Charge variant separations like icIEF and CEX are great, but without mass spec analysis how do you really know what you’re seeing? ZipChip® performs charge variant analysis of biotherapeutics and gives you more than just a separation. Here’s the big difference: It adds another layer of information with the power of mass spec characterization. More data with every run means that every variant is an identified variant.Suggested Resources
The ZipChip’s charge variant assay alone gives you more information than other techniques. The same goes for other characterization assays. Whether you’re running intact charge variant analysis, reduced analysis, or peptide mapping, ZipChip works at every level.Suggested Resources
ZipChip separates subtly different protein variants to characterize complex molecules like fusion proteins, bispecifics, glycoproteins, or even a mix of proteins in one sample. Chips and assay kits can handle complex sample matrices so detergents aren’t a problem either.Suggested Resources
ZipChip lets you take on anything from bioreactor media to metabolites in biofluids like plasma or urine. Sample prep is simple. There’s no labeling or derivatization, and no dry down when your sample matrix has organics. Add internal standards for full quantitation on a variety of analytes.Suggested Resources
What if your target analytes have the same mass? Your mass spec may not be able to tell the difference, but ZipChip can. It can separate isomers of small molecules and peptides based on subtle structural differences. That means target analytes are unequivocally identified every time.Suggested Resources
ZipChip is a front-end separation device that couples to your mass spectrometer. It joins capillary electrophoresis and electrospray ionization into a plug-and-play chip. There is minimal sample preparation and tiny injection volumes, so there is not much assistance needed by a scientist.
The beauty of the ZipChip is that you can perform large molecule (e.g., mAb’s) analysis in the morning and transition to small molecule (e.g., amino acids) analysis in the afternoon. It’s that fuss-free! There is no need to change mobile phases or columns to accommodate a new project.
Yes! ZipChip performs native charge variant separations by capillary-zone electrophoresis mass spectrometry (CZEMS). Not only can you separate the charge variants (basic lysine 2K and 1K, 0K monomer, and acidic/deamidated species), the selectivity of the mass spectrometer allows you to determine the accurate mass of each species by deconvoluting the charge envelop of each peak.
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