REBEL Brochure
The REBEL is a novel, dedicated cell culture media analyzer designed for rapid, easy, and efficient amino acid and nutrient analysis.
BOOST YOUR BIOPROCESS
CELL CULTURE MEDIA ANALYSIS IN MINUTES, NOT WEEKS
CELL CULTURE MEDIA ANALYSIS MATTERS
In upstream bioprocessing, cell culture media development is a key area of focus, and monitoring of the media all the way through manufacturing is critical. Understanding the relationship between cell culture media components and the cells they support can ultimately impact the process Key Performance Indicators (KPIs) and Critical Quality Attributes (CQAs). Analysis of key media components such as amino acids, vitamins, and the metabolites produced as a result of cell growth is beneficial at all stages of process development, including
cell line development, media QC, and media development. This information provides insight not just on media composition and performance, but also on clone selection, feed strategy, optimal harvest time, product quality, and more. The need for more process intensification and process modeling requires frequent real time data that can easily be used for deeper monitoring and control.
Ultimately, having greater insight offers more opportunities to positively impact CQAs and KPIs to create products faster, more cost effectively, and with greater therapeutic success.
GETTING RESULTS ISN’T ALWAYS EASY
Process development groups don’t always have the analytical tools or resources to gain in-depth process understanding. Sending samples to on-site or off-site core labs can take days to weeks and can be costly.
MEET THE
REBEL
The REBEL is a novel, dedicated cell culture media analyzer designed for rapid, easy, and efficient amino acid and nutrient analysis. Combining award winning design with 908 Devices proven capillary electrophoresis and high-pressure mass spectrometry technologies, the REBEL provides process development scientists access to critical data at-line, any time.
ANALYSIS IN 4 EASY STEPS
PULL A SAMPLE
Pull a sample from your bioreactor, shake flask, or well plate. The REBEL only needs 10µL.
SPIN & DILUTE
Spin out your cells and dilute. That’s as complicated as it gets.
LOAD YOUR SAMPLES
Load your samples, reagents, and calibrants.
GENERATE RESULTS
Hit start, get results. Results can quickly be reviewed in a simple report.
REBEL
BENEFITS
EASY TO OPERATE
Designed for simplicity.
MINIMAL SAMPLE PREPARATION
Pull a sample from your bioreactor, shake flask or well plate, then spin out your cells and dilute.
NO MORE WAITING FOR RESULTS
Fully processed at line report for 30+ components in under 10 minutes.
DATA INTEGRATION
For easy data alignment and interpretation with third-party platforms.
A COMPREHENSIVE PANEL TO MEET YOUR NEEDS
THE REBEL SCREENS FOR OVER 30 ANALYTES IN EVERY RUN, ALL IN UNDER 10 MINUTES
AMINO ACIDS
- Alanine
- Alanyl-Glutamine
- Arginine
- Asparagine
- Aspartic Acid
- Cystine
- Glutamic Acid
- Glutamine
- Glycine
- Histidine
- Isoleucine
- Leucine
- Lysine
- Methionine
- Isoleucine
- Leucine
- Lysine
- Methionine
- Phenylalanine
- Serine
- Threonine
- Tryptophan
- Tyrosine
- Valine
VITAMINS
- Choline
- Nicotinamide
- Pyridoxal
- Pyridoxine
- Thiamine
AMINES
- β-Alanine
- Citrulline
- GABA
- Hydroxyproline
- Methyl-Histidine
- Sarcosine
KNOW WHAT’S IN YOUR MEDIA
Understanding which key nutrients are present in your cell culture media is critical to media screening and optimization efforts. In this experiment, nine commercial CHO media were measured using the REBEL in a media screening evaluation (Figure 1). The amino acid profile reveals high diversity in nutrient composition across the media panel. With the REBEL you can measure key nutrients such as amino acids in both fresh and spent media at-line in real time to gain deeper insight into your process and obtain high titer faster.
CHO MEDIA SCREENING
Analyzed using a simple 100x dilution with 20 replicates.
Figure 1. Diversity of nutrient composition in a panel of chemically defined CHO media.
REBEL measures over 30 analytes including all amino acids, certain vitamins, and bio amines. Plotted here are a subset of amino acids including all essential ones and others in 9 different CHO media. This comparison shows that the concentration of several amino acids such as serine and arginine, two potentially important amino acids for cell growth and protein production, varies widely between the media tested here while other amino acids are more consistent between media.
COMPACT WITHOUT COMPROMISE
The REBEL delivers high method accuracy and reproducibility, ensuring confidence in results. In a spiking experiment, 17 analytes with known concentrations were added into a chemically defined medium and measured using the REBEL. Values obtained with REBEL showed high accuracy (values were within +/- 20% of expected values) and high analytical precision (%RSDs were between 7 to 16%) as demonstrated in Figure 2.
AMINCO ACID RECOVERY IN CHO MEDIUM
Figure 2. Recovery of analyte concentration using the REBEL analyzer. 17 analytes (Sigma AAS18 amino acid standard) were spiked at 1mM and cystine at 0.5mM, into a commercial chemically defined medium in triplicate and measured four times on the REBEL (total of 12 replicates). All analytes were between 93 –118% of expected values.
HOW WILL YOU USE
REBEL?
MONITOR AMINO ACIDS AND VITAMINS THROUGHOUT YOUR BIOREACTOR TIME COURSE. REFINE FEEDING STRATEGIES ON THE FLY. CORRELATE MEDIA SELECTION TO PRODUCT QUALITY AND YIELD.
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