Application Notes & Snapshots

Application Snapshot Booklet Fermentation

Application Snapshot Booklet Cell and Gene Therapy

Application Snapshot Booklet Cell Culture

Application Snapshot Booklet Biologics

Application Snapshot Booklet

Download our full collection of application snapshots, with focuses on biologics, CGT, and fermentation.

How comparable are different vendors’ LB media?

High variations of analyte levels in Terrific broth (TB) media

Investigating the high nutrient variations in Super Optimal Broth

Cultures’ growth rates and productivities may differ when different vendor’s media are used in otherwise identical experiments.

Probing formulation differences in Super Optimal Broth

Similarities between different vendors’ 2X YT liquid media

Comparing standard microbial media from a single supplier

At-line spent media analysis is now a reality

To elevate process development decisions to real-time, researchers require new analytical platforms that simplify analysis and accelerate delivery of actionable intelligence.

Differences in fresh commercially available hybridoma media

Select the best CHO media from the start

Performing your own quick assays of the media you receive ensures consistency in the cell media fed to your cells.

Screening of chemically-defined media for suspension cultures of HEK293 cells

Across four vendors, there was a high variation in the fresh media composition.

Analyze media without removing your mAb on the Rebel

Identify variations in dry and liquid CHO media

Running a quick fresh media assay prior to feeding the culture ensures consistency of the media components when scaling up or during the transition between liquid-to-dry media blends.

Serum supplemented media analysis is routine with the Rebel

Tease out variations in dry cell culture media

Running a simple media assay prior to feeding your cells can ensure that the composition of the media is what is expected.

Supplementing RPMI media with fetal bovine serum

Supplementing IMDM media with fetal bovine serum

Conventional and chemically-defined T cell medias

Amino acid contents of Sf9 and Sf21 insect cell media

Knowledge of the insect cell metabolism coupled with an understanding of the amino acid content of the cell media allows for a more informed process development analytics strategy.

Comparing mesenchymal stem cell media with supplements on the Rebel

To ensure consistency when culturing MSCs, researchers should understand what is being added to their media.

Don’t sweat your cell media additives

Handle non-ionic surfactants in cell media samples with ease

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Basal cell media with 10% fetal bovine serum

Posters

Optimization of Scalable rAAV Production for Gene Therapy

This poster showcases spent media analysis leveraging REBEL, which requires only 10 μL per sample and 10 minutes analysis time.

Empowering Process Optimization for a mAb-Expressing CHO Cell Line

Coupling miniature mass spectrometry (MS) with microchip capillary electrophoresis (CE) fulfills an unmet need: simple, rapid at-line nutrient quantitation.

Using CE-HPMS For Accelerated Process Development of a mAb-Expressing Cell Line

The coupling of miniature mass spectrometry (MS) to a microchip capillary electrophoresis (CE) platform provides a simple solution for at-line amino acid quantitation.

Dynamic Range Optimization for Amino Acid Quantitation with an Integrated CE-MS Analyzer

The coupling of miniature mass spectrometry (MS) to a microchip capillary electrophoresis (CE) platform provides a simple solution for amino acid quantitation.

Poster-BPI-West-2022

Rapid at line quantitation of key nutrients in cell culture media

This poster details the benefits of coupling of miniature mass spectrometry to a microchip capillary electrophoresis platform for characterizing cell media metabolites in fresh and spent media.

Quantitation of metabolites in classical and customized cell culture media by an integrated CE-MS Analyzer

Quantitation of metabolites in cell culture media by an integrated CE-MS analyzer for upstream process development

Using genomic and spent media analysis to characterize stressed CHO cultures

A Rapid Microfluidic Method for Molecular Weight Determination and Spent Media Analysis of an IgG1 Intact Protein in Growth Media

Characterizing the Analytical Performance and Reliability of Metabolite Quantitation by an Integrated CE-MS Analyzer

Rapid Analysis of Cell Therapy Media

Quantitation of CHO media nutrients and metabolites in under 180 seconds

Rapid analysis of amino acids, water soluble vitamins, biogenic amines and dipeptides in cell growth media

Rapid at line nutrient profiling from an ammonia stressed CHO cell line

Webinars

Multi-Component At-Line Cell Culture Media Analysis

In this talk, Graziella Piras discusses a data-driven and accelerated media screening, selection, and optimization experiment leveraging REBEL.

BPI Europe 2022 - Power Up Your Process Control

Power up your process control with rapid at line MS-based key nutrient monitoring

Graziella Piras, bioprocess segment director for 908 Devices, spoke at BPI Europe 2022 on the benefits of at-line nutrient monitoring.

The Critical Role of Cell Culture Media Analysis in CGT Development

Does cell culture analysis matter for CGT development? This short webinar explores what is already known, reviews at-line methods for cell culture media analysis, and shares recent data from two case studies.

Biopharma 4.0: Finding Opportunities to Leverage New At-Line Data

Listen how REBEL users describe how they use better data acquisition, management platforms, and process modeling to implement process intensification.

Process Modeling with Researchers from Johns Hopkins University

Listen to a dynamic discussion on the highlights of a new peer-reviewed article exploring model-based approached to cell culture optimization

Ask the Expert: Bioprocess 101 – Cell Culture Media Analysis

Process Intensification: Do You Have All the Data You Need When You Need It?

The need for process intensification and implementation of Bioprocess 4.0 requires extensive real time data acquisition and process modelling. At line MS-based technologies presented here provide key cellular metabolic and protein quality data in real time to support process intensification and modelling.

Conference Talk: 2021 Industrializing Cell-Based Meats & Seafood Summit

The Importance of Cell Media Analysis in Upstream Bioprocess Development

Watch this discussion with experts from industry and academia as they discuss bioprocessing trends relating to cell culture media analysis.

Mass Spectrometry-Based Process Analytical Technologies for Cell Therapies

Unlocking rapid cell culture media analysis for cell and gene therapy process development

Be a REBEL with Your Cell Media Analytics

Redefine Bioprocess Analytics

Rapid at-line amino acid and nutrient profiling from bioprocess media with the REBEL

How can we accelerate PD analytics?

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