Resource Hub
Application Notes & Snapshots
Application Report: Glucose Control
The online analyser TRACE C2 Control allows a rapid and precise determination of glucose and lactate concentrations inside the bioreactor.
Application Report: Ethanol Dialysis
The online analyzer TRACE C2 Control allows a rapid and precise determination of the Ethanol concentration in the fermenter.
Application Report: Methanol Dialysis
The online analyzer TRACE C2 Control allows a rapid and precise determination of the methanol concentration in the fermenter and provides the tools for a quick set-up of a feed control strategy
Application Report: Glucose & Lactate (in Small Bioreactors)
The online analyser TRACE C2 Control allows a rapid and precise determination of glucose and lactate concentrations inside small bioreactors.
Application Report: Glucose & Lactate
The online analyzer TRACE C2 Control allows a rapid and precise determination of glucose and lactate concentrations inside the bioreactor.
Glucose and Lactate Raman-Based Monitoring with MAVERICK: Performance in Spent Media
MAVERICK provides a plug-and-play, in-line, Raman based PAT solution for monitoring and control of glucose, lactate, and biomass in up to 6 bioreactors. This Application Snapshot shows the results from experiments to demonstrate the selectivity of glucose and lactate measurements from MAVERICK in complex spent media samples from CHO and HEK293 cultures.
Brochures & Flyers
REBEL Certified User Training
Get the best user experience with the REBEL at-line spent media analyzer. Our training course is for new and experienced users who want to refresh their REBEL device
operation skills.
MAVERICK Brochure
Real-time bioprocess monitoring and control, right out of the box. In-line measurements of multiple critical parameters across media, across processes, across mammalian cell lines, across scales.
Posters
Automated Glucose Feeding Based On Online Glucose And Lactate Measurements
This poster note describes an automated control strategy for cell growth and biologics production. It leverages MAVEN, a reliable, easy-to-operate, online (sample-free) device for monitoring of glucose and lactate.
Process Optimization by the Use of Substrate Feeding Strategies
Online glucose monitoring is possible by using Trace C2 Control system. Feed control without online glucose monitoring causes acetate formation due to overflow metabolism.
Optimized Fed-Batch Fermentation by Use of Online Glucose Measurement
This poster presentation features an integrated multivariate glucose measuring tool combined with software solutions which allow different types of feeding-strategies.
High Frequency Glucose and Lactate Measurements in Multi-Bioreactor Vessels
TRACE C2 is an online analyzer for monitoring and controlling glucose and lactate concentrations in bioreactors. With the extension M4 it is possible to monitor Glucose and Lactate in up to four simultaneous bioprocesses.
Online-Controlling of Glucose in Cultivations and Fermentations
TRACE C2 Control is a new online analyzer for monitoring and controlling of glucose concentrations in bioprocesses. In disturbance reaction experiments and in an E.coli fermentation it can be successfully shown that a reliable control is possible.
Implementation of a New Method for Online Glucose Control in a 10L CHO Bioprocess
This poster shows how glucose concentration can be directly measured based on an enzymatic reaction. This method is then used to control the glucose level in a fed-batch fermentation at a
constant level.
Single-Use Bioreactor for High Density Bacterial Fermentation
Glucose measurement is performed on-line using the TRACE C2 analyser. This allows to follow-up glucose uptake rate and monitor fed batch culture with retro-control on the glucose pump such as maintain glucose level at predefined set point.
Empowering Process Optimization for a mAb-Expressing CHO Cell Line
Coupling miniature mass spectrometry (MS) with microchip capillary electrophoresis (CE) fulfills an unmet need: simple, rapid at-line nutrient quantitation.
Using CE-HPMS For Accelerated Process Development of a mAb-Expressing Cell Line
The coupling of miniature mass spectrometry (MS) to a microchip capillary electrophoresis (CE) platform provides a simple solution for at-line amino acid quantitation.
Dynamic Range Optimization for Amino Acid Quantitation with an Integrated CE-MS Analyzer
The coupling of miniature mass spectrometry (MS) to a microchip capillary electrophoresis (CE) platform provides a simple solution for amino acid quantitation.
Rapid at line quantitation of key nutrients in cell culture media
This poster details the benefits of coupling of miniature mass spectrometry to a microchip capillary electrophoresis platform for characterizing cell media metabolites in fresh and spent media.
Published Articles
Specification Sheets
Tech Notes
Instant Implementation of Raman-based PAT with MAVERICK for Monitoring Glucose and Lactate
MAVERICK is easy to set up and enables instant implementation of PAT generating accurate, precise, linear and selective in-line measurements across a wide range of media used typically in mammalian (including CHO and HEK293 media) cell culture for the production of biotherapeutics.
Determining Total Cell Count with MAVERICK Biomass Measurements
MAVERICK provides an in-line, Raman-based PAT solution for monitoring and control of glucose, lactate, and biomass in up to six bioreactors right out of the box.
On-line Measurement of Glucose and Lactate with Automated Feedback Control
MAVEN answers the urgent need for on-line measurements and automated control of glucose levels, which reduces manual labor, lowers risk of contamination from sampling, decreases risk of human error with feeding, and reduces the stress that cells may experience from variable glucose levels.
Technical Papers
Tech Note: Glucose & Lactate Dialysis (Low)
These data were compiled in order to give an overview of typical system and sensor performance in the low concentration range using the dialysis-low sampling method. Operational temperature in the bioreactor was set to 37°C.
Tech Note: Glucose & Lactate Dialysis
These data were compiled in order to give an overview of the system and sensor performance in the normal concentration range using the dialysis sampling method.
Tech Note: Pressure Stability of the Dialysis Probe
The TRACE dialysis probe can be safely used in large reactors. Its dialysis membrane does not suffer from higher pressure due to hydrostatic and/ or pressurized conditions.
Tech Note: Interference of Other Sugars on Glucose and Lactate Measurements
Most sugars have no influence on glucose measurement. The only exception is mannose, which shows a low positive interference on the glucose measurement.
Videos
MAVERICK: Raman-based Monitoring of Multiple Critical Parameters, Ready to Go in Minutes
MAVERICK is powered by Raman spectroscopy and purpose-built de novo model for simultaneous real-time measurements of multiple key process parameters in mammalian cell cultures without the costs, complexities, and risks of conventional methods.
MAVERICK: Monitoring of Multiple Bioprocess Parameters Right out of the Box
There’s no need to deal with complex empirical calibration models, reagents, or unreliable auto-sampling systems to implement in-line bioreactor monitoring.
REBEL Customer Experience: Clemson University
Listen to Stephanie Klaubert, a graduate research assistant from Clemson University, as she shares how REBEL helps her with process development and cell culture optimization.
Conference Talk: Power Up Your Process Control (BPI 2022)
Graziella Piras spoke at BPI 2022 about how amino acids can present a goldilocks problem for bioprocessing: too little and they starve; too much and they favor growth over protein production.
MAVEN Product Video
If glucose and lactate measurements run your lab’s schedule, it’s time to meet MAVEN.
Webinars
In-line Monitoring of Bioprocess Analysis Parameters for the 21st Century
Now Starts Now: The Future of Glucose & Lactate Measurement
Learn how MAVEN addresses the challenges of on-line nutrient and metabolite monitoring and control.
Reduce Process Development Timelines and Improve Productivity
This webcast shares a data-driven and accelerated cell culture media screening as well as the selection in the Ambr15 microbioreactor system.
Lower Costs, Accelerate Productivity and Time-to-Market with Fast Analytical Tools
Learn how to take charge of your bioprocess development via at-line amino acid analysis empowered by a dedicated bioprocess software tool.
Know your Cells Nutrient Requirements and Accelerate Media Development Timelines
In this talk, Graziella Piras discusses a data-driven and accelerated media screening, selection, and optimization experiment leveraging REBEL.
Add the Missing Pieces to your Predictive Model with Rapid Amino Acid Profiling
Graziella Piras, bioprocess segment director for 908 Devices, spoke at BPI Europe 2022 on the benefits of at-line nutrient monitoring.
Add the Missing Pieces to your Predictive Model for Smarter DOEs
Listen how REBEL users describe how they use better data acquisition, management platforms, and process modeling to implement process intensification.
Process Modeling with Researchers from Johns Hopkins University
Listen to a dynamic discussion on the highlights of a new peer-reviewed article exploring model-based approached to cell culture optimization
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