ESACT | Edinburgh, UK | June 23-26

The European Society for Animal Cell Technology (ESACT) is a Society which brings together scientists, engineers and other specialists working with animal cells to promote communication of experiences between European and International investigators and progress the development of cell systems and productions derived from them. ESACT fosters collaboration and knowledge sharing in the field of animal cell technology. This field plays a crucial role in developing cell-based therapies, vaccines, and other products derived from animal cells.

Learn more about the ESACT Conference.

908 Devices is helping enhance process understanding, optimizing process performance, and ensuring batch-to-batch reproducibility in cell therapy manufacturing with easy-to-use PAT tools.

Join Us For Lunch at Our Seminar

Simple and readily deployable Process Analytical Technology (PAT) solutions for comprehensive monitoring and analysis of upstream monoclonal antibody production

Monday, June 24th
12:45 PM – 13:45 PM

Robust and informative PAT solutions supporting upstream production of biopharmaceutics are required to improve efficiency. Despite considerable interest in spectroscopic in-line techniques like Raman, the obstacles associated with constructing and validating models have impeded their widespread adoption. The deployment of specific methods that provide quantitative information on individual process components or product quality attributes is still challenging. Process analytics that provide direct quantitative readouts enable better process understanding, improvements, and modelling, as well as building and validating additional spectroscopy chemometric models. Here, we applied in-line Raman spectrometry using a novel approach for key process parameters, at-line amino acid cell culture analyzer for key media component analysis, and a CE-ESI interface coupled to a mass spectrometer for rapid product quality assessment. We present a solution for rapid generation of informative data, both in depth in quality and quantitation, and width of analytical coverage and frequency of measurements in the manufacturing process. We discuss effective implementation of easy-to-deploy PAT solutions. By integrating at-line media profiling data, in-line analysis of key process parameters, and fast protein quality attributes, this enables generation of near real-time insights into the interaction between the process and the product. This facilitates quicker and more robust process development.

Presenters

Jonathan Bones
PhD MBA
NIBRT

Jonathan Bones PhD MBA is a recognized leader in the field of biopharmaceutical characterization. Specializing in the development and application of liquid phase separations coupled to high-resolution mass spectrometry, Bones’ laboratory has become a go-to for those seeking analytical assistance with the various challenges encountered in the development and manufacture of biopharmaceuticals, including advanced therapeutic medicinal products. Jonathan is also the Director of CONCEPT, the SFI funded core facility for early-stage development of biotherapies at NIBRT.

Milla Neffling
PhD
908 Devices

Sr Manager, Bioprocess Applications, at 908 Devices, earned her doctorate in Finland at Åbo Akademi University in analytical biochemistry. She has a focus in analytical development in life sciences and biochemistry, and especially in mass spectrometry technology. In her current role, she is consulting and developing applications for Biopharmaceuticals and bioproduction with process development analytical solutions, such as MS-, biosensor-, and Raman-enabled technologies.

Stephen Driscoll
PhD
908 Devices

Dr. Stephen Driscoll is a senior scientist at 908 devices, specializing in the development and application of multivariate algorithms. During his doctoral research at Dalhousie University, Dr. Driscoll focused on the advancement of algorithms and evaluation techniques for multivariate chemical subspace estimation. He has made significant contributions to the mathematical understanding and modeling of long-range correlated error structures in analytical measurements. With a strong foundation in mathematical modeling and algorithm development, Dr. Driscoll now applies his expertise at 908 devices. He continues to push the boundaries of multivariate analysis by further refining existing algorithms and spearheading the development of novel approaches.

908 Devices Posters at the Conference

Poster Session 1
Monday, June 24
2:30-4:00PM

In-depth metabolic characterization at different phases of cell culture with a turnkey CE-ESI-HRMS workflow
Poster # 253

Raman-based Lactate Feeding Impact on Culture Characteristics and Product Quality in Mammalian Cell Cultures
Poster # 358

De Novo Approaches for Bioprocess Parameter Estimation using Raman Spectroscopy
Poster # 391

Poster Session 2
Monday, June 24th
7:30-9:30PM

Optimization of scalable rAAV-production for gene therapy – leveraging at-line amino acid measurements
Poster # 95

Detecting Microbial Cell Culture Contamination by Measuring Nicotinic Acid to Nicotinamide Ratio At-Line
Poster # 293

Data-driven feed strategy optimization and scale-up of mAb-expressing CHO cell line bioprocess
Poster # 314

Poster Session 3
Tuesday, June 25
2:30-4:00PM

Spent media analysis aids in determining metabolic mechanisms of superior CAR T-cell products
Poster # 96

New Era of Real-time Bioprocess Monitoring and Control –Raman-based Direct Measurements with De Novo Model
Poster # 390