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                    [post_title] => Microchip CE/MS Peptide Mapping Workflow with Trypsin Digestion for the Analysis of Critical Quality Attributes of Monoclonal Antibodies
                    [post_excerpt] => Peptide mapping is considered the gold standard for the analysis of biopharmaceuticals, including monoclonal antibodies.
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                    [post_title] => Rapid characterization of oligonucleotides using microfluidic CE-MS by ZipChip
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                    [post_content] => 

1. Application

2. Chip

3. Kit

[post_title] => ZipChip Consumables Guide [post_excerpt] => Learn how to take the guesswork out of method development with ZipChip. Breeze through your selection in three quick steps. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-consumables-guide [to_ping] => [pinged] => [post_modified] => 2024-08-30 12:00:02 [post_modified_gmt] => 2024-08-30 16:00:02 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=5077 [menu_order] => 279 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [1] => WP_Post Object ( [ID] => 4837 [post_author] => 36 [post_date] => 2021-10-29 12:17:00 [post_date_gmt] => 2021-10-29 16:17:00 [post_content] =>
  • High-resolution accurate mass identification and quantitation
    with CE-MS
  • No ion-pairing agents; no instrument contamination
  • Orthogonal to LC-based separation
  • Analysis in minutes with simple sample prep
[post_title] => Hassle-Free Oligo Characterization with ZipChip [post_excerpt] => The newly released ZipChip® oligos kit provides a simple, sensitive, dilute-and-shoot method for rapid analysis of oligonucleotides, applicable to various sizes and modifications. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-flyer-oligo-kit [to_ping] => [pinged] => [post_modified] => 2024-08-21 14:17:49 [post_modified_gmt] => 2024-08-21 18:17:49 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=4837 [menu_order] => 291 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [2] => WP_Post Object ( [ID] => 827 [post_author] => 36 [post_date] => 2018-06-04 04:28:30 [post_date_gmt] => 2018-06-04 08:28:30 [post_content] =>

ZipChip leverages microfluidic-based Capillary Electrophoresis (CE) and electrospray ionization technology to simplify sample preparation, perform high-resolution separations with reduced analysis times, and directly introduce samples into a Thermo Fisher Scientific, Bruker, or Sciex mass spectrometer. Achieve faster results with the ZipChip CE-MS device.

Protein Characterization:
Oligonucleotides:
Small Molecules
Metabolomics:
[post_title] => ZipChip: Accelerate Your MS Characterization [post_excerpt] => Rapid protein characterization and metabolomic analysis with microfluidic technology for highly efficient separations of complex samples. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-platform-brochure [to_ping] => [pinged] => [post_modified] => 2024-08-28 16:38:39 [post_modified_gmt] => 2024-08-28 20:38:39 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/resource/zipchip-platform-brochure/ [menu_order] => 329 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) ) [post_count] => 3 [current_post] => -1 [before_loop] => 1 [in_the_loop] => [post] => WP_Post Object ( [ID] => 5077 [post_author] => 36 [post_date] => 2021-12-17 09:39:33 [post_date_gmt] => 2021-12-17 14:39:33 [post_content] =>

1. Application

2. Chip

3. Kit

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                    [post_title] => 5’ mRNA Analysis By Microchip CE-MS Using an Internal Cleavage Motif for RNase H Digestion
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                    [post_title] => Rapid Identification of Conjugation Sites in Antibody Drug Conjugates Using Microchip Capillary Electrophoresis Coupled with Mass Spectrometry
                    [post_excerpt] => CE/MS method is optimized with NISTmAb digest as a reference control. Good MS/MS coverage of NISTmAb is obtained with narrow peak width and fast separation. This poster was presented as a collaboration with Bruker at ASMS 2023 as poster WP041.
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                    [post_title] => Fast Screening and Characterization of Therapeutic Peptide by Online Capillary Electrophoresis - Mass Spectrometry (CE-MS)
                    [post_excerpt] => Presenting a novel strategy for characterization of therapeutic peptides was developed based on microchip CE-MS and linkage-specific derivatization. This poster was presented in collaboration with Ningbo University at ASMS 2023 as poster TP544.
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                    [post_name] => fast-screening-and-characterization-of-therapeutic-peptide
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                    [post_modified] => 2024-03-21 11:02:12
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                    [post_content] => 
                    [post_title] => Determination of ATP, ADP, AMP, and Adenosine Levels by Microchip Capillary Electrophoresis Coupled with High Resolution Mass Spectrometry
                    [post_excerpt] => In this study, we present a novel ZipChip coupled with a QEHF for adenosine nucleotide analysis. This poster was presented at ASMS 2023 as poster TP489.
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                    [post_name] => determination-of-atp-adp-amp-and-adenosine-levels
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                    [post_title] => Peptide Mapping Workflow for Direct Microchip CE-MS Analysis of Biopharmaceuticals
                    [post_excerpt] => In this study we demonstrated the suitability of a modified digestion workflow for peptide mapping analysis via CE separation. This poster was presented at ASMS 2023 in collaboration with NIBRT. It was poster MP619.
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                    [post_name] => peptide-mapping-workflow-for-direct-microchip
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                    [post_content] => 
                    [post_title] => Bottom-Up Analysis of Progressively Larger RNAs by CZE-MS and CZE-MS/MS
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                    [post_name] => zipchip-poster-asms2022-uconn
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                    [post_title] => A Sensitive and High-Throughput Assay for Analyzing Peptide Exchanged MHCl by High-Resolution Native CZE-MS
                    [post_excerpt] => In this poster from ASMS 2022, researchers from Genentech describe a sensitive and high-throughput assay using capillary-zone electrophoresis coupled to a high-resolution mass spectrometer for characterizing peptide-exchanged MHCI under native conditions.
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                    [post_content] => 
                    [post_title] => CE-MS of Oligonucleotides using a ZipChip
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                    [post_name] => zipchip-poster-asms2022-boehringer
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                    [post_title] => Rapid Characterization of Oligos and Related Impurities Using Microchip CE-MS without Ion-pairing Reagents
                    [post_excerpt] => This poster from ASMS 2022 describes a simple & fast method for oligonucleotide characterizations using a Microchip CE-MS platform.
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                    [post_title] => A Microchip CE-MS Based End-to-End Solution for the Analysis of Synthetic Oligonucleotides
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                    [post_title] => Microchip CE-MS of Oligonucleotides: What Can It Separate?
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                    [post_title] => Fast Chip-CEMS for Characterization of Protein/PTM Changes
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                    [post_title] => Combined Top-down and Bottom-up Proteomics
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                    [post_title] => Rapid, Multi-level Analysis of Complex Proteins
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                    [post_title] => Advancing mAb Characterization with Microchip CE-MS Couples to a PASEF Enabled QTOF
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Posters

Microchip CE-MS Analysis of Nucleic Acids – From Characterization of Synthetic Oligonucleotides to Sequence Mapping of RNAs

This work will showcase how the ZipChip enables a simple and rapid workflow for the analysis of nucleic acids with noion-pairing reagents needed This poster was highlighted at the 2024 TIDES Conference.

Rapid In-Depth Characterization of Biologics by Microchip CE-MS: mAbs, AAVs and Nucleic Acids

Microchip capillary electrophoresis (CE) coupled with mass spectrometry (MS) has revolutionized the characterization of therapeutic modalities in biopharmaceuticals. Here, we present the applications of an integrated workflow that couples the microfluidic CE system (ZipChip) with MS in characterization of biotherapeutics. This was poster A03 at BPS Europe 2024.

5’ mRNA Analysis By Microchip CE-MS Using an Internal Cleavage Motif for RNase H Digestion

The subject of this poster was the investigation of an internal DNA motif to produce a shorter digestion product while simultaneously maintaining the length of the probe at 25nt for hybridization specificity. The shorter oligo results in easier detection using Microchip CE-MS approach. This poster was presented at ASMS 2023 as poster THP575.

Rapid Identification of Conjugation Sites in Antibody Drug Conjugates Using Microchip Capillary Electrophoresis Coupled with Mass Spectrometry

CE/MS method is optimized with NISTmAb digest as a reference control. Good MS/MS coverage of NISTmAb is obtained with narrow peak width and fast separation. This poster was presented as a collaboration with Bruker at ASMS 2023 as poster WP041.

Fast Screening and Characterization of Therapeutic Peptide by Online Capillary Electrophoresis – Mass Spectrometry (CE-MS)

Presenting a novel strategy for characterization of therapeutic peptides was developed based on microchip CE-MS and linkage-specific derivatization. This poster was presented in collaboration with Ningbo University at ASMS 2023 as poster TP544.

Determination of ATP, ADP, AMP, and Adenosine Levels by Microchip Capillary Electrophoresis Coupled with High Resolution Mass Spectrometry

In this study, we present a novel ZipChip coupled with a QEHF for adenosine nucleotide analysis. This poster was presented at ASMS 2023 as poster TP489.

Peptide Mapping Workflow for Direct Microchip CE-MS Analysis of Biopharmaceuticals

In this study we demonstrated the suitability of a modified digestion workflow for peptide mapping analysis via CE separation. This poster was presented at ASMS 2023 in collaboration with NIBRT. It was poster MP619.

Bottom-Up Analysis of Progressively Larger RNAs by CZE-MS and CZE-MS/MS

In this poster from ASMS 2022, researchers from University of Connecticut and Bruker showed that capillary zone electrophoresis performed on a chip proved to be an effective alternative to liquid chromatography as a front-end separation system to analyze both pure samples and complex digestion mixtures of nucleic acids.

A Sensitive and High-Throughput Assay for Analyzing Peptide Exchanged MHCl by High-Resolution Native CZE-MS

In this poster from ASMS 2022, researchers from Genentech describe a sensitive and high-throughput assay using capillary-zone electrophoresis coupled to a high-resolution mass spectrometer for characterizing peptide-exchanged MHCI under native conditions.

CE-MS of Oligonucleotides using a ZipChip

This ASMS 2022 poster from Boehringer Ingelheim explores the use of ZipChip coupled to a high resolution mass spectrometer as an orthogonal method to chromatography for oligonucleotide analysis.

Rapid Characterization of Oligos and Related Impurities Using Microchip CE-MS without Ion-pairing Reagents

This poster from ASMS 2022 describes a simple & fast method for oligonucleotide characterizations using a Microchip CE-MS platform.

A Microchip CE-MS Based End-to-End Solution for the Analysis of Synthetic Oligonucleotides

This poster showcases a workflow using microchip CE-MS that is coupled with a vendor-neutral informatics platform
as a complete solution for the analysis of synthetic oligos.

Microchip CE-MS of Oligonucleotides: What Can It Separate?

Fast Chip-CEMS for Characterization of Protein/PTM Changes

Combined Top-down and Bottom-up Proteomics

Rapid, Multi-level Analysis of Complex Proteins

Oligonucleotide Analysis via Microfluidic CE-MS

Advancing mAb Characterization with Microchip CE-MS Couples to a PASEF Enabled QTOF

Characterization of Protein/PTM Changes

NIBRT Presents ZipChip for Native Charge Heterogeneity

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Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

Deoxyribonucleic Acid, Unmodified

SDS Deoxyribonucleic Acid, Unmodified

Interface Tray Grease

SDS Interface Tray Grease

Standard: Amino Acids

SDS Standard: Amino Acids (EU)
SDS Standard: Amino Acids (UK)
SDS Standard: Amino Acids (USA)

Additive: Charge Variant Analysis

SDS Additive: Charge Variant Analysis (EU)
SDS Additive: Charge Variant Analysis (UK)
SDS Additive: Charge Variant Analysis (USA)

BGE: Charge Variant Analysis

BGE: Charge Variant Analysis (EU)
BGE: Charge Variant Analysis (UK)
BGE: Charge Variant Analysis (USA)

Diluent: Charge Variant Analysis

Diluent: Charge Variant Analysis (EU)
Diluent: Charge Variant Analysis (UK)
Diluent: Charge Variant Analysis (USA)

BGE: Intact Antibodies

BGE: Intact Antibodies (EU)
BGE: Intact Antibodies (UK)
BGE: Intact Antibodies (USA)

Diluent: Intact Antibodies

Diluent: Intact Antibodies (EU)
Diluent: Intact Antibodies (UK)
Diluent: Intact Antibodies (USA)

Acid: Metabolites

Acid: Metabolites (EU)
Acid: Metabolites (UK)
Acid: Metabolites (USA)

BGE: Metabolites

BGE: Metabolites (EU)
BGE: Metabolites (UK)
BGE: Metabolites (USA)

Diluent: Metabolites

Diluent: Metabolites (EU)
Diluent: Metabolites (UK)
Diluent: Metabolites (USA)

BGE: Native Antibodies

BGE: Native Antibodies (EU)
BGE: Native Antibodies (UK)
BGE: Native Antibodies (USA)

Diluent: Native Antibodies

Diluent: Native Antibodies (EU)
Diluent: Native Antibodies (UK)
Diluent: Native Antibodies (USA)

BGE: Oligos

BGE: Oligos (EU)
BGE: Oligos (UK)
BGE: Oligos (USA)

Diluent: Oligos

Diluent: Oligos (EU)
Diluent: Oligos (UK)
Diluent: Oligos (USA)

BGE: Peptides

BGE: Peptides (EU)
BGE: Peptides (UK)
BGE: Peptides (USA)

Diluent: Peptides

Diluent: Peptides (EU)
Diluent: Peptides (UK)
Diluent: Peptides (USA)

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Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

Deoxyribonucleic Acid, Unmodified

SDS Deoxyribonucleic Acid, Unmodified

Interface Tray Grease

SDS Interface Tray Grease

Standard: Amino Acids

SDS Standard: Amino Acids (EU)
SDS Standard: Amino Acids (UK)
SDS Standard: Amino Acids (USA)

Additive: Charge Variant Analysis

SDS Additive: Charge Variant Analysis (EU)
SDS Additive: Charge Variant Analysis (UK)
SDS Additive: Charge Variant Analysis (USA)

BGE: Charge Variant Analysis

BGE: Charge Variant Analysis (EU)
BGE: Charge Variant Analysis (UK)
BGE: Charge Variant Analysis (USA)

Diluent: Charge Variant Analysis

Diluent: Charge Variant Analysis (EU)
Diluent: Charge Variant Analysis (UK)
Diluent: Charge Variant Analysis (USA)

BGE: Intact Antibodies

BGE: Intact Antibodies (EU)
BGE: Intact Antibodies (UK)
BGE: Intact Antibodies (USA)

Diluent: Intact Antibodies

Diluent: Intact Antibodies (EU)
Diluent: Intact Antibodies (UK)
Diluent: Intact Antibodies (USA)

Acid: Metabolites

Acid: Metabolites (EU)
Acid: Metabolites (UK)
Acid: Metabolites (USA)

BGE: Metabolites

BGE: Metabolites (EU)
BGE: Metabolites (UK)
BGE: Metabolites (USA)

Diluent: Metabolites

Diluent: Metabolites (EU)
Diluent: Metabolites (UK)
Diluent: Metabolites (USA)

BGE: Native Antibodies

BGE: Native Antibodies (EU)
BGE: Native Antibodies (UK)
BGE: Native Antibodies (USA)

Diluent: Native Antibodies

Diluent: Native Antibodies (EU)
Diluent: Native Antibodies (UK)
Diluent: Native Antibodies (USA)

BGE: Oligos

BGE: Oligos (EU)
BGE: Oligos (UK)
BGE: Oligos (USA)

Diluent: Oligos

Diluent: Oligos (EU)
Diluent: Oligos (UK)
Diluent: Oligos (USA)

BGE: Peptides

BGE: Peptides (EU)
BGE: Peptides (UK)
BGE: Peptides (USA)

Diluent: Peptides

Diluent: Peptides (EU)
Diluent: Peptides (UK)
Diluent: Peptides (USA)

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Wistia video thumbnail
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                    [post_content] => 

This webinar consists of two parts: Dr. Adi Kulkarni begins by providing a brief introduction to the ZipChip CE-MS technology, highlighting its features and benefits, followed by Dr. Josh Smith from NIBRT, with a deep dive of the ZipChip CE-MS technology applied to the characterization of AAVs.

Commonly utilized liquid chromatography-mass spectrometry (LC-MS) characterization strategies are time consuming and sample intensive. Capillary electrophoresis (CE) is often adapted as an orthogonal alternative for biotherapeutic characterization because of its speed and sensitivity. However, the utility of CE is generally limited by poor compatibility with MS. ZipChip/MS overcomes these incompatibility issues enabling rapid in-depth characterization of biotherapeutics.

In the first part of the webinar, Dr. Kulkarni will provide an overview of the ZipChip/MS technology and explain the fundamental concepts behind how ZipChip/MS works. In the second part of the webinar, Dr. Smith will present results obtained by applying the ZipChip/MS to the characterization of Adeno-Associated Virus (AAV) capsid proteins. AAVs are viral vectors increasingly investigated for their clinical potential as delivery systems of gene therapy targets. Characterization of intact AAV capsids, their viral proteins (VPs), and their post-translational modifications (PTMs) is critical to monitoring and ensuring product quality and efficacy.  

Dr. Smith will demonstrate how microchip CE-MS can be applied to the characterization of AAVs, specifically VP characterization and peptide mapping. Performed across a spectrum of AAV serotypes, we discuss how this platform quickly provides crucial information related to the characteristics of AAVs including the presence of VP proteoforms and PTMs.

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[post_title] => In-Depth Adeno-Associated Virus Characterization with Microchip Capillary Electrophoresis-Mass Spectrometry [post_excerpt] => This webinar consists of two parts: Dr. Adi Kulkarni from 908 Devices begins by providing a brief introduction to the ZipChip CE-MS technology, highlighting its features and benefits. In part two, Dr. Josh Smith from NIBRT, demonstrates how microchip CE-MS can be applied to the characterization of AAVs, specifically VP characterization and peptide mapping. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-in-depth-adeno-associated-virus-characterization [to_ping] => [pinged] => [post_modified] => 2024-05-24 00:08:35 [post_modified_gmt] => 2024-05-24 04:08:35 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=23016 [menu_order] => 185 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [1] => WP_Post Object ( [ID] => 8043 [post_author] => 29 [post_date] => 2023-05-01 14:02:59 [post_date_gmt] => 2023-05-01 18:02:59 [post_content] =>

Peptide mapping is considered the gold standard for the analysis of biopharmaceuticals. Classical analysis is performed through long workflows involving extensive sample handling and long liquid chromatography (LC-MS) analysis times.

The distinct advantages of short analysis times and unique separation mechanisms makes Capillary Electrophoresis (CE-MS) an attractive alternative for the peptide mapping analysis. Nevertheless, most existing sample preparation protocols are either incompatible with CE-MS analysis or require additional steps for salt removal that may increase sample handling.

  • An overview of the ZipChip Microchip CE-MS technology and its typical application space
  • How to optimize CE-MS compatible sample preparation using a total workflow length of 2 hours
    • Data analysis will provide full sequence coverage and accurate quantitation of product quality attributes
    • Wide applicability of the workflow to different IgG subclasses will be demonstrated
  • A panel discussion (Q&A) at the end
  • Microchip CE-MS technology, its capabilities, and typical application space
  • How CE-MS technology can enhance peptide mapping throughput
  • Coupling peptide mapping sample preparation of monoclonal antibodies to CE-MS analysis
  • CE-MS analysis provides robust determination of quality attributes in less than 15 minutes

Dr. Sara Carillo completed her Ph.D. in Chemical Sciences in 2013 at the University of Naples “Federico II”. Under the guidance of Prof. Corsaro, she focused on the structural characterisation of polysaccharides and glyco-conjugates from Gram-negative bacteria via NMR and mass spectrometry techniques, focusing on the immunological properties and potentials of extremophiles endotoxins. After a period at the University College of Dublin, in 2015 she joined Dr. Jonathan Bones’s research group in NIBRT working on understanding of the effects of extractables and leachables from single-use bioreactors on CHO cells N-glycome and produced monoclonal antibodies. She is now working in NIBRT as Bioanalytical Research Lead and is mainly interested in the development of new analytical approaches to ensure a deeper and easier understanding of biomanufacturing processes and biopharmaceuticals’ structural complexity.

Adi Kulkarni is a Principal Scientist. Adi has extensive experience in applying CE-MS in biopharma characterization workflows using different mass spec platforms. He has developed novel applications based on the ZipChip technology and managing strategic collaborations and partnerships. Prior to working at 908 Devices, Adi was an applications scientist at Bruker Daltonics where he worked on Bruker’s Q-TOF line of products. He received his Ph.D. at the University of Massachusetts in 2011 where he developed novel methodologies for the synthesis of nitrogen-containing heterocycles. After his Ph.D., Adi was a postdoctoral fellow at University of Southern California’s Loker Hydrocarbon Research Institute where he discovered new reagents for applications in organofluorine chemistry. He has authored 18 peer-reviewed articles.

Jonathan Bones is the principal investigator of the Characterisation and Comparability Laboratory at NIBRT. An analytical chemist by training, Jonathan’s research group specializes in the development and application of analytical solutions for problems associated with the manufacture and characterization of biopharmaceuticals. This includes recombinant proteins, monoclonal antibodies, and advanced therapies like AAV based gene therapy, cell therapy, and mRNA using their core excellence in analytical separations coupled to mass spectrometry.

Erin Redman is a Principal Scientist in the Research and Development division of 908 Devices. She joined the company in 2016 and has worked to develop protein characterization assays using the ZipChip technology and further the innovation microfluidic CE separations coupled to mass spectrometry. Prior to starting her career at 908 Devices, she received her Ph.D. under the direction of Professor J. Michael Ramsey at the University of North Carolina at Chapel Hill. There she developed microfluidic technology for interfacing high resolution separations with electrospray mass spectrometry for the characterization of intact biotherapeutic proteins and proteins from biofluids.

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[post_title] => Direct Microchip CE/MS Peptide Mapping Workflow for the Analysis of mAb CQAs [post_excerpt] => Microchip CE-MS technology, its capabilities, and typical application space. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-webinar-ls-zc-wb-0017 [to_ping] => [pinged] => [post_modified] => 2024-08-01 13:52:43 [post_modified_gmt] => 2024-08-01 17:52:43 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=8043 [menu_order] => 188 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [2] => WP_Post Object ( [ID] => 5044 [post_author] => 38 [post_date] => 2021-12-09 15:13:01 [post_date_gmt] => 2021-12-09 20:13:01 [post_content] =>
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[post_title] => Simplifying Oligonucleotide Characterization with Microchip CE-MS [post_excerpt] => In this on-demand webinar, we present a microfluidic capillary zone electrophoresis-based separation coupled with HRAM mass-spectrometry for rapid characterization of oligos in positive ionisation mode. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => simplifying-oligonucleotide-characterization-with-microchip-ce-ms [to_ping] => [pinged] => [post_modified] => 2024-10-02 06:15:56 [post_modified_gmt] => 2024-10-02 10:15:56 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=5044 [menu_order] => 284 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [3] => WP_Post Object ( [ID] => 1167 [post_author] => 38 [post_date] => 2020-12-17 21:19:38 [post_date_gmt] => 2020-12-18 02:19:38 [post_content] =>
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[post_title] => Fast Therapeutic Protein Characterization [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-webinar-tmo-fast-therapeutic-protein-characterization [to_ping] => [pinged] => [post_modified] => 2024-10-01 11:11:12 [post_modified_gmt] => 2024-10-01 15:11:12 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/resource/zipchip_webinar_tmo_fast-therapeutic-protein-characterization/ [menu_order] => 424 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) ) [post_count] => 4 [current_post] => -1 [before_loop] => 1 [in_the_loop] => [post] => WP_Post Object ( [ID] => 23016 [post_author] => 29 [post_date] => 2023-12-20 10:11:09 [post_date_gmt] => 2023-12-20 15:11:09 [post_content] =>

This webinar consists of two parts: Dr. Adi Kulkarni begins by providing a brief introduction to the ZipChip CE-MS technology, highlighting its features and benefits, followed by Dr. Josh Smith from NIBRT, with a deep dive of the ZipChip CE-MS technology applied to the characterization of AAVs.

Commonly utilized liquid chromatography-mass spectrometry (LC-MS) characterization strategies are time consuming and sample intensive. Capillary electrophoresis (CE) is often adapted as an orthogonal alternative for biotherapeutic characterization because of its speed and sensitivity. However, the utility of CE is generally limited by poor compatibility with MS. ZipChip/MS overcomes these incompatibility issues enabling rapid in-depth characterization of biotherapeutics.

In the first part of the webinar, Dr. Kulkarni will provide an overview of the ZipChip/MS technology and explain the fundamental concepts behind how ZipChip/MS works. In the second part of the webinar, Dr. Smith will present results obtained by applying the ZipChip/MS to the characterization of Adeno-Associated Virus (AAV) capsid proteins. AAVs are viral vectors increasingly investigated for their clinical potential as delivery systems of gene therapy targets. Characterization of intact AAV capsids, their viral proteins (VPs), and their post-translational modifications (PTMs) is critical to monitoring and ensuring product quality and efficacy.  

Dr. Smith will demonstrate how microchip CE-MS can be applied to the characterization of AAVs, specifically VP characterization and peptide mapping. Performed across a spectrum of AAV serotypes, we discuss how this platform quickly provides crucial information related to the characteristics of AAVs including the presence of VP proteoforms and PTMs.

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[post_title] => In-Depth Adeno-Associated Virus Characterization with Microchip Capillary Electrophoresis-Mass Spectrometry [post_excerpt] => This webinar consists of two parts: Dr. Adi Kulkarni from 908 Devices begins by providing a brief introduction to the ZipChip CE-MS technology, highlighting its features and benefits. In part two, Dr. Josh Smith from NIBRT, demonstrates how microchip CE-MS can be applied to the characterization of AAVs, specifically VP characterization and peptide mapping. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-in-depth-adeno-associated-virus-characterization [to_ping] => [pinged] => [post_modified] => 2024-05-24 00:08:35 [post_modified_gmt] => 2024-05-24 04:08:35 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=23016 [menu_order] => 185 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [comment_count] => 0 [current_comment] => -1 [found_posts] => 4 [max_num_pages] => 0 [max_num_comment_pages] => 0 [is_single] => [is_preview] => [is_page] => [is_archive] => 1 [is_date] => [is_year] => [is_month] => [is_day] => [is_time] => [is_author] => [is_category] => [is_tag] => [is_tax] => 1 [is_search] => [is_feed] => [is_comment_feed] => [is_trackback] => [is_home] => [is_privacy_policy] => [is_404] => [is_embed] => [is_paged] => [is_admin] => [is_attachment] => [is_singular] => [is_robots] => [is_favicon] => [is_posts_page] => [is_post_type_archive] => 1 [query_vars_hash:WP_Query:private] => 6a3a11a59666251ed44c74bb625f0256 [query_vars_changed:WP_Query:private] => 1 [thumbnails_cached] => [allow_query_attachment_by_filename:protected] => [stopwords:WP_Query:private] => [compat_fields:WP_Query:private] => Array ( [0] => query_vars_hash [1] => query_vars_changed ) [compat_methods:WP_Query:private] => Array ( [0] => init_query_flags [1] => parse_tax_query ) )
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