Our ZipChip CVA kit takes the guesswork out of method development. Characterizing your samples with ZipChip capillary electrophoresis coupled to your high-resolution MS, you're able to assess charge heterogeneity, identify basic and acidic variants, acquire accurate mass information, and profile glycoforms, all in a single analysis.
Get more data and streamline your Charge Variant Analysis workflow with ZipChip CVA kit
Easy sample prep and rapid analysis
Little-to-no method development
High performance analysis with full charge variant separation and peak identification
Comparable profiles to commonly used CVA techniques with the added power of mass spec characterization
Dilute Sample and Prepare BGE
Load Sample into ZipChip
Run the CVA on ZipChip-MS
Get straight to it.
Our preconfigured ZipChip CVA kit takes the guesswork out of method development.
SEAMLESS WORKFLOW
ZipChip-MS peak profile is comparable to Capillary Isoelectric Focusing (CiEF), Cation Exchange Chromatography (CEX), and Capillary Zone Electrophoresis (CZE-UV) and delivers seamless workflow for identification of detected unknown variant peaks.
STREAMLINED DESIGN
Analyze multiple biotherapeutic protein CQAs in a single assay - gain the ability to clearly separate and identify acidic and basic species and assess the glycosylation profile.
OPTIMIZED FOR YOU
Characterize a variety of monoclonal antibodies (mAbs) and other biotherapeutic proteins with the ZipChip CVA kit without having to reoptimize your method.
ZipChip CVA Consumables
ZipChip CVA consumables provide everything you need for analysis with no method development and minimal sample prep.
Name
Contents
Sample per kit
Part No.
CVA Assay Kit
CVA Background Electrolyte (BGE), Diluent, and Additive
From complex biologics to small molecules, ZipChip has an assay kit for you.
Take the Guesswork Out of Method Development
3 Easy Steps to Selection
1. Application
2. Chip
3. Kit
Assay kits come with background electrolyte and sample diluent that’s optimized for each application. Just put the bottle of background electrolyte in the autosampler, prep your samples with the sample diluent and you’re off.
1. The Application
From large to small molecule, ZipChip’s got you covered.
Protein Characterization: ZipChip is a one-stop shop for CQA’s—from charge variant analysis to peptide mapping. You’ll get high-resolution separation coupled to MS with simple, streamlined workflows.
Metabolomics: Quickly analyze small polar analytes from a variety of matrices, or monitor dynamic levels of metabolites in real time. Sample prep is quick and easy and results are ready in minutes.
Oligonucleotides: The characterization of nucleic acid-based drugs like oligonucleotides isn’t easy. ZipChip can offer some advantage over LC separations, particularly variations due to charge or shape.
Small Molecules: Analysis is easy and fast with ZipChip. Simply dilute and shoot; there’s no labeling or derivatization. Small polar analyte assays take as little as 2 minutes. Add internal standards for full quantitation on a variety of analytes.
2. The Chips
Choose from a range of chip options to optimize your workflow. Each chip can be used up to 125 injections.
HS: High Speed chip enables fastest separations. Matching BGE kits: Intact Antibody, Peptides, or Metabolites.
HR: High Resolution chip for applications demanding maximum separation capacities. Matching BGE kits: Intact Antibody, Peptides, or Metabolites.
HSN: High Speed chip for fast native protein analysis. Matching BGE kits: Native Antibodies or Charge Variant Analysis.
HRN: High Resolution chip with ultimate separation for native protein analysis. Matching BGE kits: Native Antibodies, or Charge Variant Analysis.
HSB: High Speed Bare Glass chip for high throughput separations. For example: fast oligonucleotides analysis. Matching BGE kit example: Oligos BGE.
HRB: High Resolution Bare Glass chip for high resolution separation of negatively charged analytes such as oligonucleotides. Matching BGE kit example: Oligos BGE.
3. BGE Kits
Background electrolyte (BGE) and sample diluent reduce preparation time for your samples. With ZipChip you just dilute and shoot!
Charge Variant Analysis: Formulated to provide excellent charge variant separations and MS sensitivity for antibodies and other proteins on all mass spectrometers.
Native Antibodies: Excellent charge variant separation of antibodies and other constructs to generate native spectra.
Peptides: Fast, efficient separations of protein digests and peptides utilizing low pH.
Metabolites: Streamlined sample prep and high-speed analysis for small molecules in denaturing conditions at low pH.
Oligonucleotides: Rapid analysis of oligonucleotides without the use of ion-pairing agents.
Ordering Information
ZipChip Assay Kits*
Part Number
Samples Per Kit
Charge Variant Analysis Kit
850-00052
625
Native Antibody Assay
850-00048
625
Metabolites Assat
850-00033
625
Peptide Assay
850-00034
625
Oligos Assay
850-00066
625
Oligos Starter
850-00067
250 Tests
*All kits contain BGE and diluent, and chips need to be ordered separately. The exception is the oligos starter kit which contains BGE, Diluent, chips and a standard.
Chips
Part Number
Chips Per Box
Injections Per Chip
HS
810-00195
5
125
HR
810-00194
5
125
HSN
810-00230
5
125
HRN
810-00227
5
125
HSB
810-00237
5
125
HRB
810-00239
5
125
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HASSLE FREE
Oligo characterization with ZipChip
The newly released ZipChip® oligos kit provides a simple, sensitive, dilute-and-shoot method for rapid analysis of oligonucleotides, applicable to various sizes and modifications.
Benefits:
High-resolution accurate mass identification and quantitation with CE-MS
No ion-pairing agents; no instrument contamination
Orthogonal to LC-based separation
Analysis in minutes with simple sample prep
Part Name
Contents
Samples per kit
Part No.
ZipChip Oligos Starter Kit
Oligos Background electrolyte (BGE) x 2 Diluent x 2 HRB Chip x 2 Oligo standard
250
850-00067
ZipChip Oligos Kit
Oligos BGE x 5 Diluent x 5
625
850-00066
HSB Chip
High Speed Bare Glass Chip (HSB) x 5
625
810-00237
HRB Chip
High Resolution Bare Glass Chip (HRB) x 5
625
810-00239
ZipChip Oligos Standard
20 mer DNA Primer
-
445-02281
Rapid Characterization of Oligos
Separation of Modified ssRNAs
Electropherogram showing separation of biotinylated, glycosylated and phosphorylated 20 mers from the corresponding unmodified 20 mer RNA standard
Rapid characterization of ssRNA with no method development
Base Peak Electropherogram for 20mer unmodified ssRNA standards (left) along with the raw mass spectrum (right).
ZipChip leverages microfluidic-based Capillary Electrophoresis (CE) and electrospray ionization technology to simplify sample preparation, perform high-resolution separations with reduced analysis times, and directly introduce samples into a Thermo Fisher Scientific, Bruker, or Sciex mass spectrometer. Achieve faster results with the ZipChip CE-MS device.
CE-MS DEVICE
ZipChip
ACCELERATE YOUR MS CHARACTERIZATION
Rapid protein characterization and metabolomic analysis with microfluidic technology for highly efficient separations of complex samples.
BIOLOGICAL CHARACTERIZATION
IN MINUTES
GIVE YOUR MASS SPEC SOME ZIP
The ZipChip® platform prepares and separates a wide range of biological samples, then electrosprays them into your mass spec for analysis. Just clip it on. The process takes as little as a few minutes, and it results in better separation quality than most LCs in a fraction of the time. Simple workflows and multiple kit options cover a host of biotherapeutic, metabolomic, and proteomic applications.
EASY ON SAMPLES
Now the kicker. ZipChip plays well with your mass spec and your proteins. Gentle sample prep reagents preserve structural integrity without denaturing or unfolding proteins for fully native mass spectra. No need to remove detergents or desalt. Negative and neutrals are trashed. Only positive analytes head out for cleaner mass spectra and more identified peaks.
EFFICIENCY WITH ADVANCED
MICROCHIP TECHNOLOGY
WITH ZIPCHIP YOU GET THE DATA FASTER, AND BECAUSE OF THAT YOU CAN MAKE QUICK DECISIONS
Associate scientist at Amgen
Easy as 1-2-3
Pick an application, a chip, and a kit. After simply loading the premixed background electrolyte and sample into the autosampler, pop in a ZipChip, and hit run. Use vials or 96-well plates.
It’s hands-off from there. Smart chips tell the system which method to use based on your preferences. Samples are automatically loaded, separated, and electrosprayed. Integrated software cues the mass spec to start analysis.
Efficient Separations
Microfluidic technology integrates capillary electrophoresis (CE) and electrospray ionization (ESI) on the ZipChip. Junctions and dead volumes don’t exist so analytical quality stays high, and no injection bias improves repeatability.
1. Pick your application, kit, and chip
2. Simple sample prep
3. Load reagent and chip, then run
IT'S ALL IN THE CHIP
FROM LARGE TO SMALL MOLECULES,
ZIPCHIP’S GOT YOU COVERED
Protein Characterization:
ZipChip is a one-stop shop for CQAs—with streamlined CE MS workflows for Charge Variant Analysis of basic and acidic species, Rapid Intact Mass Analysis, Subunit Analysis, and Peptide Mapping for deeper characterization.
Oligonucleotides:
The characterization of nucleic acid-based drugs like oligonucleotides isn’t easy. ZipChip can offer some advantage over LC separations, such as no ion-pairing agents to contaminate the instrument
Small Molecules
Analysis is easy and fast with ZipChip. Simply dilute and shoot; there’s no labeling or derivatization. Small polar analyte assays take as little as 2 minutes. Add internal standards for full quantitation on a variety of analytes.
Metabolomics:
Detect, identify and quantitate small polar analytes from a variety of matrices like cell lysates or growth media and monitor dynamic levels of metabolites in real time. Sample prep is quick and easy and results are ready in minutes.
Our ZipChip CVA kit takes the guesswork out of method development. Characterizing your samples with ZipChip capillary electrophoresis coupled to your high-resolution MS, you're able to assess charge heterogeneity, identify basic and acidic variants, acquire accurate mass information, and profile glycoforms, all in a single analysis.
Get more data and streamline your Charge Variant Analysis workflow with ZipChip CVA kit
Easy sample prep and rapid analysis
Little-to-no method development
High performance analysis with full charge variant separation and peak identification
Comparable profiles to commonly used CVA techniques with the added power of mass spec characterization
Dilute Sample and Prepare BGE
Load Sample into ZipChip
Run the CVA on ZipChip-MS
Get straight to it.
Our preconfigured ZipChip CVA kit takes the guesswork out of method development.
SEAMLESS WORKFLOW
ZipChip-MS peak profile is comparable to Capillary Isoelectric Focusing (CiEF), Cation Exchange Chromatography (CEX), and Capillary Zone Electrophoresis (CZE-UV) and delivers seamless workflow for identification of detected unknown variant peaks.
STREAMLINED DESIGN
Analyze multiple biotherapeutic protein CQAs in a single assay - gain the ability to clearly separate and identify acidic and basic species and assess the glycosylation profile.
OPTIMIZED FOR YOU
Characterize a variety of monoclonal antibodies (mAbs) and other biotherapeutic proteins with the ZipChip CVA kit without having to reoptimize your method.
ZipChip CVA Consumables
ZipChip CVA consumables provide everything you need for analysis with no method development and minimal sample prep.
Name
Contents
Sample per kit
Part No.
CVA Assay Kit
CVA Background Electrolyte (BGE), Diluent, and Additive
