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=> [post_title] => Microchip CE/MS Peptide Mapping Workflow with Trypsin Digestion for the Analysis of Critical Quality Attributes of Monoclonal Antibodies [post_excerpt] => Peptide mapping is considered the gold standard for the analysis of biopharmaceuticals, including monoclonal antibodies. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-appnote-ls-zc-an-0020 [to_ping] => [pinged] => [post_modified] => 2024-08-01 13:35:51 [post_modified_gmt] => 2024-08-01 17:35:51 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=8033 [menu_order] => 100 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [1] => WP_Post Object ( [ID] => 4835 [post_author] => 29 [post_date] => 2021-10-29 12:13:11 [post_date_gmt] => 2021-10-29 16:13:11 [post_content] => [post_title] => Rapid characterization of oligonucleotides using microfluidic CE-MS by ZipChip [post_excerpt] => This application note presents a CE-MS analysis of oligos by a ZipChip coupled to a QExactive HF mass spectrometer. This positive ESI mode method utilizes a MS-friendly background electrolyte (BGE), that eliminates the use of ion-paring agents such as hexafluoro isopropanol or alkyl amines. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-app-note-oligo-analysis [to_ping] => [pinged] => [post_modified] => 2024-08-01 13:24:15 [post_modified_gmt] => 2024-08-01 17:24:15 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=4835 [menu_order] => 292 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) ) [post_count] => 2 [current_post] => -1 [before_loop] => 1 [in_the_loop] => [post] => WP_Post Object ( [ID] => 8033 [post_author] => 29 [post_date] => 2023-05-01 11:52:32 [post_date_gmt] => 2023-05-01 15:52:32 [post_content] => [post_title] => Microchip CE/MS Peptide Mapping Workflow with Trypsin Digestion for the Analysis of Critical Quality Attributes of Monoclonal Antibodies [post_excerpt] => Peptide mapping is considered the gold standard for the analysis of biopharmaceuticals, including monoclonal antibodies. 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Application Notes & Snapshots
Microchip CE/MS Peptide Mapping Workflow with Trypsin Digestion for the Analysis of Critical Quality Attributes of Monoclonal Antibodies
Peptide mapping is considered the gold standard for the analysis of biopharmaceuticals, including monoclonal antibodies.
Rapid characterization of oligonucleotides using microfluidic CE-MS by ZipChip
This application note presents a CE-MS analysis of oligos by a ZipChip coupled to a QExactive HF mass spectrometer. This positive ESI mode method utilizes a MS-friendly background electrolyte (BGE), that eliminates the use of ion-paring agents such as hexafluoro isopropanol or alkyl amines.
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CONSUMABLES GUIDE
From complex biologics to small molecules,
ZipChip has an assay kit for you.[post_title] => ZipChip Consumables Guide [post_excerpt] => Learn how to take the guesswork out of method development with ZipChip. Breeze through your selection in three quick steps. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-consumables-guide [to_ping] => [pinged] => [post_modified] => 2024-08-30 12:00:02 [post_modified_gmt] => 2024-08-30 16:00:02 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=5077 [menu_order] => 279 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [1] => WP_Post Object ( [ID] => 4837 [post_author] => 36 [post_date] => 2021-10-29 12:17:00 [post_date_gmt] => 2021-10-29 16:17:00 [post_content] => Take the Guesswork Out of Method Development
3 Easy Steps to Selection
1. Application
2. Chip
3. Kit
Assay kits come with background electrolyte and sample diluent that’s optimized for each application. Just put the bottle of background electrolyte in the autosampler, prep your samples with the sample diluent and you’re off.
1. The Application
From large to small molecule, ZipChip’s got you covered.
Protein Characterization:
ZipChip is a one-stop shop for CQA’s—from charge variant analysis to peptide mapping. You’ll get high-resolution separation coupled to MS with simple, streamlined workflows.Metabolomics: Quickly analyze small polar analytes from a variety of matrices, or monitor dynamic levels of metabolites in real time. Sample prep is quick and easy and results are ready in minutes.
Oligonucleotides: The characterization of nucleic acid-based drugs like oligonucleotides isn’t easy. ZipChip can offer some advantage over LC separations, particularly variations due to charge or shape.
Small Molecules: Analysis is easy and fast with ZipChip. Simply dilute and shoot; there’s no labeling or derivatization. Small polar analyte assays take as little as 2 minutes. Add internal standards for full quantitation on a variety of analytes.
2. The Chips
Choose from a range of chip options to optimize your workflow. Each chip can be used up to 125 injections.
HS: High Speed chip enables fastest separations. Matching BGE kits: Intact Antibody, Peptides, or Metabolites.
HR: High Resolution chip for applications demanding maximum separation capacities. Matching BGE kits: Intact Antibody, Peptides, or Metabolites.
HSN: High Speed chip for fast native protein analysis. Matching BGE kits: Native Antibodies or Charge Variant Analysis.
HRN: High Resolution chip with ultimate separation for native protein analysis. Matching BGE kits: Native Antibodies, or Charge Variant Analysis.
HSB: High Speed Bare Glass chip for high throughput separations. For example: fast oligonucleotides analysis. Matching BGE kit example: Oligos BGE.
HRB: High Resolution Bare Glass chip for high resolution separation of negatively charged analytes such as oligonucleotides. Matching BGE kit example: Oligos BGE.3. BGE Kits
Background electrolyte (BGE) and sample diluent reduce preparation time for your samples. With ZipChip you just dilute and shoot!
Charge Variant Analysis: Formulated to provide excellent charge variant separations and MS sensitivity for antibodies and other proteins on all mass spectrometers.
Native Antibodies: Excellent charge variant separation of antibodies and other constructs to generate native spectra.
Peptides: Fast, efficient separations of protein digests and peptides utilizing low pH.
Metabolites: Streamlined sample prep and high-speed analysis for small molecules in denaturing conditions at low pH.
Oligonucleotides: Rapid analysis of oligonucleotides without the use of ion-pairing agents.Ordering Information
ZipChip Assay Kits* Part Number Samples Per Kit Charge Variant Analysis Kit 850-00052 625 Native Antibody Assay 850-00048 625 Metabolites Assat 850-00033 625 Peptide Assay 850-00034 625 Oligos Assay 850-00066 625 Oligos Starter 850-00067 250 Tests *All kits contain BGE and diluent, and chips need to be ordered separately. The exception is the oligos starter kit which contains BGE, Diluent, chips and a standard.
Chips Part Number Chips Per Box Injections Per Chip HS 810-00195 5 125 HR 810-00194 5 125 HSN 810-00230 5 125 HRN 810-00227 5 125 HSB 810-00237 5 125 HRB 810-00239 5 125 HASSLE FREE
Oligo characterization with ZipChip
The newly released ZipChip® oligos kit provides a simple, sensitive, dilute-and-shoot method for rapid analysis of oligonucleotides, applicable to various sizes and modifications.
Benefits:
- High-resolution accurate mass identification and quantitation
with CE-MS- No ion-pairing agents; no instrument contamination
- Orthogonal to LC-based separation
- Analysis in minutes with simple sample prep
Part Name Contents Samples per kit Part No. ZipChip Oligos Starter Kit Oligos Background electrolyte (BGE) x 2
Diluent x 2
HRB Chip x 2
Oligo standard250 850-00067 ZipChip Oligos Kit Oligos BGE x 5
Diluent x 5625 850-00066 HSB Chip High Speed Bare Glass Chip (HSB) x 5 625 810-00237 HRB Chip High Resolution Bare Glass Chip (HRB) x 5 625 810-00239 ZipChip Oligos Standard 20 mer DNA Primer - 445-02281 [post_title] => Hassle-Free Oligo Characterization with ZipChip [post_excerpt] => The newly released ZipChip® oligos kit provides a simple, sensitive, dilute-and-shoot method for rapid analysis of oligonucleotides, applicable to various sizes and modifications. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-flyer-oligo-kit [to_ping] => [pinged] => [post_modified] => 2024-08-21 14:17:49 [post_modified_gmt] => 2024-08-21 18:17:49 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=4837 [menu_order] => 291 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [2] => WP_Post Object ( [ID] => 827 [post_author] => 36 [post_date] => 2018-06-04 04:28:30 [post_date_gmt] => 2018-06-04 08:28:30 [post_content] => Rapid Characterization of Oligos
Separation of Modified ssRNAs
Electropherogram showing separation of biotinylated, glycosylated and phosphorylated 20 mers from the corresponding unmodified 20 mer RNA standard
Rapid characterization of ssRNA with no method development
Base Peak Electropherogram for 20mer unmodified ssRNA standards (left) along with the raw mass spectrum (right).
ZipChip leverages microfluidic-based Capillary Electrophoresis (CE) and electrospray ionization technology to simplify sample preparation, perform high-resolution separations with reduced analysis times, and directly introduce samples into a Thermo Fisher Scientific, Bruker, or Sciex mass spectrometer. Achieve faster results with the ZipChip CE-MS device.
CE-MS DEVICE
ZipChip
ACCELERATE YOUR MS CHARACTERIZATION
Rapid protein characterization and metabolomic analysis with microfluidic technology for highly efficient separations of complex samples.
BIOLOGICAL CHARACTERIZATION
IN MINUTES
GIVE YOUR MASS SPEC SOME ZIP
The ZipChip® platform prepares and separates a wide range of biological samples, then electrosprays them into your mass spec for analysis. Just clip it on. The process takes as little as a few minutes, and it results in better separation quality than most LCs in a fraction of the time. Simple workflows and multiple kit options cover a host of biotherapeutic, metabolomic, and proteomic applications.
EASY ON SAMPLES
Now the kicker. ZipChip plays well with your mass spec and your proteins. Gentle sample prep reagents preserve structural integrity without denaturing or unfolding proteins for fully native mass spectra. No need to remove detergents or desalt. Negative and neutrals are trashed. Only positive analytes head out for cleaner mass spectra and more identified peaks.
EFFICIENCY WITH ADVANCED
MICROCHIP TECHNOLOGY
WITH ZIPCHIP YOU GET THE DATA FASTER, AND BECAUSE OF THAT YOU CAN MAKE QUICK DECISIONS
Associate scientist at Amgen
Easy as 1-2-3
Pick an application, a chip, and a kit. After simply loading the premixed background electrolyte and sample into the autosampler, pop in a ZipChip, and hit run. Use vials or 96-well plates.
It’s hands-off from there. Smart chips tell the system which method to use based on your preferences. Samples are automatically loaded, separated, and electrosprayed. Integrated software cues the mass spec to start analysis.Efficient Separations
Microfluidic technology integrates capillary electrophoresis (CE) and electrospray ionization (ESI) on the ZipChip. Junctions and dead volumes don’t exist so analytical quality stays high, and no injection bias improves repeatability.
1. Pick your application, kit, and chip
2. Simple sample prep
3. Load reagent and chip, then run
IT'S ALL IN THE CHIP
FROM LARGE TO SMALL MOLECULES,
ZIPCHIP’S GOT YOU COVERED
[post_title] => ZipChip: Accelerate Your MS Characterization [post_excerpt] => Rapid protein characterization and metabolomic analysis with microfluidic technology for highly efficient separations of complex samples. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-platform-brochure [to_ping] => [pinged] => [post_modified] => 2024-08-28 16:38:39 [post_modified_gmt] => 2024-08-28 20:38:39 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/resource/zipchip-platform-brochure/ [menu_order] => 329 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) ) [post_count] => 3 [current_post] => -1 [before_loop] => 1 [in_the_loop] => [post] => WP_Post Object ( [ID] => 5077 [post_author] => 36 [post_date] => 2021-12-17 09:39:33 [post_date_gmt] => 2021-12-17 14:39:33 [post_content] => Protein Characterization:
ZipChip is a one-stop shop for CQAs—with streamlined CE MS workflows for Charge Variant Analysis of basic and acidic species, Rapid Intact Mass Analysis, Subunit Analysis, and Peptide Mapping for deeper characterization.
Oligonucleotides:
The characterization of nucleic acid-based drugs like oligonucleotides isn’t easy. ZipChip can offer some advantage over LC separations, such as no ion-pairing agents to contaminate the instrument
Small Molecules
Analysis is easy and fast with ZipChip. Simply dilute and shoot; there’s no labeling or derivatization. Small polar analyte assays take as little as 2 minutes. Add internal standards for full quantitation on a variety of analytes.
Metabolomics:
Detect, identify and quantitate small polar analytes from a variety of matrices like cell lysates or growth media and monitor dynamic levels of metabolites in real time. Sample prep is quick and easy and results are ready in minutes.
ZipChip
CONSUMABLES GUIDE
From complex biologics to small molecules,
ZipChip has an assay kit for you.[post_title] => ZipChip Consumables Guide [post_excerpt] => Learn how to take the guesswork out of method development with ZipChip. Breeze through your selection in three quick steps. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-consumables-guide [to_ping] => [pinged] => [post_modified] => 2024-08-30 12:00:02 [post_modified_gmt] => 2024-08-30 16:00:02 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=5077 [menu_order] => 279 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [comment_count] => 0 [current_comment] => -1 [found_posts] => 3 [max_num_pages] => 0 [max_num_comment_pages] => 0 [is_single] => [is_preview] => [is_page] => [is_archive] => 1 [is_date] => [is_year] => [is_month] => [is_day] => [is_time] => [is_author] => [is_category] => [is_tag] => [is_tax] => 1 [is_search] => [is_feed] => [is_comment_feed] => [is_trackback] => [is_home] => [is_privacy_policy] => [is_404] => [is_embed] => [is_paged] => [is_admin] => [is_attachment] => [is_singular] => [is_robots] => [is_favicon] => [is_posts_page] => [is_post_type_archive] => 1 [query_vars_hash:WP_Query:private] => d8776f276181eb4a4ad23c0c389fb549 [query_vars_changed:WP_Query:private] => 1 [thumbnails_cached] => [allow_query_attachment_by_filename:protected] => [stopwords:WP_Query:private] => [compat_fields:WP_Query:private] => Array ( [0] => query_vars_hash [1] => query_vars_changed ) [compat_methods:WP_Query:private] => Array ( [0] => init_query_flags [1] => parse_tax_query ) ) Take the Guesswork Out of Method Development
3 Easy Steps to Selection
1. Application
2. Chip
3. Kit
Assay kits come with background electrolyte and sample diluent that’s optimized for each application. Just put the bottle of background electrolyte in the autosampler, prep your samples with the sample diluent and you’re off.
1. The Application
From large to small molecule, ZipChip’s got you covered.
Protein Characterization:
ZipChip is a one-stop shop for CQA’s—from charge variant analysis to peptide mapping. You’ll get high-resolution separation coupled to MS with simple, streamlined workflows.Metabolomics: Quickly analyze small polar analytes from a variety of matrices, or monitor dynamic levels of metabolites in real time. Sample prep is quick and easy and results are ready in minutes.
Oligonucleotides: The characterization of nucleic acid-based drugs like oligonucleotides isn’t easy. ZipChip can offer some advantage over LC separations, particularly variations due to charge or shape.
Small Molecules: Analysis is easy and fast with ZipChip. Simply dilute and shoot; there’s no labeling or derivatization. Small polar analyte assays take as little as 2 minutes. Add internal standards for full quantitation on a variety of analytes.
2. The Chips
Choose from a range of chip options to optimize your workflow. Each chip can be used up to 125 injections.
HS: High Speed chip enables fastest separations. Matching BGE kits: Intact Antibody, Peptides, or Metabolites.
HR: High Resolution chip for applications demanding maximum separation capacities. Matching BGE kits: Intact Antibody, Peptides, or Metabolites.
HSN: High Speed chip for fast native protein analysis. Matching BGE kits: Native Antibodies or Charge Variant Analysis.
HRN: High Resolution chip with ultimate separation for native protein analysis. Matching BGE kits: Native Antibodies, or Charge Variant Analysis.
HSB: High Speed Bare Glass chip for high throughput separations. For example: fast oligonucleotides analysis. Matching BGE kit example: Oligos BGE.
HRB: High Resolution Bare Glass chip for high resolution separation of negatively charged analytes such as oligonucleotides. Matching BGE kit example: Oligos BGE.3. BGE Kits
Background electrolyte (BGE) and sample diluent reduce preparation time for your samples. With ZipChip you just dilute and shoot!
Charge Variant Analysis: Formulated to provide excellent charge variant separations and MS sensitivity for antibodies and other proteins on all mass spectrometers.
Native Antibodies: Excellent charge variant separation of antibodies and other constructs to generate native spectra.
Peptides: Fast, efficient separations of protein digests and peptides utilizing low pH.
Metabolites: Streamlined sample prep and high-speed analysis for small molecules in denaturing conditions at low pH.
Oligonucleotides: Rapid analysis of oligonucleotides without the use of ion-pairing agents.Ordering Information
ZipChip Assay Kits* Part Number Samples Per Kit Charge Variant Analysis Kit 850-00052 625 Native Antibody Assay 850-00048 625 Metabolites Assat 850-00033 625 Peptide Assay 850-00034 625 Oligos Assay 850-00066 625 Oligos Starter 850-00067 250 Tests *All kits contain BGE and diluent, and chips need to be ordered separately. The exception is the oligos starter kit which contains BGE, Diluent, chips and a standard.
Chips Part Number Chips Per Box Injections Per Chip HS 810-00195 5 125 HR 810-00194 5 125 HSN 810-00230 5 125 HRN 810-00227 5 125 HSB 810-00237 5 125 HRB 810-00239 5 125
Brochures & Flyers
ZipChip Consumables Guide
Learn how to take the guesswork out of method development with ZipChip. Breeze through your selection in three quick steps.
Hassle-Free Oligo Characterization with ZipChip
The newly released ZipChip® oligos kit provides a simple, sensitive, dilute-and-shoot method for rapid analysis of oligonucleotides, applicable to
various sizes and modifications.
ZipChip: Accelerate Your MS Characterization
Rapid protein characterization and metabolomic analysis with microfluidic technology for highly efficient separations of complex samples.
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(8033,24537,23966,23828,23820,23798,23794,23785,5772,23016,8043,6117,6114,6111,6109,6018,5998,5906,7606,7602,5077,5044,4926,4837,4835,838,832,827,788,786,1121,744,767,862,1066,858,1064,844,841,1167,856,877,874,1001,972) AND ( wp_term_relationships.term_taxonomy_id IN (75) ) AND ((wp_posts.post_type = 'resource' AND (wp_posts.post_status = 'publish' OR wp_posts.post_status = 'acf-disabled'))) AND ( ( ( wpml_translations.language_code = 'en' OR 0 ) AND wp_posts.post_type IN ('post','page','attachment','wp_block','wp_template','wp_template_part','wp_navigation','popup','products','applications','resource' ) ) OR wp_posts.post_type NOT IN ('post','page','attachment','wp_block','wp_template','wp_template_part','wp_navigation','popup','products','applications','resource' ) ) GROUP BY wp_posts.ID ORDER BY wp_posts.menu_order ASC [posts] => Array ( [0] => WP_Post Object ( [ID] => 1001 [post_author] => 36 [post_date] => 2020-03-18 21:00:07 [post_date_gmt] => 2020-03-19 01:00:07 [post_content] =>[post_title] => Architecture of autoinhibited and active BRAF–MEK1–14-3-3 complexes [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => architecture-of-autoinhibited-and-active-braf-mek1-14-3-3-complexes-2 [to_ping] => [pinged] => [post_modified] => 2024-12-11 13:01:56 [post_modified_gmt] => 2024-12-11 18:01:56 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/resource/architecture-of-autoinhibited-and-active-braf-mek1-14-3-3-complexes-2/ [menu_order] => 501 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) ) [post_count] => 1 [current_post] => -1 [before_loop] => 1 [in_the_loop] => [post] => WP_Post Object ( [ID] => 1001 [post_author] => 36 [post_date] => 2020-03-18 21:00:07 [post_date_gmt] => 2020-03-19 01:00:07 [post_content] => Researchers at the Dana-Farber Cancer Institute and Harvard Medical School leveraged the ZipChip® in a combined structural biology-phosphoproteomic study to decipher key topological features and post-translational modifications on the kinase BRAF. Like most cellular proteins, BRAF does not work alone, but rather assembles with other proteins into ‘molecular machines’ which work in concert to orchestrate the cellular ‘circuitry’ required for normal physiology. However, genetic mutation can commandeer and re-direct the activity of these protein machines leading to human tumors. In fact, BRAF mutations contribute to a large number of different human cancers.
The rapid-fire, 10-minute injection-to-injection throughput provided by the ZipChip enabled the researchers to analyze hundreds of samples spanning different BRAF mutations, protein partners, and proteolytic enzymes.
Unfortunately, the myriad of structural features, interaction surfaces, and post translational modifications on BRAF and its associated proteins make it challenging to understand how the kinase functions in normal physiology and how genetic mutations hijack the kinase and drive human cancer.
The Dana-Farber team generated purified forms of normal and mutant BRAF in addition to its key protein partners typically found in cells, and then assembled these to produce detailed molecular structures by cryo-electron microscopy. In addition, the researchers digested small aliquots of these complexes with a combination of proteolytic enzymes and then used the ZipChip on a Thermo Q-Exactive HF mass spectrometer to identify phosphorylation sites on BRAF and its co-regulatory protein partners. The rapid-fire, 10-minute injection-to injection throughput
provided by the ZipChip enabled the researchers to analyze hundreds of samples spanning different BRAF mutations, protein partners, and proteolytic enzymes. The multitude of ‘targeted’ and ‘unbiased’ CE-MS/ MS data sets enabled the researchers to read-out phosphorylation stoichiometry at key sites on BRAF which control its kinase activity and interaction with other co-regulatory proteins.
The Team combined their proteomic and structural data to better understand how BRAF mutations disrupt its normal cellular function. These results provide important insight to guide development of new, precision drugs which may improve clinical outcomes for patients with BRAF driven cancers.
To view the full article published in Nature Magazine click here.
[post_title] => Architecture of autoinhibited and active BRAF–MEK1–14-3-3 complexes [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => architecture-of-autoinhibited-and-active-braf-mek1-14-3-3-complexes-2 [to_ping] => [pinged] => [post_modified] => 2024-12-11 13:01:56 [post_modified_gmt] => 2024-12-11 18:01:56 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/resource/architecture-of-autoinhibited-and-active-braf-mek1-14-3-3-complexes-2/ [menu_order] => 501 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [comment_count] => 0 [current_comment] => -1 [found_posts] => 1 [max_num_pages] => 0 [max_num_comment_pages] => 0 [is_single] => [is_preview] => [is_page] => [is_archive] => 1 [is_date] => [is_year] => [is_month] => [is_day] => [is_time] => [is_author] => [is_category] => [is_tag] => [is_tax] => 1 [is_search] => [is_feed] => [is_comment_feed] => [is_trackback] => [is_home] => [is_privacy_policy] => [is_404] => [is_embed] => [is_paged] => [is_admin] => [is_attachment] => [is_singular] => [is_robots] => [is_favicon] => [is_posts_page] => [is_post_type_archive] => 1 [query_vars_hash:WP_Query:private] => 68d63c7a358d4120e60e8751e2c9f2c7 [query_vars_changed:WP_Query:private] => 1 [thumbnails_cached] => [allow_query_attachment_by_filename:protected] => [stopwords:WP_Query:private] => [compat_fields:WP_Query:private] => Array ( [0] => query_vars_hash [1] => query_vars_changed ) [compat_methods:WP_Query:private] => Array ( [0] => init_query_flags [1] => parse_tax_query ) ) Researchers at the Dana-Farber Cancer Institute and Harvard Medical School leveraged the ZipChip® in a combined structural biology-phosphoproteomic study to decipher key topological features and post-translational modifications on the kinase BRAF. Like most cellular proteins, BRAF does not work alone, but rather assembles with other proteins into ‘molecular machines’ which work in concert to orchestrate the cellular ‘circuitry’ required for normal physiology. However, genetic mutation can commandeer and re-direct the activity of these protein machines leading to human tumors. In fact, BRAF mutations contribute to a large number of different human cancers.
The rapid-fire, 10-minute injection-to-injection throughput provided by the ZipChip enabled the researchers to analyze hundreds of samples spanning different BRAF mutations, protein partners, and proteolytic enzymes.
Unfortunately, the myriad of structural features, interaction surfaces, and post translational modifications on BRAF and its associated proteins make it challenging to understand how the kinase functions in normal physiology and how genetic mutations hijack the kinase and drive human cancer.
The Dana-Farber team generated purified forms of normal and mutant BRAF in addition to its key protein partners typically found in cells, and then assembled these to produce detailed molecular structures by cryo-electron microscopy. In addition, the researchers digested small aliquots of these complexes with a combination of proteolytic enzymes and then used the ZipChip on a Thermo Q-Exactive HF mass spectrometer to identify phosphorylation sites on BRAF and its co-regulatory protein partners. The rapid-fire, 10-minute injection-to injection throughput
provided by the ZipChip enabled the researchers to analyze hundreds of samples spanning different BRAF mutations, protein partners, and proteolytic enzymes. The multitude of ‘targeted’ and ‘unbiased’ CE-MS/ MS data sets enabled the researchers to read-out phosphorylation stoichiometry at key sites on BRAF which control its kinase activity and interaction with other co-regulatory proteins.
The Team combined their proteomic and structural data to better understand how BRAF mutations disrupt its normal cellular function. These results provide important insight to guide development of new, precision drugs which may improve clinical outcomes for patients with BRAF driven cancers.
To view the full article published in Nature Magazine click here.
Case Studies
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=> [post_title] => Microchip CE-MS Analysis of Nucleic Acids – From Characterization of Synthetic Oligonucleotides to Sequence Mapping of RNAs [post_excerpt] => This work will showcase how the ZipChip enables a simple and rapid workflow for the analysis of nucleic acids with noion-pairing reagents needed This poster was highlighted at the 2024 TIDES Conference. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => microchip-ce-ms-analysis-of-nucleic-acids-from-characterization-of-synthetic-oligonucleotides-to-sequence-mapping-of-rnas [to_ping] => [pinged] => [post_modified] => 2024-08-01 10:55:48 [post_modified_gmt] => 2024-08-01 14:55:48 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=24537 [menu_order] => 105 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [1] => WP_Post Object ( [ID] => 23966 [post_author] => 29 [post_date] => 2024-04-01 13:05:13 [post_date_gmt] => 2024-04-01 17:05:13 [post_content] => [post_title] => Rapid In-Depth Characterization of Biologics by Microchip CE-MS: mAbs, AAVs and Nucleic Acids [post_excerpt] => Microchip capillary electrophoresis (CE) coupled with mass spectrometry (MS) has revolutionized the characterization of therapeutic modalities in biopharmaceuticals. Here, we present the applications of an integrated workflow that couples the microfluidic CE system (ZipChip) with MS in characterization of biotherapeutics. This was poster A03 at BPS Europe 2024. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => rapid-in-depth-characterization-of-biologics [to_ping] => [pinged] => [post_modified] => 2024-05-07 16:06:30 [post_modified_gmt] => 2024-05-07 20:06:30 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=23966 [menu_order] => 108 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [2] => WP_Post Object ( [ID] => 23828 [post_author] => 32 [post_date] => 2024-03-25 10:10:07 [post_date_gmt] => 2024-03-25 14:10:07 [post_content] => [post_title] => 5’ mRNA Analysis By Microchip CE-MS Using an Internal Cleavage Motif for RNase H Digestion [post_excerpt] => The subject of this poster was the investigation of an internal DNA motif to produce a shorter digestion product while simultaneously maintaining the length of the probe at 25nt for hybridization specificity. The shorter oligo results in easier detection using Microchip CE-MS approach. This poster was presented at ASMS 2023 as poster THP575. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => 5-mrna-analysis-by-microchip-ce-ms [to_ping] => [pinged] => [post_modified] => 2024-03-26 14:32:16 [post_modified_gmt] => 2024-03-26 18:32:16 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=23828 [menu_order] => 111 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [3] => WP_Post Object ( [ID] => 23820 [post_author] => 15 [post_date] => 2024-03-25 09:27:20 [post_date_gmt] => 2024-03-25 13:27:20 [post_content] => [post_title] => Rapid Identification of Conjugation Sites in Antibody Drug Conjugates Using Microchip Capillary Electrophoresis Coupled with Mass Spectrometry [post_excerpt] => CE/MS method is optimized with NISTmAb digest as a reference control. Good MS/MS coverage of NISTmAb is obtained with narrow peak width and fast separation. This poster was presented as a collaboration with Bruker at ASMS 2023 as poster WP041. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => rapid-identification-of-conjugation-sites-in-antibody-drug [to_ping] => [pinged] => [post_modified] => 2024-03-25 09:28:04 [post_modified_gmt] => 2024-03-25 13:28:04 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=23820 [menu_order] => 114 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [4] => WP_Post Object ( [ID] => 23798 [post_author] => 15 [post_date] => 2024-03-21 11:02:11 [post_date_gmt] => 2024-03-21 15:02:11 [post_content] => [post_title] => Fast Screening and Characterization of Therapeutic Peptide by Online Capillary Electrophoresis - Mass Spectrometry (CE-MS) [post_excerpt] => Presenting a novel strategy for characterization of therapeutic peptides was developed based on microchip CE-MS and linkage-specific derivatization. This poster was presented in collaboration with Ningbo University at ASMS 2023 as poster TP544. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => fast-screening-and-characterization-of-therapeutic-peptide [to_ping] => [pinged] => [post_modified] => 2024-03-21 11:02:12 [post_modified_gmt] => 2024-03-21 15:02:12 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=23798 [menu_order] => 116 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [5] => WP_Post Object ( [ID] => 23794 [post_author] => 15 [post_date] => 2024-03-21 10:38:52 [post_date_gmt] => 2024-03-21 14:38:52 [post_content] => [post_title] => Determination of ATP, ADP, AMP, and Adenosine Levels by Microchip Capillary Electrophoresis Coupled with High Resolution Mass Spectrometry [post_excerpt] => In this study, we present a novel ZipChip coupled with a QEHF for adenosine nucleotide analysis. This poster was presented at ASMS 2023 as poster TP489. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => determination-of-atp-adp-amp-and-adenosine-levels [to_ping] => [pinged] => [post_modified] => 2024-08-01 14:18:43 [post_modified_gmt] => 2024-08-01 18:18:43 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=23794 [menu_order] => 118 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [6] => WP_Post Object ( [ID] => 23785 [post_author] => 15 [post_date] => 2024-03-21 10:15:48 [post_date_gmt] => 2024-03-21 14:15:48 [post_content] => [post_title] => Peptide Mapping Workflow for Direct Microchip CE-MS Analysis of Biopharmaceuticals [post_excerpt] => In this study we demonstrated the suitability of a modified digestion workflow for peptide mapping analysis via CE separation. This poster was presented at ASMS 2023 in collaboration with NIBRT. It was poster MP619. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => peptide-mapping-workflow-for-direct-microchip [to_ping] => [pinged] => [post_modified] => 2024-03-21 10:15:49 [post_modified_gmt] => 2024-03-21 14:15:49 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=23785 [menu_order] => 121 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [7] => WP_Post Object ( [ID] => 6117 [post_author] => 20 [post_date] => 2022-06-11 02:59:33 [post_date_gmt] => 2022-06-11 06:59:33 [post_content] => [post_title] => Bottom-Up Analysis of Progressively Larger RNAs by CZE-MS and CZE-MS/MS [post_excerpt] => In this poster from ASMS 2022, researchers from University of Connecticut and Bruker showed that capillary zone electrophoresis performed on a chip proved to be an effective alternative to liquid chromatography as a front-end separation system to analyze both pure samples and complex digestion mixtures of nucleic acids. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-poster-asms2022-uconn [to_ping] => [pinged] => [post_modified] => 2023-12-26 12:43:57 [post_modified_gmt] => 2023-12-26 17:43:57 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=6117 [menu_order] => 193 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [8] => WP_Post Object ( [ID] => 6114 [post_author] => 29 [post_date] => 2022-06-11 02:42:23 [post_date_gmt] => 2022-06-11 06:42:23 [post_content] => [post_title] => A Sensitive and High-Throughput Assay for Analyzing Peptide Exchanged MHCl by High-Resolution Native CZE-MS [post_excerpt] => In this poster from ASMS 2022, researchers from Genentech describe a sensitive and high-throughput assay using capillary-zone electrophoresis coupled to a high-resolution mass spectrometer for characterizing peptide-exchanged MHCI under native conditions. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-poster-asms2022-genentech [to_ping] => [pinged] => [post_modified] => 2024-06-26 15:34:54 [post_modified_gmt] => 2024-06-26 19:34:54 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=6114 [menu_order] => 195 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [9] => WP_Post Object ( [ID] => 6111 [post_author] => 20 [post_date] => 2022-06-11 02:25:34 [post_date_gmt] => 2022-06-11 06:25:34 [post_content] => [post_title] => CE-MS of Oligonucleotides using a ZipChip [post_excerpt] => This ASMS 2022 poster from Boehringer Ingelheim explores the use of ZipChip coupled to a high resolution mass spectrometer as an orthogonal method to chromatography for oligonucleotide analysis. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-poster-asms2022-boehringer [to_ping] => [pinged] => [post_modified] => 2023-12-26 12:39:52 [post_modified_gmt] => 2023-12-26 17:39:52 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=6111 [menu_order] => 198 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [10] => WP_Post Object ( [ID] => 6109 [post_author] => 15 [post_date] => 2022-06-11 02:14:51 [post_date_gmt] => 2022-06-11 06:14:51 [post_content] => [post_title] => Rapid Characterization of Oligos and Related Impurities Using Microchip CE-MS without Ion-pairing Reagents [post_excerpt] => This poster from ASMS 2022 describes a simple & fast method for oligonucleotide characterizations using a Microchip CE-MS platform. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-poster-asms2022-kulkarni [to_ping] => [pinged] => [post_modified] => 2024-08-01 14:18:58 [post_modified_gmt] => 2024-08-01 18:18:58 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=6109 [menu_order] => 199 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [11] => WP_Post Object ( [ID] => 5998 [post_author] => 20 [post_date] => 2022-05-09 12:26:20 [post_date_gmt] => 2022-05-09 16:26:20 [post_content] => [post_title] => A Microchip CE-MS Based End-to-End Solution for the Analysis of Synthetic Oligonucleotides [post_excerpt] => This poster showcases a workflow using microchip CE-MS that is coupled with a vendor-neutral informatics platform as a complete solution for the analysis of synthetic oligos. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => a-microchip-ce-ms-based-end-to-end-solution-for-the-analysis-of-synthetic-oligonucleotides [to_ping] => [pinged] => [post_modified] => 2023-12-26 12:35:39 [post_modified_gmt] => 2023-12-26 17:35:39 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=5998 [menu_order] => 218 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [12] => WP_Post Object ( [ID] => 4926 [post_author] => 20 [post_date] => 2021-11-05 18:34:01 [post_date_gmt] => 2021-11-05 22:34:01 [post_content] => [post_title] => Microchip CE-MS of Oligonucleotides: What Can It Separate? [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-poster-microchip-ce-ms-of-oligonucleotides-what-can-it-separate [to_ping] => [pinged] => [post_modified] => 2023-12-26 12:34:32 [post_modified_gmt] => 2023-12-26 17:34:32 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=4926 [menu_order] => 287 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [13] => WP_Post Object ( [ID] => 788 [post_author] => 20 [post_date] => 2017-10-05 18:10:30 [post_date_gmt] => 2017-10-05 22:10:30 [post_content] => [post_title] => Fast Chip-CEMS for Characterization of Protein/PTM Changes [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => poster-fast-chip-cems-characterization-proteinptm-changes [to_ping] => [pinged] => [post_modified] => 2023-12-26 12:20:09 [post_modified_gmt] => 2023-12-26 17:20:09 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/resource/poster-fast-chip-cems-characterization-proteinptm-changes/ [menu_order] => 370 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [14] => WP_Post Object ( [ID] => 786 [post_author] => 20 [post_date] => 2017-10-05 17:52:35 [post_date_gmt] => 2017-10-05 21:52:35 [post_content] => [post_title] => Combined Top-down and Bottom-up Proteomics [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => poster-combined-top-bottom-proteomics-using-capillary-electrophoresis-mass-spectrometry [to_ping] => [pinged] => [post_modified] => 2023-12-26 12:19:21 [post_modified_gmt] => 2023-12-26 17:19:21 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/resource/poster-combined-top-bottom-proteomics-using-capillary-electrophoresis-mass-spectrometry/ [menu_order] => 374 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [15] => WP_Post Object ( [ID] => 862 [post_author] => 29 [post_date] => 2018-08-15 03:34:24 [post_date_gmt] => 2018-08-15 07:34:24 [post_content] => [post_title] => Rapid, Multi-level Analysis of Complex Proteins [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => rapid-multi-level-analysis-of-complex-proteins-by-microchip-capillary-electrophoresis-esi-ms [to_ping] => [pinged] => [post_modified] => 2024-06-26 15:12:49 [post_modified_gmt] => 2024-06-26 19:12:49 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/resource/rapid-multi-level-analysis-of-complex-proteins-by-microchip-capillary-electrophoresis-esi-ms/ [menu_order] => 411 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [16] => WP_Post Object ( [ID] => 1066 [post_author] => 20 [post_date] 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Posters
Microchip CE-MS Analysis of Nucleic Acids – From Characterization of Synthetic Oligonucleotides to Sequence Mapping of RNAs
This work will showcase how the ZipChip enables a simple and rapid workflow for the analysis of nucleic acids with noion-pairing reagents needed This poster was highlighted at the 2024 TIDES Conference.
Rapid In-Depth Characterization of Biologics by Microchip CE-MS: mAbs, AAVs and Nucleic Acids
Microchip capillary electrophoresis (CE) coupled with mass spectrometry (MS) has revolutionized the characterization of therapeutic modalities in biopharmaceuticals. Here, we present the applications of an integrated workflow that couples the microfluidic CE system (ZipChip) with MS in characterization of biotherapeutics. This was poster A03 at BPS Europe 2024.
5’ mRNA Analysis By Microchip CE-MS Using an Internal Cleavage Motif for RNase H Digestion
The subject of this poster was the investigation of an internal DNA motif to produce a shorter digestion product while simultaneously maintaining the length of the probe at 25nt for hybridization specificity. The shorter oligo results in easier detection using Microchip CE-MS approach. This poster was presented at ASMS 2023 as poster THP575.
Rapid Identification of Conjugation Sites in Antibody Drug Conjugates Using Microchip Capillary Electrophoresis Coupled with Mass Spectrometry
CE/MS method is optimized with NISTmAb digest as a reference control. Good MS/MS coverage of NISTmAb is obtained with narrow peak width and fast separation. This poster was presented as a collaboration with Bruker at ASMS 2023 as poster WP041.
Fast Screening and Characterization of Therapeutic Peptide by Online Capillary Electrophoresis – Mass Spectrometry (CE-MS)
Presenting a novel strategy for characterization of therapeutic peptides was developed based on microchip CE-MS and linkage-specific derivatization. This poster was presented in collaboration with Ningbo University at ASMS 2023 as poster TP544.
Determination of ATP, ADP, AMP, and Adenosine Levels by Microchip Capillary Electrophoresis Coupled with High Resolution Mass Spectrometry
In this study, we present a novel ZipChip coupled with a QEHF for adenosine nucleotide analysis. This poster was presented at ASMS 2023 as poster TP489.
Peptide Mapping Workflow for Direct Microchip CE-MS Analysis of Biopharmaceuticals
In this study we demonstrated the suitability of a modified digestion workflow for peptide mapping analysis via CE separation. This poster was presented at ASMS 2023 in collaboration with NIBRT. It was poster MP619.
Bottom-Up Analysis of Progressively Larger RNAs by CZE-MS and CZE-MS/MS
In this poster from ASMS 2022, researchers from University of Connecticut and Bruker showed that capillary zone electrophoresis performed on a chip proved to be an effective alternative to liquid chromatography as a front-end separation system to analyze both pure samples and complex digestion mixtures of nucleic acids.
A Sensitive and High-Throughput Assay for Analyzing Peptide Exchanged MHCl by High-Resolution Native CZE-MS
In this poster from ASMS 2022, researchers from Genentech describe a sensitive and high-throughput assay using capillary-zone electrophoresis coupled to a high-resolution mass spectrometer for characterizing peptide-exchanged MHCI under native conditions.
CE-MS of Oligonucleotides using a ZipChip
This ASMS 2022 poster from Boehringer Ingelheim explores the use of ZipChip coupled to a high resolution mass spectrometer as an orthogonal method to chromatography for oligonucleotide analysis.
Rapid Characterization of Oligos and Related Impurities Using Microchip CE-MS without Ion-pairing Reagents
This poster from ASMS 2022 describes a simple & fast method for oligonucleotide characterizations using a Microchip CE-MS platform.
A Microchip CE-MS Based End-to-End Solution for the Analysis of Synthetic Oligonucleotides
This poster showcases a workflow using microchip CE-MS that is coupled with a vendor-neutral informatics platform
as a complete solution for the analysis of synthetic oligos.
Microchip CE-MS of Oligonucleotides: What Can It Separate?
Fast Chip-CEMS for Characterization of Protein/PTM Changes
Combined Top-down and Bottom-up Proteomics
Rapid, Multi-level Analysis of Complex Proteins
Oligonucleotide Analysis via Microfluidic CE-MS
Advancing mAb Characterization with Microchip CE-MS Couples to a PASEF Enabled QTOF
Characterization of Protein/PTM Changes
NIBRT Presents ZipChip for Native Charge Heterogeneity
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Published Articles
Characterization of a SRPK1/2 Inhibitor Complex
Characterizing Covalent CDK Inhibitor Complexes
Rapid Fingerprinting of a Highly Glycosylated Fusion Protein
Monitoring Glycosylation Profile and Protein Titer in Cell Culture Samples
Characterizing Antiviral Protein-Inhibitor Complexes
Characterization of NIST mAb Proteoforms and Glycoforms
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the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.
Deoxyribonucleic Acid, Unmodified
SDS Deoxyribonucleic Acid, Unmodified
Interface Tray Grease
Standard: Amino Acids
SDS Standard: Amino Acids (EU)
SDS Standard: Amino Acids (UK)
SDS Standard: Amino Acids (USA)Additive: Charge Variant Analysis
SDS Additive: Charge Variant Analysis (EU)
SDS Additive: Charge Variant Analysis (UK)
SDS Additive: Charge Variant Analysis (USA)BGE: Charge Variant Analysis
BGE: Charge Variant Analysis (EU)
BGE: Charge Variant Analysis (UK)
BGE: Charge Variant Analysis (USA)Diluent: Charge Variant Analysis
Diluent: Charge Variant Analysis (EU)
Diluent: Charge Variant Analysis (UK)
Diluent: Charge Variant Analysis (USA)BGE: Intact Antibodies
BGE: Intact Antibodies (EU)
BGE: Intact Antibodies (UK)
BGE: Intact Antibodies (USA)Diluent: Intact Antibodies
Diluent: Intact Antibodies (EU)
Diluent: Intact Antibodies (UK)
Diluent: Intact Antibodies (USA)Acid: Metabolites
Acid: Metabolites (EU)
Acid: Metabolites (UK)
Acid: Metabolites (USA)BGE: Metabolites
BGE: Metabolites (EU)
BGE: Metabolites (UK)
BGE: Metabolites (USA)Diluent: Metabolites
Diluent: Metabolites (EU)
Diluent: Metabolites (UK)
Diluent: Metabolites (USA)BGE: Native Antibodies
BGE: Native Antibodies (EU)
BGE: Native Antibodies (UK)
BGE: Native Antibodies (USA)Diluent: Native Antibodies
Diluent: Native Antibodies (EU)
Diluent: Native Antibodies (UK)
Diluent: Native Antibodies (USA)BGE: Oligos
BGE: Oligos (EU)
BGE: Oligos (UK)
BGE: Oligos (USA)Diluent: Oligos
Diluent: Oligos (EU)
Diluent: Oligos (UK)
Diluent: Oligos (USA)BGE: Peptides
BGE: Peptides (EU)
BGE: Peptides (UK)
BGE: Peptides (USA)Diluent: Peptides
Diluent: Peptides (EU)
[post_title] => SDS - ZipChip [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => sds-zipchip [to_ping] => [pinged] => [post_modified] => 2024-11-15 14:46:57 [post_modified_gmt] => 2024-11-15 19:46:57 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=5906 [menu_order] => 230 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) ) [post_count] => 1 [current_post] => -1 [before_loop] => 1 [in_the_loop] => [post] => WP_Post Object ( [ID] => 5906 [post_author] => 29 [post_date] => 2022-04-14 12:53:24 [post_date_gmt] => 2022-04-14 16:53:24 [post_content] =>
Diluent: Peptides (UK)
Diluent: Peptides (USA)Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.
Deoxyribonucleic Acid, Unmodified
SDS Deoxyribonucleic Acid, Unmodified
Interface Tray Grease
Standard: Amino Acids
SDS Standard: Amino Acids (EU)
SDS Standard: Amino Acids (UK)
SDS Standard: Amino Acids (USA)Additive: Charge Variant Analysis
SDS Additive: Charge Variant Analysis (EU)
SDS Additive: Charge Variant Analysis (UK)
SDS Additive: Charge Variant Analysis (USA)BGE: Charge Variant Analysis
BGE: Charge Variant Analysis (EU)
BGE: Charge Variant Analysis (UK)
BGE: Charge Variant Analysis (USA)Diluent: Charge Variant Analysis
Diluent: Charge Variant Analysis (EU)
Diluent: Charge Variant Analysis (UK)
Diluent: Charge Variant Analysis (USA)BGE: Intact Antibodies
BGE: Intact Antibodies (EU)
BGE: Intact Antibodies (UK)
BGE: Intact Antibodies (USA)Diluent: Intact Antibodies
Diluent: Intact Antibodies (EU)
Diluent: Intact Antibodies (UK)
Diluent: Intact Antibodies (USA)Acid: Metabolites
Acid: Metabolites (EU)
Acid: Metabolites (UK)
Acid: Metabolites (USA)BGE: Metabolites
BGE: Metabolites (EU)
BGE: Metabolites (UK)
BGE: Metabolites (USA)Diluent: Metabolites
Diluent: Metabolites (EU)
Diluent: Metabolites (UK)
Diluent: Metabolites (USA)BGE: Native Antibodies
BGE: Native Antibodies (EU)
BGE: Native Antibodies (UK)
BGE: Native Antibodies (USA)Diluent: Native Antibodies
Diluent: Native Antibodies (EU)
Diluent: Native Antibodies (UK)
Diluent: Native Antibodies (USA)BGE: Oligos
BGE: Oligos (EU)
BGE: Oligos (UK)
BGE: Oligos (USA)Diluent: Oligos
Diluent: Oligos (EU)
Diluent: Oligos (UK)
Diluent: Oligos (USA)BGE: Peptides
BGE: Peptides (EU)
BGE: Peptides (UK)
BGE: Peptides (USA)Diluent: Peptides
Diluent: Peptides (EU)
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Diluent: Peptides (UK)
Diluent: Peptides (USA)
Safety Data Sheet (SDS)
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Just clip it on your mass spec and off you go! [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-hardware-spec-sheet [to_ping] => [pinged] => [post_modified] => 2024-12-02 16:13:23 [post_modified_gmt] => 2024-12-02 21:13:23 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/resource/zipchip-hardware-spec-sheet/ [menu_order] => 403 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) ) [post_count] => 1 [current_post] => -1 [before_loop] => 1 [in_the_loop] => [post] => WP_Post Object ( [ID] => 744 [post_author] => 36 [post_date] => 2017-03-27 14:39:06 [post_date_gmt] => 2017-03-27 18:39:06 [post_content] => ZipChip Interface
Capillary electrophoresis-mass spectrometry (CE-MS) has never been more convenient. The compact design of the ZipChip Interface seamlessly integrates all fluidics and electronics—just clip it on your mass spec and off you go!
Mass Spectrometer Compatibility Thermo Fisher Scientific: ZCI-Classic
- Exactive: Plus, Plus EMR
- Q Exactive: Biopharma, HF Biopharma, HF-X Biopharma, UHMR, Plus, HF,
- HF-X, Focus
- LTQ: XL, Orbitrap XL
ZC-Ti
- Orbitrap Astral
- Orbitrap Exploris: 120, 240, 240 Biopharma, 480, 480 Biopharma, MX,
MX Biopharma
- Orbitrap Tribrid: Fusion, Fusion Lumos, Eclipse, ID-X, IQ-X, Ascend
- TSQ: Triple Quad, Altis Triple Quad, Endura Triple QuadSCIEX: ZC-Si
- QTRAP: 4500, 6500+
- Triple Quad: 3500, 4500, 5500, 5500+, 6500, 6500+
- Triple TOF: 5600, 6600, 6600+BRUKER: ZC-Bi
- Compact
- Impact
- maXis II
- timsTOF: Pro 2, fleX, fleX MALDI-2, HT, MALDI PharmaPulse
- solariX
- scimaX
Specifications Software: - Standalone ZipChip acquisition software
- Integrates with your MS softwareDimensions: 7” W x 6.5” H x 11” D /18cm W x 17cm H x 28cm D Weight: 16 lbs / 7.3 Kg Power Requirements: 24V DC, 65W Laser Type: Class 1 Laser Product* *This equipment contains a 520 nm, 4.5 mW Class 3R laser diode with a beam divergence of 1.5 mrad but is classified as a Class 1 Laser Product as an end-use as evaluated to IEC 60825-1:2014. Avoid direct eye exposure to the beam and do not remove the laser for any other purpose.
Autosampler*
Achieve unattended sample runs from samples vials or 96-well plates. The platform allows you to choreograph sample runs using ZipChip software.
Specifications Sample Format: Vials or standard well plates (47 mm max height) Sample Capacity: Two sample trays that hold 48 vial racks or 96-well plates Sample Tray Cooling: - Built-in Peltier cooling
- Minimum: 4°C ± 2°C
- Maximum: ambient temperature –3°C
(Measured as air temperature in sample compartment for maximum air temperature 25°C and maximum humidity 80%)Dimensions: 11.75” W x 14.25” H x 22” D /30cm W x 36cm H x 57.5cm D Weight: 46.3 lbs / 21 kg Power Requirements: 100–240 VAC +/-10%, 50–60Hz, 200 VA *Required for ZipChip Interface, Autosampler version
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Capillary electrophoresis-mass spectrometry (CE-MS) has never been more convenient. The compact design of the ZipChip Interface seamlessly integrates all fluidics and electronics—just clip it on your mass spec and off you go!
Mass Spectrometer Compatibility Thermo Fisher Scientific: ZCI-Classic
- Exactive: Plus, Plus EMR
- Q Exactive: Biopharma, HF Biopharma, HF-X Biopharma, UHMR, Plus, HF,
- HF-X, Focus
- LTQ: XL, Orbitrap XL
ZC-Ti
- Orbitrap Astral
- Orbitrap Exploris: 120, 240, 240 Biopharma, 480, 480 Biopharma, MX,
MX Biopharma
- Orbitrap Tribrid: Fusion, Fusion Lumos, Eclipse, ID-X, IQ-X, Ascend
- TSQ: Triple Quad, Altis Triple Quad, Endura Triple QuadSCIEX: ZC-Si
- QTRAP: 4500, 6500+
- Triple Quad: 3500, 4500, 5500, 5500+, 6500, 6500+
- Triple TOF: 5600, 6600, 6600+BRUKER: ZC-Bi
- Compact
- Impact
- maXis II
- timsTOF: Pro 2, fleX, fleX MALDI-2, HT, MALDI PharmaPulse
- solariX
- scimaX
Specifications Software: - Standalone ZipChip acquisition software
- Integrates with your MS softwareDimensions: 7” W x 6.5” H x 11” D /18cm W x 17cm H x 28cm D Weight: 16 lbs / 7.3 Kg Power Requirements: 24V DC, 65W Laser Type: Class 1 Laser Product* *This equipment contains a 520 nm, 4.5 mW Class 3R laser diode with a beam divergence of 1.5 mrad but is classified as a Class 1 Laser Product as an end-use as evaluated to IEC 60825-1:2014. Avoid direct eye exposure to the beam and do not remove the laser for any other purpose.
Autosampler*
Achieve unattended sample runs from samples vials or 96-well plates. The platform allows you to choreograph sample runs using ZipChip software.
Specifications Sample Format: Vials or standard well plates (47 mm max height) Sample Capacity: Two sample trays that hold 48 vial racks or 96-well plates Sample Tray Cooling: - Built-in Peltier cooling
- Minimum: 4°C ± 2°C
- Maximum: ambient temperature –3°C
(Measured as air temperature in sample compartment for maximum air temperature 25°C and maximum humidity 80%)Dimensions: 11.75” W x 14.25” H x 22” D /30cm W x 36cm H x 57.5cm D Weight: 46.3 lbs / 21 kg Power Requirements: 100–240 VAC +/-10%, 50–60Hz, 200 VA *Required for ZipChip Interface, Autosampler version
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Tech Notes
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[post_excerpt] => Recorded at ASMS 2022, Dan Fabris from the University of Connecticut shares his experience with RNA characterization using ZipChip. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-video-dan-fabris-asms2022 [to_ping] => [pinged] => [post_modified] => 2024-09-05 14:40:57 [post_modified_gmt] => 2024-09-05 18:40:57 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=7606 [menu_order] => 233 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [1] => WP_Post Object ( [ID] => 7602 [post_author] => 29 [post_date] => 2023-01-24 17:19:20 [post_date_gmt] => 2023-01-24 22:19:20 [post_content] =>[post_title] => Interview: Oligonucleotide Analysis with ZipChip at Boehringer Ingelheim [post_excerpt] => Recorded at ASMS 2022, Vladimir Papov shares his experience with oligo analysis using ZipChip. 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Testimonials
Interview: The Key to Unsolved Insights into RNA Characterization?
Recorded at ASMS 2022, Dan Fabris from the University of Connecticut shares his experience with RNA characterization using ZipChip.
Interview: Oligonucleotide Analysis with ZipChip at Boehringer Ingelheim
Recorded at ASMS 2022, Vladimir Papov shares his experience with oligo analysis using ZipChip.
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This webinar consists of two parts: Dr. Adi Kulkarni begins by providing a brief introduction to the ZipChip CE-MS technology, highlighting its features and benefits, followed by Dr. Josh Smith from NIBRT, with a deep dive of the ZipChip CE-MS technology applied to the characterization of AAVs.
Commonly utilized liquid chromatography-mass spectrometry (LC-MS) characterization strategies are time consuming and sample intensive. Capillary electrophoresis (CE) is often adapted as an orthogonal alternative for biotherapeutic characterization because of its speed and sensitivity. However, the utility of CE is generally limited by poor compatibility with MS. ZipChip/MS overcomes these incompatibility issues enabling rapid in-depth characterization of biotherapeutics.
In the first part of the webinar, Dr. Kulkarni will provide an overview of the ZipChip/MS technology and explain the fundamental concepts behind how ZipChip/MS works. In the second part of the webinar, Dr. Smith will present results obtained by applying the ZipChip/MS to the characterization of Adeno-Associated Virus (AAV) capsid proteins. AAVs are viral vectors increasingly investigated for their clinical potential as delivery systems of gene therapy targets. Characterization of intact AAV capsids, their viral proteins (VPs), and their post-translational modifications (PTMs) is critical to monitoring and ensuring product quality and efficacy.
Dr. Smith will demonstrate how microchip CE-MS can be applied to the characterization of AAVs, specifically VP characterization and peptide mapping. Performed across a spectrum of AAV serotypes, we discuss how this platform quickly provides crucial information related to the characteristics of AAVs including the presence of VP proteoforms and PTMs.
[post_title] => In-Depth Adeno-Associated Virus Characterization with Microchip Capillary Electrophoresis-Mass Spectrometry [post_excerpt] => This webinar consists of two parts: Dr. Adi Kulkarni from 908 Devices begins by providing a brief introduction to the ZipChip CE-MS technology, highlighting its features and benefits. In part two, Dr. Josh Smith from NIBRT, demonstrates how microchip CE-MS can be applied to the characterization of AAVs, specifically VP characterization and peptide mapping. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-in-depth-adeno-associated-virus-characterization [to_ping] => [pinged] => [post_modified] => 2024-05-24 00:08:35 [post_modified_gmt] => 2024-05-24 04:08:35 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=23016 [menu_order] => 185 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [1] => WP_Post Object ( [ID] => 8043 [post_author] => 29 [post_date] => 2023-05-01 14:02:59 [post_date_gmt] => 2023-05-01 18:02:59 [post_content] =>About this Webinar
Peptide mapping is considered the gold standard for the analysis of biopharmaceuticals. Classical analysis is performed through long workflows involving extensive sample handling and long liquid chromatography (LC-MS) analysis times.
The distinct advantages of short analysis times and unique separation mechanisms makes Capillary Electrophoresis (CE-MS) an attractive alternative for the peptide mapping analysis. Nevertheless, most existing sample preparation protocols are either incompatible with CE-MS analysis or require additional steps for salt removal that may increase sample handling.
Key Takeaways
- An overview of the ZipChip Microchip CE-MS technology and its typical application space
- How to optimize CE-MS compatible sample preparation using a total workflow length of 2 hours
- Data analysis will provide full sequence coverage and accurate quantitation of product quality attributes
- Wide applicability of the workflow to different IgG subclasses will be demonstrated
- A panel discussion (Q&A) at the end
- Microchip CE-MS technology, its capabilities, and typical application space
- How CE-MS technology can enhance peptide mapping throughput
- Coupling peptide mapping sample preparation of monoclonal antibodies to CE-MS analysis
- CE-MS analysis provides robust determination of quality attributes in less than 15 minutes
Meet the Speakers
Sara Carillo
Dr. Sara Carillo completed her Ph.D. in Chemical Sciences in 2013 at the University of Naples “Federico II”. Under the guidance of Prof. Corsaro, she focused on the structural characterisation of polysaccharides and glyco-conjugates from Gram-negative bacteria via NMR and mass spectrometry techniques, focusing on the immunological properties and potentials of extremophiles endotoxins. After a period at the University College of Dublin, in 2015 she joined Dr. Jonathan Bones’s research group in NIBRT working on understanding of the effects of extractables and leachables from single-use bioreactors on CHO cells N-glycome and produced monoclonal antibodies. She is now working in NIBRT as Bioanalytical Research Lead and is mainly interested in the development of new analytical approaches to ensure a deeper and easier understanding of biomanufacturing processes and biopharmaceuticals’ structural complexity.
Adi Kulkarni
Adi Kulkarni is a Principal Scientist. Adi has extensive experience in applying CE-MS in biopharma characterization workflows using different mass spec platforms. He has developed novel applications based on the ZipChip technology and managing strategic collaborations and partnerships. Prior to working at 908 Devices, Adi was an applications scientist at Bruker Daltonics where he worked on Bruker’s Q-TOF line of products. He received his Ph.D. at the University of Massachusetts in 2011 where he developed novel methodologies for the synthesis of nitrogen-containing heterocycles. After his Ph.D., Adi was a postdoctoral fellow at University of Southern California’s Loker Hydrocarbon Research Institute where he discovered new reagents for applications in organofluorine chemistry. He has authored 18 peer-reviewed articles.
Jonathan Bones
Jonathan Bones is the principal investigator of the Characterisation and Comparability Laboratory at NIBRT. An analytical chemist by training, Jonathan’s research group specializes in the development and application of analytical solutions for problems associated with the manufacture and characterization of biopharmaceuticals. This includes recombinant proteins, monoclonal antibodies, and advanced therapies like AAV based gene therapy, cell therapy, and mRNA using their core excellence in analytical separations coupled to mass spectrometry.
Erin Redman
Erin Redman is a Principal Scientist in the Research and Development division of 908 Devices. She joined the company in 2016 and has worked to develop protein characterization assays using the ZipChip technology and further the innovation microfluidic CE separations coupled to mass spectrometry. Prior to starting her career at 908 Devices, she received her Ph.D. under the direction of Professor J. Michael Ramsey at the University of North Carolina at Chapel Hill. There she developed microfluidic technology for interfacing high resolution separations with electrospray mass spectrometry for the characterization of intact biotherapeutic proteins and proteins from biofluids.
[post_title] => Direct Microchip CE/MS Peptide Mapping Workflow for the Analysis of mAb CQAs [post_excerpt] => Microchip CE-MS technology, its capabilities, and typical application space. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-webinar-ls-zc-wb-0017 [to_ping] => [pinged] => [post_modified] => 2024-08-01 13:52:43 [post_modified_gmt] => 2024-08-01 17:52:43 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=8043 [menu_order] => 188 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [2] => WP_Post Object ( [ID] => 5044 [post_author] => 38 [post_date] => 2021-12-09 15:13:01 [post_date_gmt] => 2021-12-09 20:13:01 [post_content] =>[post_title] => Simplifying Oligonucleotide Characterization with Microchip CE-MS [post_excerpt] => In this on-demand webinar, we present a microfluidic capillary zone electrophoresis-based separation coupled with HRAM mass-spectrometry for rapid characterization of oligos in positive ionisation mode. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => simplifying-oligonucleotide-characterization-with-microchip-ce-ms [to_ping] => [pinged] => [post_modified] => 2024-10-02 06:15:56 [post_modified_gmt] => 2024-10-02 10:15:56 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=5044 [menu_order] => 284 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [3] => WP_Post Object ( [ID] => 1167 [post_author] => 38 [post_date] => 2020-12-17 21:19:38 [post_date_gmt] => 2020-12-18 02:19:38 [post_content] =>[post_title] => Fast Therapeutic Protein Characterization [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-webinar-tmo-fast-therapeutic-protein-characterization [to_ping] => [pinged] => [post_modified] => 2024-10-01 11:11:12 [post_modified_gmt] => 2024-10-01 15:11:12 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/resource/zipchip_webinar_tmo_fast-therapeutic-protein-characterization/ [menu_order] => 424 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) ) [post_count] => 4 [current_post] => -1 [before_loop] => 1 [in_the_loop] => [post] => WP_Post Object ( [ID] => 23016 [post_author] => 29 [post_date] => 2023-12-20 10:11:09 [post_date_gmt] => 2023-12-20 15:11:09 [post_content] =>About this Webinar
This webinar consists of two parts: Dr. Adi Kulkarni begins by providing a brief introduction to the ZipChip CE-MS technology, highlighting its features and benefits, followed by Dr. Josh Smith from NIBRT, with a deep dive of the ZipChip CE-MS technology applied to the characterization of AAVs.
Commonly utilized liquid chromatography-mass spectrometry (LC-MS) characterization strategies are time consuming and sample intensive. Capillary electrophoresis (CE) is often adapted as an orthogonal alternative for biotherapeutic characterization because of its speed and sensitivity. However, the utility of CE is generally limited by poor compatibility with MS. ZipChip/MS overcomes these incompatibility issues enabling rapid in-depth characterization of biotherapeutics.
In the first part of the webinar, Dr. Kulkarni will provide an overview of the ZipChip/MS technology and explain the fundamental concepts behind how ZipChip/MS works. In the second part of the webinar, Dr. Smith will present results obtained by applying the ZipChip/MS to the characterization of Adeno-Associated Virus (AAV) capsid proteins. AAVs are viral vectors increasingly investigated for their clinical potential as delivery systems of gene therapy targets. Characterization of intact AAV capsids, their viral proteins (VPs), and their post-translational modifications (PTMs) is critical to monitoring and ensuring product quality and efficacy.
Dr. Smith will demonstrate how microchip CE-MS can be applied to the characterization of AAVs, specifically VP characterization and peptide mapping. Performed across a spectrum of AAV serotypes, we discuss how this platform quickly provides crucial information related to the characteristics of AAVs including the presence of VP proteoforms and PTMs.
[post_title] => In-Depth Adeno-Associated Virus Characterization with Microchip Capillary Electrophoresis-Mass Spectrometry [post_excerpt] => This webinar consists of two parts: Dr. Adi Kulkarni from 908 Devices begins by providing a brief introduction to the ZipChip CE-MS technology, highlighting its features and benefits. In part two, Dr. Josh Smith from NIBRT, demonstrates how microchip CE-MS can be applied to the characterization of AAVs, specifically VP characterization and peptide mapping. [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => zipchip-in-depth-adeno-associated-virus-characterization [to_ping] => [pinged] => [post_modified] => 2024-05-24 00:08:35 [post_modified_gmt] => 2024-05-24 04:08:35 [post_content_filtered] => [post_parent] => 0 [guid] => https://908devices.com/?post_type=resource&p=23016 [menu_order] => 185 [post_type] => resource [post_mime_type] => [comment_count] => 0 [filter] => raw ) [comment_count] => 0 [current_comment] => -1 [found_posts] => 4 [max_num_pages] => 0 [max_num_comment_pages] => 0 [is_single] => [is_preview] => [is_page] => [is_archive] => 1 [is_date] => [is_year] => [is_month] => [is_day] => [is_time] => [is_author] => [is_category] => [is_tag] => [is_tax] => 1 [is_search] => [is_feed] => [is_comment_feed] => [is_trackback] => [is_home] => [is_privacy_policy] => [is_404] => [is_embed] => [is_paged] => [is_admin] => [is_attachment] => [is_singular] => [is_robots] => [is_favicon] => [is_posts_page] => [is_post_type_archive] => 1 [query_vars_hash:WP_Query:private] => 6a3a11a59666251ed44c74bb625f0256 [query_vars_changed:WP_Query:private] => 1 [thumbnails_cached] => [allow_query_attachment_by_filename:protected] => [stopwords:WP_Query:private] => [compat_fields:WP_Query:private] => Array ( [0] => query_vars_hash [1] => query_vars_changed ) [compat_methods:WP_Query:private] => Array ( [0] => init_query_flags [1] => parse_tax_query ) )
Webinars On Demand
In-Depth Adeno-Associated Virus Characterization with Microchip Capillary Electrophoresis-Mass Spectrometry
This webinar consists of two parts: Dr. Adi Kulkarni from 908 Devices begins by providing a brief introduction to the ZipChip CE-MS technology, highlighting its features and benefits. In part two, Dr. Josh Smith from NIBRT, demonstrates how microchip CE-MS can be applied to the characterization of AAVs, specifically VP characterization and peptide mapping.
Direct Microchip CE/MS Peptide Mapping Workflow for the Analysis of mAb CQAs
Microchip CE-MS technology, its capabilities, and typical application space.
Simplifying Oligonucleotide Characterization with Microchip CE-MS
In this on-demand webinar, we present a microfluidic capillary zone electrophoresis-based separation coupled with HRAM mass-spectrometry for rapid characterization of oligos in positive ionisation mode.
Fast Therapeutic Protein Characterization
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=> [post_title] => Microchip CE-MS: A Powerful Tool for Biotherapeutic Characterization [post_excerpt] => To help explain the power of this game-changing technology, we partnered with LCGC on a new ebook that covers the topic in-depth. 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