Resource Hub
Application Notes & Snapshots
Microchip CE/MS Peptide Mapping Workflow with Trypsin Digestion for the Analysis of Critical Quality Attributes of Monoclonal Antibodies
Peptide mapping is considered the gold standard for the analysis of biopharmaceuticals, including monoclonal antibodies.
Poster Note: Bottom-up analysis of progressively larger RNAs by CZE-MS and CZE-MS/MS
This project explores the merits of CZE as a potential alternative to liquid chromatography, as the front-end separation of choice for analyzing progressively larger RNAs with a bottom-up approach.
Poster Note: Multi-Level Expeditious Characterization of AAV Capsid Proteins using ZipChip
This poster note presents a novel approach to complete characterization of capsid viral proteins using microchip CE-MS for the characterization of AAVs.
Poster Note: Rapid Characterization of Oligos Using Microchip CE-MS without Ion-Pairing Reagents
Learn how ZipChip’s ready-to-use oligos BGE solution is free from ion-pairing reagents and makes switching between applications simple and easy.
Quick screening of intact antibody and antibody drug conjugates with integrated microfluidic capillary electrophoresis and mass spectrometry
This application note shows a method for rapid screening of the heterogeneity of monoclonal antibodies and antibody-drug conjugates by an integrated microfluidic capillary electrophoresis (CE) and mass spectrometry (MS) workflow.
Rapid characterization of oligonucleotides using microfluidic CE-MS by ZipChip
This application note presents a CE-MS analysis of oligos by a ZipChip coupled to a QExactive HF mass spectrometer. This positive ESI mode method utilizes a MS-friendly background electrolyte (BGE), that eliminates the use of ion-paring agents such as hexafluoro isopropanol or alkyl amines.
Charge variant analysis with ZipChip and maXisII
A ZipChip capillary zone electrophoresis system coupled to Bruker maXis II UHR-QTOF offers an alternative selectivity to separate charge variants prior to MS analysis and affords a more complete intact mass characterization without adding complexity or high salt eluents.
A ZipChip Based CZE-MS Analysis for Quick Assessment of Biotherapeutics
From comprehensive characterization to monitoring product degradation characteristics
Charge Variant Analysis Coupled to a SCIEX TripleTOF® 6600+ System Mass Spectrometer
High-Throughput Intact Mass Analysis of mAb Based Therapeutics
Analysis of Reduced mAbs and ADCs
Rapid Characterization of Intact mAb-based Biotherapeutics
Quick screening of intact antibody and ADC
ZipChip for profiling and characterization of antibody charge heterogeneity
Sciex Flexible Solution for MAM with ZipChip
Sciex Presents Charge Heterogeneity of Monoclonal Antibodies with ZipChip
Snapshot: Charge Variant Analysis
Charge Variant Analysis of Native Antibodies for In-depth Characterization of mAb-based Biotherapeutics
Bioreactor Monitoring via ZipChip
Snapshot: High-Throughput Intact Mass
Snapshot: High Throughput Charge Variant Analysis
Snapshot: Cell Culture Analysis
Brochures & Flyers
ZipChip Consumables Guide
Learn how to take the guesswork out of method development with ZipChip. Breeze through your selection in three quick steps.
Hassle-Free Oligo Characterization with ZipChip
The newly released ZipChip® oligos kit provides a simple, sensitive, dilute-and-shoot method for rapid analysis of oligonucleotides, applicable to
various sizes and modifications.
ZipChip Platform Brochure
Download our platform brochure to learn how ZipChip® platform prepares and separates a wide range of biological samples, then electrosprays them into your mass spec for analysis.
A comprehensive CE-ESI MS solution for BioPharma applications
Darwin Flyer
Case Studies
REBEL + ZipChip help HJB Overcome Scientific Hurdles and Optimize Processes in Biotherapeutic Development
In their search to accelerate R&D and reduce costs, HJB discovered the power of REBEL and ZipChip.
Testimonial – Zachary Kelley – University of Kentucky
Architecture of autoinhibited and active BRAF–MEK1–14-3-3 complexes
Analysis to unravel biosimilarity of therapeutic proteins
Automated monitoring of amino acids in cell culture media
Highly sensitive charge variant analysis of complex biopharmaceuticals
In-depth analysis of top selling biotherapeutics
Posters
Multi-omic Analyses Enabled by Microchip Capillary Electrophoresis and Trapped Ion Mobility Mass Spectrometry
Microchip capillary electrophoresis coupled to a trapped ion mobility mass spectrometry promises high “omic” coverage in impressively short time and with minimal sample preparation.
Bottom-Up Analysis of Progressively Larger RNAs by CZE-MS and CZE-MS/MS
In this poster from ASMS 2022, researchers from University of Connecticut and Bruker showed that capillary zone electrophoresis performed on a chip proved to be an effective alternative to liquid chromatography as a front-end separation system to analyze both pure samples and complex digestion mixtures of nucleic acids.
Multi-level Expeditious Characterization of Adeno-associated Virus (AAV) Capsid Proteins Using ZipChip Microfluidic CE Coupled with HRMS
In this poster from ASMS 2022, scientists from Thermo Fisher Scientific and 908 Devices present a novel approach using microchip CE-MS for the characterization of AAVs.
A Sensitive and High-Throughput Assay for Analyzing Peptide Exchanged MHCl by High-Resolution Native CZE-MS
In this poster from ASMS 2022, researchers from Genentech describe a sensitive and high-throughput assay using capillary-zone electrophoresis coupled to a high-resolution mass spectrometer for characterizing peptide-exchanged MHCI under native conditions.
Combining μSPE ZipChip CE with PRM-LIVE on a timsTOF Pro for Warp-speed Selectivity Profiling of Deubiquitinase (DUB) Small Molecule Inhibitors
In this ASMS 2022 poster, researchers from Dana Farber Cancer Institute and Harvard Medical School developed a ZipChip-based method for facilitating high-throughput identification
of new small molecule inhibitors.
Microchip CE-MS Analysis of Sialylated N-glycan Linkage Isomers
In this ASMS 2022 poster, Fudan University researchers show a ZipChip-based strategy for qualitative and quantitative analysis of sialylated N-glycan linkage isomers was developed.
CE-MS of Oligonucleotides using a ZipChip
This ASMS 2022 poster from Boehringer Ingelheim explores the use of ZipChip coupled to a high resolution mass spectrometer as an orthogonal method to chromatography for oligonucleotide analysis.
Glycosylation Profiling of mAB Subunits With CZE-MS
This ASMS 2022 poster from Bruker shows ZipChip coupled with high resolution mass spectrometry providing charge-based selectivity which can assist with glycoforms separation.
Rapid Characterization of Oligos and Related Impurities Using Microchip CE-MS Without Ion-pairing Reagents
This poster from ASMS 2022 describes a simple & fast method for oligonucleotide characterizations using a Microchip CE-MS platform.
Pushing the Boundaries of Speed and Sensitivity in Proteomics
To overcome sample loading limitations in CE-MS, the poster authors engineered a 1 mm SPE bed within the chip geometry, allowing 100-fold improvements in concentration sensitivity limit for CE-MS.
A Broad Application of Migration Time Indexing and Metabolite Libraries to Evaluate Quantitative Coverage in Microchip CE-MS Metabolomics
In the field of metabolomics, microchip capillary electrophoresis (mCE) has a number of potential advantages over traditional liquid chromatography (LC), including higher throughput due to decreased separation times, increased separation efficiency, and lower required sample volumes.
A Microchip CE-MS Based End-to-End Solution for the Analysis of Synthetic Oligonucleotides
This poster showcases a workflow using microchip CE-MS that is coupled with a vendor-neutral informatics platform
as a complete solution for the analysis of synthetic oligos.
Microchip CE-MS with Integrated Solid Phase Extraction
Microchip CE-MS of Oligonucleotides: What Can It Separate?
Fast Chip-CEMS for Characterization of Protein/PTM Changes
Characterization of ADCs using ZipChip
Combined Top-down and Bottom-up Proteomics
ZipChip for Glycomics and Glycoproteomics
Fast and Efficient Biopharmaceutical Characterization
Characterization of charge heterogeneity of mAb
Rapid, Multi-level Analysis of Complex Proteins
Oligonucleotide Analysis via Microfluidic CE-MS
Advancing mAb Characterization with Microchip CE-MS Couples to a PASEF Enabled QTOF
96 Bottles of Beer: Metabolic Profiling of Spent Growth Media
Native Analysis of Monoclonal Antibodies
Characterization of Protein/PTM Changes
Native CE-MS for Biopharmaceutical Characterization
A Rapid Microfluidic Method for Molecular Weight Determination and Spent Media Analysis of an IgG1 Intact Protein in Growth Media
Bioprocessing of Monoclonal Antibody Based Therapies
NIBRT Presents Native MS for Charge Variants using ZipChip
NIBRT Presents ZipChip for Native Charge Heterogeneity
Monitoring Amino Acid Composition of Cell Culture Media
Published Articles
The Critical Role of CE-MS Methods for Biotherapeutic Characterization
Capillary electrophoresis (CE) methods play an important role in biomolecular characterization while microfluidic CE-mass spectrometry (MS) accelerates analytical workflows and improves development/commercialization timelines.
Identification of AAV capsid proteins
Characterization of a SRPK1/2 Inhibitor Complex
Detection and Quantification of neurotoxin β-N-methylamino-l-alanine
Characterizing Covalent CDK Inhibitor Complexes
Rapid Fingerprinting of a Highly Glycosylated Fusion Protein
Analysis of monosaccharides, oligosaccharides and glycopeptides
Metabolite Profiling Reveals Predictive Biomarkers
Monitoring Glycosylation Profile and Protein Titer in Cell Culture Samples
Characterizing Antiviral Protein-Inhibitor Complexes
Characterization of NIST mAb Proteoforms and Glycoforms
In-depth analysis of monoclonal antibodies using microfluidic capillary electrophoresis and native mass spectrometry
Comparative Elucidation of Cetuximab Heterogeneity on the Intact Protein Level
Characterization of Protein Assemblies Related to Cancer Proliferation
Analysis of alkaloids in plant cell cultures
Safety Data Sheet (SDS)
SDS – Deoxyribonucleic Acid, Unmodified
SDS – ZipChip Standard: Amino Acids
SDS – ZipChip Interface Tray Grease
SDS – ZipChip Additive: Charge Variant Analysis
SDS – ZipChip BGE: Charge Variant Analysis
SDS – ZipChip Diluent: Charge Variant Analysis
SDS – ZipChip BGE: Intact Antibodies
SDS – ZipChip Diluent: Intact Antibodies
SDS – ZipChip Acid: Metabolites
SDS – ZipChip BGE: Metabolites
SDS – ZipChip Diluent: Metabolites
SDS – ZipChip BGE: Native Antibodies
SDS – ZipChip Diluent: Native Antibodies
SDS – ZipChip BGE: Oligos
SDS – ZipChip Diluent: Oligos
SDS – ZipChip BGE: Peptides
SDS – ZipChip Diluent: Peptides
Specification Sheets
ZipChip Hardware Specification Sheet
The compact interface seamlessly integrates all fluidics and electronics. Just clip it on your mass spec and off you go!
ZipChip Consumables Specification Sheet
Premixed background electrolyte and sample diluent reduce preparation time for your samples. With ZipChip you just dilute and shoot!
Tech Notes
How It Works
Performance Validation Study
Using the ZipChip Infusion Function
Using Field Strength Gradients
ZipChip and Detergents
ZipChip Protocols
We have compiled this list of concise protocols and technical notes to help you facilitate high-quality science with ZipChip. Simply log in to our customer support portal to view these files and more.
Evaluation of Sample Carryover
Testimonials
ZipChip Customer Experience: Symphogen
Listen to Dan Bach Kristensen, a principal scientist at Symphogen, as he shares how ZipChip helps him with charge variant separation.
Hear from Our Users: The Need for Speed in the Lab
Listen to ZipChip and REBEL users from The Dana Farber Cancer Institute, Sartorius, and Amgen discuss the impact of faster workflows in the lab.
Videos
ZipChip Customer Experience: Symphogen
Listen to Dan Bach Kristensen, a principal scientist at Symphogen, as he shares how ZipChip helps him with charge variant separation.
Interview: The Unprecedented Potential of CE-MS for Proteomics
Recorded at ASMS 2022, Michael MacCoss from the University of Washington and Will Thompson from 908 Devices discuss how ZipChip is addressing key challenges in proteomics, and its potential in diagnostics, investigating bacterial proteomes, and metabolite analysis.
Interview: The Key to Unsolved Insights into RNA Characterization?
Recorded at ASMS 2022, Dan Fabris from the University of Connecticut shares his experience with RNA characterization using ZipChip.
Interview: Oligonucleotide Analysis with ZipChip at Boehringer Ingelheim
Recorded at ASMS 2022, Vladimir Papov shares his experience with oligo analysis using ZipChip.
Whiteboard Explainer: Avoiding the Stickiness of Oligo Analysis
If you have worked with oligos before, you know this can be a challenging application. Fortunately, ZipChip offers a way to make workflows faster and easier.
Customer Experience: New England Biolabs
A Conversation with Sciex
A Conversation with Bruker
ZipChip. Put your mass into it
Application Experience: Native Analysis of mAbs and ADCs
Application Experience: Detergents
DYK: ZipChip Can See Charge Variants In Degraded Antibodies?
Do You Know ZipChip?
ZipChip Demo
ZipChip Booth Chat
ZipChip Poster Chat
Application Experience: Oligonucleotides
Webinars
Charge Variant Analysis for Intact Protein Characterization in a Biopharmaceutical Development Lab – Lessons Learned and the Road Ahead
Direct Microchip CE/MS Peptide Mapping Workflow for the Analysis of mAb CQAs
Microchip CE-MS technology, its capabilities, and typical application space.
A Versatile Platform for Small Molecules, Intact Proteins & Beyond
ZipChip can be used for applications ranging from proteins and peptides in biopharmaceuticals, to oligonucleotides and AAVs in cell and gene therapy applications.
EPO Separation & Analysis
Straightforward separation and analysis of a highly glycosylated protein
Oligo Analysis Simplified: Get Your Answers Faster with Microchip CE-MS
Simplifying Oligonucleotide Characterization with Microchip CE-MS
In this on-demand webinar, we present a microfluidic capillary zone electrophoresis-based separation coupled with HRAM mass-spectrometry for rapid characterization of oligos in positive ionisation mode.
Analysis of inhibitor-protein complexes and small molecules
Fast Therapeutic Protein Characterization
In-depth characterization of complex monoclonal antibodies and antibody-drug conjugates with native ZipChip CE-MS
Streamline Protein Characterization and Profiling with CE-ESI-MS
Why use capillary electrophoresis
Simultaneous monitoring of amino acids and protein titer
Redefine Bioprocess Analytics
Zip Through the Backlog
ZipChip for Rapid and Easy Nanospray Infusions
Summary: Streamlined protein characterization and profiling
Rapid, comprehensive biotherapeutics characterization
NIBRT presents ZipChip for biopharma characterization
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