEP FIVE
Core lab defrosts, dilutes, preps derivatization reagents
STEP SIX
Core lab mixes samples with chemicals
STEP SEVEN
Reacted samples put into HPLC
STEP EIGHT
Run HPLC, acquire data
STEP NINE
Analyze data, summarize results
Report Generated
REBEL Analytics: Only Takes 10 Minutes
The REBEL provides analytics within 10 minutes, enabling quick decision-making.
STEP ONE
Pull sample
STEP TWO
Spin out cells and dilute
STEP THREE
Insert sample into REBEL
STEP FOUR
Start REBEL
Report Generated!
Empower Your Lab with the REBEL Amino Acid Analyzer for Cell Culture Media
Getting precise and accurate results is fast and easy. Optimize cell culture processes with the REBEL analyzer. To start, simply extract a bioreactor sample, spin it down, and dilute. Load up to 96 samples into REBEL and start analysis. The system is designed with ease of use in mind, requiring no prior expertise to run the analysis.
REBEL delivers comprehensive cell culture media composition reports automatically, all of which are directly accessible on your network drive. REBEL’s ease of use and small footprint makes it suitable for labs of all sizes. Add REBEL to your lab’s analytical toolbox to enhance you cell culture media and feed development and optimization workflows. It is more than just an analyzer; it is a pivotal tool that empowers process development teams to stay ahead, recognized as one of the top transformational laboratory technologies by the analytical scientist magazine in 2019.
Significantly accelerate process development cycles, enhance process understanding, and facilitate a more dynamic approach to cell culture optimization with REBEL.
Download Sample Report:
Remove Data Analysis Bottlenecks
REBEL users with access to the JMP statistical software package are able to expedite high-throughput data analysis by REBEL data visualization. REBEL users can download the add-in tool from the 908 Devices customer support portal. To learn more about the REBEL and the visualization tool, watch the BPI: Ask the Expert Webinar, and click the button to obtain a JMP software trial.
REBEL Data Processing
Quantitative numbers for each analyte, from each sample directly from the REBEL.
JMP Statistical Discovery
Expedite high-throughput data analysis from REBEL with JMP statistical discovery software.
Consumables That Don’t Consume Your Time
Easy Sample
Preparation
Just filter, spin, and dilute your samples. There’s no chemical reaction, and it works for both low and high sample throughput needs
Low-Effort Method Development
Use the touch screen to get the sample running. BGE carries the samples through the microchip for ESI-MS detection
Simple System
Check
The REBEL makes it easy with a built-in quantification calibration and system performance check with REBEL SMA standard
Comprehensive
Analysis
30+ analytes including amino acids, vitamins, and amines are quantified in as little as 10 minutes per sample
How Will You Use REBEL?
Monitor amino acids and media composition over the course of the cell culture process. Identify depleted nutrients and refine supplementation strategies on the fly. Correlate media composition to product yield and quality.
Decoding Amino Acids, Biogenic Amines, and Vitamins with REBEL
REBEL offers a groundbreaking approach to the once insurmountable challenge of analyzing the intricacies of cell culture environments. Gain an unprecedented view into a wide spectrum of critical components in cell culture media, from amino acids to biogenic amines to essential vitamins. Each of these play a unique role in cellular function and metabolism, and REBEL is your key to streamlining your research workflows.
Amino Acids
- Alanine
- Alanyl-Glutamine
- Arginine
- Asparagine
- Aspartic acid
- Betaine
- Cystine
- Glutamic acid
- Glutamine
- Glycine
- Histidine
- Isoleucine
- Leucine
- Lysine
- Methionine
- Phenylalanine
- Proline
- Serine
- Threonine
- Tryptophan
- Tyrosine
- Valine
Vitamins
- Choline
- Nicotinamide
- Pyridoxal
- Pyridoxine
- Thiamine
Biogenic Amines
- GABA
- Beta-Alanine
- Hydroxyproline
- Methyl-L-histidine
- L-Citrulline
Featured Resources
REBEL Brochure
The REBEL is a novel, dedicated cell culture media analyzer designed for rapid, easy, and efficient amino acid and nutrient analysis.
REBEL Specifications Sheet
Get quantitative identification and analysis on a panel of >30 analytes including key amino acids, biogenic amines, and water soluble vitamins.
REBEL Product Video
Be a REBEL with Your Cell Media Analytics
Customer Support Portal
The My 908 portal gives scientists quick access to important resources like the latest software upgrades, product guides, and training materials.
Frequently Asked Questions
What does REBEL do?
REBEL is used for rapid at-line analysis of cell culture media components including 22 amino acids, 5 vitamins, and 5 biogenic amines. Amino acids are critical for cellular metabolism and are often present in media used for expansion of mammalian, microbial, and insect cell lines such as T cells, mesenchymal stem cells (MSCs), CHO, HEK 293, E-coli, Sf9, Sf21, and others.
Why should I choose REBEL?
With REBEL you can quickly and easily quantify concentrations of key nutrients in fresh and spent cell culture media. Its small footprint and award-winning design lets you conveniently analyze media samples on the bench, next to the bioreactors, without leaving your lab. This pioneering tool is powered by the advanced CE-MS (Capillary Electrophoresis – Mass Spectrometry) technology developed by 908 Devices and produces easy-to-understand reports with concentrations of over 30 media components in less than 10 minutes. There is minimal sample prep and no prior expertise is required to operate the device.
How does the REBEL fit alongside my current workflows?
The REBEL cell culture media analyzer is pivotal in accelerating process development, and supports Continuous Process Verification (CPV). Whether your work involves development or manufacturing of therapeutic recombinant proteins (e.g. monoclonal antibodies, bi-specific antibodies, antibody drug conjugates, biosimilars), cell and gene therapies, or cultivated meats, the REBEL can help streamline your cell culture media analysis workflows. By implementing REBEL at your lab, you will eliminate the need for sending out media samples to an external lab and having to wait weeks for results. Instead, you will be able to perform cell culture media analysis, in minutes, as often as you’d like, cost-effectively, with minimal training and no method development.
Having media composition data at your fingertips, helps you shave weeks off the process development time, better optimize your cell culture or fermentation processes, and monitor production runs.
How can REBEL advance cell therapy process development?
REBEL helps advance cell therapy process development with on-demand analysis of cell culture media, right at the bench. REBEL allows you unprecedented insight into variations in amino acid and nutrient concentrations as your cells grow.
How do I load samples and queue up a run on the REBEL?
REBEL accepts both septa-capped vials and 96-well plates (two 96-well plates are provided with each REBEL kit). The samples and standards are kept at 5 °C inside the REBEL’s autosampler and there are plate covers included in the kits to prevent evaporation. Once your samples are loaded, you can either manually enter your sample information using the REBEL’s touchscreen or import the information using the pre-filled Batch Runsheet Template file (downloadable from the Customer Support Portal).
What data formats does REBEL export, and how are the files saved?
Analysis reports are saved in either CSV or PDF file formats to REBEL’s internal memory or user-specified location on the network. We highly recommend exporting the analysis results/reports to a network location to ensure data integrity and prevent loss of data from overwriting when internal device memory gets full.
How many samples can each kit run?
Each REBEL kit can be used to analyze up to 200 samples.
What is the sample volume required for analysis?
The media sample volume is typically between 10-100 uL depending on the sample container (2ml autosampler vials or 96 well plates). Please note that a minimum 10x sample dilution is required.
Can cell culture media samples be run at 908 Devices?
Yes, cell culture media samples can be run at 908 Devices. Samples are chilled at 5° C. Ensure sample cooling tray cover is securely in place and that all vials are capped (or well plate covered with foil adhesive cover) immediately after preparation.
What is the dynamic range for the analytes?
The dynamic range for most analytes is 5-100 uM.
How long does it take to run one sample?
If a PQ has already been run, it would take 10 minutes for a single sample run and 4 minutes for subsequent replicates.
Is REBEL 21 CFR part 11 compliant? How does it handle audit logs?
Yes, REBEL is 21 CFR part 11 complaint. REBEL records and timestamps all the critical system events (i.e., user logins/logouts, password changes, system modifications, completion of assays, maintenance, and performance qualifications). The audit log files are automatically exported to the system’s configured network location. Secondary policies/procedures should be implemented to ensure the integrity of the audit log files once they have been exported to a location on your network.
I’m tight on benchtop space. Can I run the REBEL on a cart?
You can place and run the REBEL on a sturdy cart but please make sure that the cart can support REBEL’s weight and footprint. Ideally there should be a power outlet and a network port nearby to avoid any trip hazards. If you would like to make your REBEL mobile, reach out to us and we will point you in the right direction.
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