Cell Culture Media Analyzer

REBEL

At-line cell culture media analysis. Results on 30+ components in under 10 minutes. When and where is your call. With the REBEL, now is always on the table.

Simple:
Easy to understand answers
Actionable:
No more waiting for third party testing
Reproducible:
Accurate, reliable performance whether running 1 or 100 samples

Waiting weeks for cell culture media analysis results is a thing of the past. With REBEL’s at-line analysis capabilities, you can assess over 30 media components in less than 10 minutes. Instead of sending samples to the lab, get the data where and when you need it.

Reduce Downtime Between Experiments & Save Money

Cost Savings: Fewer bioreactor runs,  Less FTE time, Shorter development time.

Traditional Workflow With Core or External Lab Analytics:
vs

REBEL Assisted Workflow:

*REBEL used in combination with modeling can lead to fewer bioreactor runs.

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Trusted by BioPharma

Listen to how scientists have utilized REBEL to speed up their process development. Gain timely insights into metabolic shifts of cell culture. Quickly optimize cell culture media and feeds.

What If You Could Reduce Analytics from 3-6 Weeks to 10 Minutes?

The traditional analytics process can take 3–6 weeks, which can lead to prolonged decision-making.

Pull sample

Filter cells

Label, secure, collect, freeze samples (14 days), and fill out paperwork

Send frozen batch to core lab

Core lab defrosts, dilutes, preps derivatization reagents

Core lab mixes samples with chemicals

Reacted samples put into HPLC

Run HPLC, acquire data

Analyze data, summarize results


The REBEL provides analytics within 10 minutes, enabling quick decision-making.

Pull sample

Spin out cells and dilute

Insert sample into REBEL

Start REBEL

Empower Your Lab with the REBEL Amino Acid Analyzer for Cell Culture Media

Getting precise and accurate results is fast and easy. Optimize cell culture processes with the REBEL analyzer. To start, simply extract a bioreactor sample, spin it down, and dilute. Load up to 96 samples into REBEL and start analysis. The system is designed with ease of use in mind, requiring no prior expertise to run the analysis.

REBEL delivers comprehensive cell culture media composition reports automatically, all of which are directly accessible on your network drive. REBEL’s ease of use and small footprint makes it suitable for labs of all sizes. Add REBEL to your lab’s analytical toolbox to enhance you cell culture media and feed development and optimization workflows. It is more than just an analyzer; it is a pivotal tool that empowers process development teams to stay ahead, recognized as one of the top transformational laboratory technologies by the analytical scientist magazine in 2019.

Significantly accelerate process development cycles, enhance process understanding, and facilitate a more dynamic approach to cell culture optimization with REBEL.

Remove Data Analysis Bottlenecks

REBEL users with access to the JMP statistical software package are able to expedite high-throughput data analysis by REBEL data visualization. REBEL users can download the add-in tool from the 908 Devices customer support portal. To learn more about the REBEL and the visualization tool, watch the BPI: Ask the Expert Webinar, and click the button to obtain a JMP software trial.

Quantitative numbers for each analyte, from each sample directly from the REBEL.

Expedite high-throughput data analysis from REBEL with JMP statistical discovery software.

Consumables That Don’t Consume Your Time

Easy Sample
Preparation

Just filter, spin, and dilute your samples. There’s no chemical reaction, and it works for both low and high sample throughput needs

Low-Effort Method Development

Use the touch screen to get the sample running. BGE carries the samples through the microchip for ESI-MS detection

Simple System
Check

The REBEL makes it easy with a built-in quantification calibration and system performance check with REBEL SMA standard

Comprehensive
Analysis

30+ analytes including amino acids, vitamins, and amines are quantified in as little as 10 minutes per sample

How Will You Use REBEL?

Monitor amino acids and media composition over the course of the cell culture process. Identify depleted nutrients and refine supplementation strategies on the fly. Correlate media composition to product yield and quality.

Decoding Amino Acids, Biogenic Amines, and Vitamins with REBEL

REBEL offers a groundbreaking approach to the once insurmountable challenge of analyzing the intricacies of cell culture environments. Gain an unprecedented view into a wide spectrum of critical components in cell culture media, from amino acids to biogenic amines to essential vitamins. Each of these play a unique role in cellular function and metabolism, and REBEL is your key to streamlining your research workflows.

Amino Acids

  • Alanine
  • Alanyl-Glutamine
  • Arginine
  • Asparagine
  • Aspartic acid
  • Betaine
  • Cystine
  • Glutamic acid
  • Glutamine
  • Glycine
  • Histidine
  • Isoleucine
  • Leucine
  • Lysine
  • Methionine
  • Phenylalanine
  • Proline
  • Serine
  • Threonine
  • Tryptophan
  • Tyrosine
  • Valine
  • Choline
  • Nicotinamide
  • Pyridoxal
  • Pyridoxine
  • Thiamine
  • GABA
  • Beta-Alanine
  • Hydroxyproline
  • Methyl-L-histidine
  • L-Citrulline

Customer Support Portal

The My 908 portal gives scientists quick access to important resources like the latest software upgrades, product guides, and training materials.

Frequently Asked Questions

What does REBEL do? 

REBEL is used for rapid at-line analysis of cell culture media components including 22 amino acids, 5 vitamins, and 5 biogenic amines. Amino acids are critical for cellular metabolism and are often present in media used for expansion of mammalian, microbial, and insect cell lines such as T cells, mesenchymal stem cells (MSCs), CHO, HEK 293, E-coli, Sf9, Sf21, and others.

Why should I choose REBEL?

With REBEL you can quickly and easily quantify concentrations of key nutrients in fresh and spent cell culture media. Its small footprint and award-winning design lets you conveniently analyze media samples on the bench, next to the bioreactors, without leaving your lab. This pioneering tool is powered by the advanced CE-MS (Capillary Electrophoresis – Mass Spectrometry) technology developed by 908 Devices and produces easy-to-understand reports with concentrations of over 30 media components in less than 10 minutes. There is minimal sample prep and no prior expertise is required to operate the device.

How does the REBEL fit alongside my current workflows?

The REBEL cell culture media analyzer is pivotal in accelerating process development, and supports Continuous Process Verification (CPV). Whether your work involves development or manufacturing of therapeutic recombinant proteins (e.g. monoclonal antibodies, bi-specific antibodies, antibody drug conjugates, biosimilars), cell and gene therapies, or cultivated meats, the REBEL can help streamline your cell culture media analysis workflows. By implementing REBEL at your lab, you will eliminate the need for sending out media samples to an external lab and having to wait weeks for results. Instead, you will be able to perform cell culture media analysis, in minutes, as often as you’d like, cost-effectively, with minimal training and no method development.  

Having media composition data at your fingertips, helps you shave weeks off the process development time, better optimize your cell culture or fermentation processes, and monitor production runs.   

How can REBEL advance cell therapy process development?

REBEL helps advance cell therapy process development with on-demand analysis of cell culture media, right at the bench. REBEL allows you unprecedented insight into variations in amino acid and nutrient concentrations as your cells grow. 

How do I load samples and queue up a run on the REBEL?

REBEL accepts both septa-capped vials and 96-well plates (two 96-well plates are provided with each REBEL kit). The samples and standards are kept at 5 °C inside the REBEL’s autosampler and there are plate covers included in the kits to prevent evaporation. Once your samples are loaded, you can either manually enter your sample information using the REBEL’s touchscreen or import the information using the pre-filled Batch Runsheet Template file (downloadable from the Customer Support Portal).

What data formats does REBEL export, and how are the files saved?

Analysis reports are saved in either CSV or PDF file formats to REBEL’s internal memory or user-specified location on the network. We highly recommend exporting the analysis results/reports to a network location to ensure data integrity and prevent loss of data from overwriting when internal device memory gets full.

How many samples can each kit run? 

Each REBEL kit can be used to analyze up to 200 samples. 

What is the sample volume required for analysis? 

The media sample volume is typically between 10-100 uL depending on the sample container (2ml autosampler vials or 96 well plates).  Please note that a minimum 10x sample dilution is required.

Can cell culture media samples be run at 908 Devices?

Yes, cell culture media samples can be run at 908 Devices. Samples are chilled at 5° C. Ensure sample cooling tray cover is securely in place and that all vials are capped (or well plate covered with foil adhesive cover) immediately after preparation. 

What is the dynamic range for the analytes?

The dynamic range for most analytes is 5-100 uM.

How long does it take to run one sample?

If a PQ has already been run, it would take 10 minutes for a single sample run and 4 minutes for subsequent replicates. 

Is REBEL 21 CFR part 11 compliant? How does it handle audit logs? 

Yes, REBEL is 21 CFR part 11 complaint. REBEL records and timestamps all the critical system events (i.e., user logins/logouts, password changes, system modifications, completion of assays, maintenance, and performance qualifications). The audit log files are automatically exported to the system’s configured network location. Secondary policies/procedures should be implemented to ensure the integrity of the audit log files once they have been exported to a location on your network. 

I’m tight on benchtop space. Can I run the REBEL on a cart?

You can place and run the REBEL on a sturdy cart but please make sure that the cart can support REBEL’s weight and footprint. Ideally there should be a power outlet and a network port nearby to avoid any trip hazards. If you would like to make your REBEL mobile, reach out to us and we will point you in the right direction. 

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