Resource Hub
Application Notes & Snapshots
Application Snapshot Booklet Fermentation
Application Snapshot Booklet Cell and Gene Therapy
Application Snapshot Booklet Cell Culture
Application Snapshot Booklet Biologics
Application Snapshot Booklet
Download our full collection of application snapshots, with focuses on biologics, CGT, and fermentation.
How comparable are different vendors’ LB media?
High variations of analyte levels in Terrific broth (TB) media
Investigating the high nutrient variations in Super Optimal Broth
Cultures’ growth rates and productivities may differ when different vendor’s media are used in otherwise identical experiments.
Probing formulation differences in Super Optimal Broth
Similarities between different vendors’ 2X YT liquid media
Comparing standard microbial media from a single supplier
At-line spent media analysis is now a reality
To elevate process development decisions to real-time, researchers require new analytical platforms that simplify analysis and accelerate delivery of actionable intelligence.
Differences in fresh commercially available hybridoma media
Select the best CHO media from the start
Performing your own quick assays of the media you receive ensures consistency in the cell media fed to your cells.
Screening of chemically-defined media for suspension cultures of HEK293 cells
Across four vendors, there was a high variation in the fresh media composition.
Analyze media without removing your mAb on the Rebel
Identify variations in dry and liquid CHO media
Running a quick fresh media assay prior to feeding the culture ensures consistency of the media components when scaling up or during the transition between liquid-to-dry media blends.
Serum supplemented media analysis is routine with the Rebel
Tease out variations in dry cell culture media
Running a simple media assay prior to feeding your cells can ensure that the composition of the media is what is expected.
Supplementing RPMI media with fetal bovine serum
Supplementing IMDM media with fetal bovine serum
Conventional and chemically-defined T cell medias
Amino acid contents of Sf9 and Sf21 insect cell media
Knowledge of the insect cell metabolism coupled with an understanding of the amino acid content of the cell media allows for a more informed process development analytics strategy.
Comparing mesenchymal stem cell media with supplements on the Rebel
To ensure consistency when culturing MSCs, researchers should understand what is being added to their media.
Don’t sweat your cell media additives
Handle non-ionic surfactants in cell media samples with ease
Basal cell media with 10% fetal bovine serum
Brochures & Flyers
REBEL Spent Media Analysis Kits
The REBEL Spent Media Analysis (SMA) kit is designed to give you all the consumable components needed to run your samples quickly and move your projects forward faster.
REBEL Brochure
The REBEL is a novel, dedicated cell culture media analyzer designed for rapid, easy, and efficient amino acid and nutrient analysis.
Podcasts
Posters
Optimization of Scalable rAAV Production for Gene Therapy
This poster showcases spent media analysis leveraging REBEL, which requires only 10 μL per sample and 10 minutes analysis time.
Empowering Process Optimization for a mAb-Expressing CHO Cell Line
Coupling miniature mass spectrometry (MS) with microchip capillary electrophoresis (CE) fulfills an unmet need: simple, rapid at-line nutrient quantitation.
Using CE-HPMS For Accelerated Process Development of a mAb-Expressing Cell Line
The coupling of miniature mass spectrometry (MS) to a microchip capillary electrophoresis (CE) platform provides a simple solution for at-line amino acid quantitation.
Dynamic Range Optimization for Amino Acid Quantitation with an Integrated CE-MS Analyzer
The coupling of miniature mass spectrometry (MS) to a microchip capillary electrophoresis (CE) platform provides a simple solution for amino acid quantitation.
Rapid at line quantitation of key nutrients in cell culture media
This poster details the benefits of coupling of miniature mass spectrometry to a microchip capillary electrophoresis platform for characterizing cell media metabolites in fresh and spent media.
Quantitation of metabolites in classical and customized cell culture media by an integrated CE-MS Analyzer
Quantitation of metabolites in cell culture media by an integrated CE-MS analyzer for upstream process development
Using genomic and spent media analysis to characterize stressed CHO cultures
A Rapid Microfluidic Method for Molecular Weight Determination and Spent Media Analysis of an IgG1 Intact Protein in Growth Media
Characterizing the Analytical Performance and Reliability of Metabolite Quantitation by an Integrated CE-MS Analyzer
Rapid Analysis of Cell Therapy Media
Quantitation of CHO media nutrients and metabolites in under 180 seconds
Rapid analysis of amino acids, water soluble vitamins, biogenic amines and dipeptides in cell growth media
Rapid at line nutrient profiling from an ammonia stressed CHO cell line
Published Articles
In-Process Analytics are Key to Successful Cell Therapy Process Optimization and Manufacturing
Considerable clinical activity and the approval of several autologous, patient-specific therapies has increased industry demand and has also highlighted bottlenecks in manufacturing workflows.
Spent Media Analysis of CHO Grown in an Ammonia-Stressed Parallel Microbioreactor Platform
Safety Data Sheet (SDS)
SDS – REBEL BGE
Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product.
SDS – REBEL Diluent, SMA
Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product.
SDS – REBEL Standard
Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product.
Specification Sheets
Videos
Conference Talk: Power Up Your Process Control (BPI 2022)
Graziella Piras spoke at BPI 2022 about how amino acids can present a goldilocks problem for bioprocessing: too little and they starve; too much and they favor growth over protein production.
REBEL Product Video
Poster Chat: Spent Media Analysis of CHO
Rapid at-line nutrient profiling from an ammonia stressed CHO cell line
Booth Chat
Demo Video
Rapid analysis of amino acids, water soluble vitamins, biogenic amines and dipeptides in cell growth media
Webinars
Harness the Power of At-Line Spend Media Analysis to Optimize a CHO mAb Fed-Batch Process
This webcast shares a data-driven and accelerated cell culture media screening as well as the selection in the Ambr15 microbioreactor system.
Multi-Component At-Line Cell Culture Media Analysis
In this talk, Graziella Piras discusses a data-driven and accelerated media screening, selection, and optimization experiment leveraging REBEL.
Power up your process control with rapid at line MS-based key nutrient monitoring
Graziella Piras, bioprocess segment director for 908 Devices, spoke at BPI Europe 2022 on the benefits of at-line nutrient monitoring.
The Critical Role of Cell Culture Media Analysis in CGT Development
Does cell culture analysis matter for CGT development? This short webinar explores what is already known, reviews at-line methods for cell culture media analysis, and shares recent data from two case studies.
Biopharma 4.0: Finding Opportunities to Leverage New At-Line Data
Listen how REBEL users describe how they use better data acquisition, management platforms, and process modeling to implement process intensification.
Process Modeling with Researchers from Johns Hopkins University
Listen to a dynamic discussion on the highlights of a new peer-reviewed article exploring model-based approached to cell culture optimization
![[Webinar] Bioprocess 101: Cell Culture Media Analysis](https://908devices.com/wp-content/uploads/2022/01/9080151_Bioprocess101-Cell-Culture-Analysis_R1-300x300.jpg)
Ask the Expert: Bioprocess 101 – Cell Culture Media Analysis
Process Intensification: Do You Have All the Data You Need When You Need It?
The need for process intensification and implementation of Bioprocess 4.0 requires extensive real time data acquisition and process modelling. At line MS-based technologies presented here provide key cellular metabolic and protein quality data in real time to support process intensification and modelling.
Conference Talk: 2021 Industrializing Cell-Based Meats & Seafood Summit
The Importance of Cell Media Analysis in Upstream Bioprocess Development
Watch this discussion with experts from industry and academia as they discuss bioprocessing trends relating to cell culture media analysis.
Mass Spectrometry-Based Process Analytical Technologies for Cell Therapies
Unlocking rapid cell culture media analysis for cell and gene therapy process development
Be a REBEL with Your Cell Media Analytics
Redefine Bioprocess Analytics
Rapid at-line amino acid and nutrient profiling from bioprocess media with the REBEL
How can we accelerate PD analytics?
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