REBEL is used for rapid at-line analysis of cell culture media components including 22 amino acids, 5 vitamins, and 5 biogenic amines. Amino acids are critical for cellular metabolism and are often present in media used for expansion of mammalian, microbial, and insect cell lines such as T cells, mesenchymal stem cells (MSCs), CHO, HEK 293, E-coli, Sf9, Sf21, and others.

With REBEL you can quickly and easily quantify concentrations of key nutrients in fresh and spent cell culture media. Its small footprint and award-winning design lets you conveniently analyze media samples on the bench, next to the bioreactors, without leaving your lab. This pioneering tool is powered by the advanced CE-MS (Capillary Electrophoresis – Mass Spectrometry) technology developed by 908 Devices and produces easy-to-understand reports with concentrations of over 30 media components in less than 10 minutes. There is minimal sample prep and no prior expertise is required to operate the device.

The REBEL cell culture media analyzer is pivotal in accelerating process development, and supports Continuous Process Verification (CPV). Whether your work involves development or manufacturing of therapeutic recombinant proteins (e.g. monoclonal antibodies, bi-specific antibodies, antibody drug conjugates, biosimilars), cell and gene therapies, or cultivated meats, the REBEL can help streamline your cell culture media analysis workflows. By implementing REBEL at your lab, you will eliminate the need for sending out media samples to an external lab and having to wait weeks for results. Instead, you will be able to perform cell culture media analysis, in minutes, as often as you’d like, cost-effectively, with minimal training and no method development.  

Having media composition data at your fingertips, helps you shave weeks off the process development time, better optimize your cell culture or fermentation processes, and monitor production runs.   

REBEL helps advance cell therapy process development with on-demand analysis of cell culture media, right at the bench. REBEL allows you unprecedented insight into variations in amino acid and nutrient concentrations as your cells grow. 

REBEL accepts both septa-capped vials and 96-well plates (two 96-well plates are provided with each REBEL kit). The samples and standards are kept at 5 °C inside the REBEL’s autosampler and there are plate covers included in the kits to prevent evaporation. Once your samples are loaded, you can either manually enter your sample information using the REBEL’s touchscreen or import the information using the pre-filled Batch Runsheet Template file (downloadable from the Customer Support Portal).

Analysis reports are saved in either CSV or PDF file formats to REBEL’s internal memory or user-specified location on the network. We highly recommend exporting the analysis results/reports to a network location to ensure data integrity and prevent loss of data from overwriting when internal device memory gets full.

Each REBEL kit can be used to analyze up to 200 samples. 

The media sample volume is typically between 10-100 uL depending on the sample container (2ml autosampler vials or 96 well plates).  Please note that a minimum 10x sample dilution is required.

Yes, cell culture media samples can be run at 908 Devices. Samples are chilled at 5° C. Ensure sample cooling tray cover is securely in place and that all vials are capped (or well plate covered with foil adhesive cover) immediately after preparation. 

The dynamic range for most analytes is 5-100 uM.

If a PQ has already been run, it would take 10 minutes for a single sample run and 4 minutes for subsequent replicates. 

Yes, REBEL is 21 CFR part 11 complaint. REBEL records and timestamps all the critical system events (i.e., user logins/logouts, password changes, system modifications, completion of assays, maintenance, and performance qualifications). The audit log files are automatically exported to the system’s configured network location. Secondary policies/procedures should be implemented to ensure the integrity of the audit log files once they have been exported to a location on your network. 

You can place and run the REBEL on a sturdy cart but please make sure that the cart can support REBEL’s weight and footprint. Ideally there should be a power outlet and a network port nearby to avoid any trip hazards. If you would like to make your REBEL mobile, reach out to us and we will point you in the right direction.