Thank you to all that attended our
Breakfast Seminar

Monday, June 5
7:00-8:00AM
Room 342AB-George R. Brown Convention Center

Protein Characterization of Biologics using Microchip CE-MS: From mAbs to AAVs

Protein characterization is crucial to ensure the quality of biotherapeutics during the drug discovery and development process. A wide variety of analytical techniques are needed to achieve a thorough understanding of the critical product quality attributes impacting drug safety and efficacy. Capillary zone electrophoresis (CZE) is a separation technique that can produce highly efficient separations of a wide variety of biological molecules. The ZipChip (908 Devices) is a microfluidic device that seamlessly couples rapid & high resolution CZE separations to high resolution accurate mass (HRAM) mass spectrometry, making it a powerful analytical platform that can be used for a wide range of applications, ranging from biopharmaceutical drug product characterization, clinical diagnostics, cell and gene therapies, proteomics, and metabolomics.

Join us to hear Dr. Jonathan Bones and Dr. Josh Smith from the National Institute of Bioprocessing Research and Training (NIBRT) share application highlights of using microchip CE-MS for protein characterization. In the first part of this presentation, Dr. Bones will share data showing his lab’s use of ZipChip for multi-characterization of proteins in applications including Charge Variant Analysis, Peptide Mapping, and Released Glycan Analysis of therapeutic proteins. He will also discuss the key advantages of ZipChip such as versatility, high sensitivity, short analysis times and lack of method development as applicable in these workflows.

In the second part of this presentation, Dr. Smith will highlight the use of ZipChip for the analysis of the Adeno-Associated Viruses (AAVs). He will discuss the method development work, which went into successfully using the ZipChip/MS for rapid analysis of various serotypes of the AAV viral capsid proteins (VPs). He will also share the tips and tricks to rapidly characterize AAV capsid proteins using intact mass analysis and obtaining near 100% sequence coverage for VPs using the peptide mapping workflow.

Meet our speakers:

Jonathan is the principal investigator of the Characterization and Comparability Laboratory at NIBRT. An analytical chemist by training, Jonathan’s research group specializes in the development and application of analytical solutions for problems associated with the manufacture and characterization of biopharmaceuticals including recombinant proteins, monoclonal antibodies and advanced therapies including AAV based gene therapy, cell therapy and mRNA using their core excellence in analytical separations coupled to mass spectrometry.

Presentation PDF

Dr. Jonathan Bones
NIBRT

Josh is a Senior Research Scientist in the Characterization and Comparability Lab at NIBRT in Dublin, Ireland. He initially joined NIBRT to undertake his Ph.D. in analytical biochemistry in conjunction with Trinity College Dublin. His PhD studies focused on developing liquid chromatography-mass spectrometry omics platforms applied to in-depth serum characterization for early-stage biomarker discovery. Josh subsequently took a role evaluating the impact of production media quality on monoclonal antibody critical quality attributes, before transitioning to his current position where he develops and applies mass spectrometry workflows for the characterization of viral gene therapy products and their host cell protein contaminants.

Presentation PDF

Dr Josh Smith
NIBRT