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[post_title] => RPMI 1640 Medium Analysis with the REBEL: Accuracy and Reproducibility Across Multiple Devices
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[post_title] => Screening for Component Diversity in CHO Cell Culture Media
[post_excerpt] => At-line amino acid analysis can speeds up cell culture media and feeding strategy optimization for bioprocess development.
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[post_title] => High variations of analyte levels in Terrific broth (TB) media
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[post_title] => Application Snapshot Booklet Cell and Gene Therapy
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[post_title] => How comparable are different vendors' LB media?
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[post_title] => Investigating the high nutrient variations in Super Optimal Broth
[post_excerpt] => Cultures’ growth rates and productivities may differ when different vendor’s media are used in otherwise identical experiments.
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[post_title] => Similarities between different vendors’ 2X YT liquid media
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[post_title] => At-line spent media analysis is now a reality
[post_excerpt] => To elevate process development decisions to real-time, researchers require new analytical platforms that simplify analysis and accelerate delivery of actionable intelligence.
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[post_title] => Differences in fresh commercially available hybridoma media
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[post_title] => Select the best CHO media from the start
[post_excerpt] => Performing your own quick assays of the media you receive ensures consistency in the cell media fed to your cells.
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[post_title] => Screening of chemically-defined media for suspension cultures of HEK293 cells
[post_excerpt] => Across four vendors, there was a high variation in the fresh media composition.
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[post_title] => Analyze media without removing your mAb on the Rebel
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[post_title] => Identify variations in dry and liquid CHO media
[post_excerpt] => Running a quick fresh media assay prior to feeding the culture ensures consistency of the media components when scaling up or during the transition between liquid-to-dry media blends.
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[post_title] => Serum supplemented media analysis is routine with the Rebel
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Application Notes & Snapshots
RPMI 1640 Medium Analysis with the REBEL: Accuracy and Reproducibility Across Multiple Devices
This extensive fresh media analysis experiment provides an example of the accuracy and reproducibility of the REBEL device obtained with RPMI media.
REBEL
Application Notes & Snapshots
Download
Screening for Component Diversity in CHO Cell Culture Media
At-line amino acid analysis can speeds up cell culture media and feeding strategy optimization for bioprocess development.
REBEL
Application Notes & Snapshots
Download
High variations of analyte levels in Terrific broth (TB) media
REBEL
Application Notes & Snapshots
Download
Application Snapshot Booklet Cell and Gene Therapy
REBEL
Application Notes & Snapshots
Download
How comparable are different vendors’ LB media?
REBEL
Application Notes & Snapshots
Download
Investigating the high nutrient variations in Super Optimal Broth
Cultures’ growth rates and productivities may differ when different vendor’s media are used in otherwise identical experiments.
REBEL
Application Notes & Snapshots
Download
Probing formulation differences in Super Optimal Broth
REBEL
Application Notes & Snapshots
Download
Similarities between different vendors’ 2X YT liquid media
REBEL
Application Notes & Snapshots
Download
At-line spent media analysis is now a reality
To elevate process development decisions to real-time, researchers require new analytical platforms that simplify analysis and accelerate delivery of actionable intelligence.
REBEL
Application Notes & Snapshots
Download
Differences in fresh commercially available hybridoma media
REBEL
Application Notes & Snapshots
Download
Select the best CHO media from the start
Performing your own quick assays of the media you receive ensures consistency in the cell media fed to your cells.
REBEL
Application Notes & Snapshots
Download
Screening of chemically-defined media for suspension cultures of HEK293 cells
Across four vendors, there was a high variation in the fresh media composition.
REBEL
Application Notes & Snapshots
Download
Analyze media without removing your mAb on the Rebel
REBEL
Application Notes & Snapshots
Download
Identify variations in dry and liquid CHO media
Running a quick fresh media assay prior to feeding the culture ensures consistency of the media components when scaling up or during the transition between liquid-to-dry media blends.
REBEL
Application Notes & Snapshots
Download
Serum supplemented media analysis is routine with the Rebel
REBEL
Application Notes & Snapshots
Download
Supplementing IMDM media with fetal bovine serum
REBEL
Application Notes & Snapshots
Download
Supplementing RPMI media with fetal bovine serum
REBEL
Application Notes & Snapshots
Download
Conventional and chemically-defined T cell medias
REBEL
Application Notes & Snapshots
Download
Amino acid contents of Sf9 and Sf21 insect cell media
Knowledge of the insect cell metabolism coupled with an understanding of the amino acid content of the cell media allows for a more informed process development analytics strategy.
REBEL
Application Notes & Snapshots
Download
Comparing mesenchymal stem cell media with supplements on the Rebel
To ensure consistency when culturing MSCs, researchers should understand what is being added to their media.
REBEL
Application Notes & Snapshots
Download
Don’t sweat your cell media additives
REBEL
Application Notes & Snapshots
Download
Handle non-ionic surfactants in cell media samples with ease
REBEL
Application Notes & Snapshots
Download
Basal cell media with 10% fetal bovine serum
REBEL
Application Notes & Snapshots
Download
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Brochures & Flyers
REBEL Certified User Training
Get the best user experience with the REBEL at-line spent media analyzer. Our training course is for new and experienced users who want to refresh their REBEL device
operation skills.
REBEL
Brochures & Flyers
Download
REBEL Spent Media Analysis Kits
The REBEL Spent Media Analysis (SMA) kit is designed to give you all the consumable components needed to run your samples quickly and move your projects forward faster.
REBEL
Brochures & Flyers
Download
REBEL Brochure
The REBEL is a novel, dedicated cell culture media analyzer designed for rapid, easy, and efficient amino acid and nutrient analysis.
REBEL
Brochures & Flyers
Download
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[post_title] => Amino Acid Analysis Indicates Metabolic Differences in Multi-Cytokine Backpack - Manufactured CAR T-Cells
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[post_title] => Optimization of Cell Culture Media and Culture Conditions for HEK293 for Improved Cell Growth and Reduced Cell Clumping for the Effective Production of Viral Vectors
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[post_title] => Optimization of Scalable rAAV Production for Gene Therapy
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[post_title] => Empowering Process Optimization for a mAb-Expressing CHO Cell Line
[post_excerpt] => Coupling miniature mass spectrometry (MS) with microchip capillary electrophoresis (CE) fulfills an unmet need: simple, rapid at-line nutrient quantitation.
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Posters
Know Your Process: On-line and At-line Analytics for Upstream Bioprocess Key Nutrients
Bioprocess development requires analytics to monitor critical process parameters. This poster discusses two discuss two key technologies to characterize, optimize and control bioprocess. This was poster B03 at BPS Europe 2024.
MAVEN
REBEL
Posters
Download
Amino Acid Quantitation in Over 50 Mammalian Cell Culture Media With an Integrated CE-MS Analyzer
Characterizing cell culture nutrients such as amino acids in growth media is critical for maximizing cell growth and improving key quality attributes of the cells. This poster was presented at ASMS 2023 as poster THP625.
REBEL
Posters
Download
Amino Acid Quantitation of Cell Culture Media Matrices via an Integrated CE-MS Analyzer
Optimization of cell culture media and process conditions are critical for achieving optimal cell growth, productivity, and product quality. This poster was presented at ASMS 2023 as poster TP 398.
REBEL
Posters
Download
Optimization of Scalable rAAV Production for Gene Therapy – Leveraging At-Line Amino Acid Measurements for Bioprocess Modeling-Driven Approaches
Spent media analysis from an at-line analyzer, the REBEL, was used to further understanding of AAV production process and identify correlation between key nutrient consumptions and capsid titers in fed-batch and continuous processes. View our poster from the 2024 Advanced Manufacturing Cell and Gene Therapies Conference. This was poster 43.
REBEL
Posters
Download
Improving Cell and Gene Therapy Manufacturing Processes by Automated On-line and In-line Bioprocess Analytical Technologies
Recent advances in cell and gene therapy bioprocess analytical technology regimes, approaches to de novo analytical modeling/calibration of these systems, and how their informing power can be leveraged with the increasing sophistication of bioprocess metabolic models for predictive bioprocess optimization. View our poster from the 2024 Advanced Manufacturing Cell and Gene Therapies Conference. This was poster 29.
MAVEN
MAVERICK
REBEL
ZipChip
Posters
Download
Amino Acid Analysis Indicates Metabolic Differences in Multi-Cytokine Backpack – Manufactured CAR T-Cells
Novel reagents that aid in the consistent manufacturing of
CAR T-cells with well-defined phenotypes are needed to improve treatment outcomes in patients. View our poster from the 2024 Advanced Manufacturing Cell and Gene Therapies Conference. This was poster 17.
REBEL
Posters
Download
Optimization of Cell Culture Media and Culture Conditions for HEK293 for Improved Cell Growth and Reduced Cell Clumping for the Effective Production of Viral Vectors
This study demonstrates the power of at-line cell culture analyzers such as the REBEL to gain process understanding quickly to adjust media components for improved cell culture performance. View our poster from the 2024 Advanced Manufacturing Cell and Gene Therapies Conference. This was poster 18.
REBEL
Posters
Download
Optimization of Scalable rAAV Production for Gene Therapy
This poster showcases spent media analysis leveraging REBEL, which requires only 10 μL per sample and 10 minutes analysis time.
REBEL
Posters
Download
Empowering Process Optimization for a mAb-Expressing CHO Cell Line
Coupling miniature mass spectrometry (MS) with microchip capillary electrophoresis (CE) fulfills an unmet need: simple, rapid at-line nutrient quantitation.
REBEL
Posters
Download
Using genomic and spent media analysis to characterize stressed CHO cultures
REBEL
Posters
Download
A Rapid Microfluidic Method for Molecular Weight Determination and Spent Media Analysis of an IgG1 Intact Protein in Growth Media
REBEL
ZipChip
Posters
Download
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Published Articles
In-Process Analytics are Key to Successful Cell Therapy Process Optimization and Manufacturing
Considerable clinical activity and the approval of several autologous, patient-specific therapies has increased industry demand and has also highlighted bottlenecks in manufacturing workflows.
REBEL
Published Articles
Download
Harness the Power of At-Line Spent Media Analysis (BioPharm International)
REBEL
Published Articles
Download
Spent Media Analysis of CHO Grown in an Ammonia-Stressed Parallel Microbioreactor Platform
REBEL
Published Articles
Download
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Testimonials
Use of Mass Spectrometry to Assist in Biopharmaceutical Process Development
Hear from Chris McCredy from Sartorius. He discusses how using at-line analytics are valuable from process modeling and biosimulation.
REBEL
Testimonials
Download
Addressing Upstream Cell Culture Challenges as a Community
Hear from Mike Betenbaugh and see how the Advanced Mammalian Biomanufacturing Innovation
Center (AMBIC) is working with bioprocess modeling
REBEL
Testimonials
Download
Hear from Our Users: The Need for Speed in the Lab
Listen to ZipChip and REBEL users from The Dana Farber Cancer Institute, Sartorius, and Amgen discuss the impact of faster workflows in the lab.
REBEL
ZipChip
Testimonials
Download
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Videos
Ultimate Bioprocess Monitoring and Control
Quickly get data at the point of need with our REBEL, MAVEN, and MAVERICK devices. Take control of your bioprocess and get to market faster.
MAVEN
MAVERICK
REBEL
Videos
Watch Video
REBEL Product Video
REBEL
Videos
Watch Video
Poster Chat: Spent Media Analysis of CHO
REBEL
Videos
Watch Video
Rapid at-line nutrient profiling from an ammonia stressed CHO cell line
REBEL
Videos
Watch Video
Booth Chat
REBEL
Videos
Watch Video
REBEL Demo
REBEL
Videos
Watch Video
Rapid analysis of amino acids, water soluble vitamins, biogenic amines and dipeptides in cell growth media
REBEL
Videos
Watch Video
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About this Webinar
Despite the remarkable clinical efficacy of chimeric antigen receptor (CAR) T cells in hematological malignancies, only a subset of patients achieves a durable complete response (dCR). DCR has been correlated with CAR T cell products enriched with T cells of memory phenotypes. Therefore, reagents that consistently promote memory phenotypes during the manufacturing of CAR T cell products have the potential to significantly improve clinical outcomes. Researchers at Emory University, led by Dr. Sarwish Rafiq, have developed a novel modular multi-cytokine backpack (MCB) platform that combines the signals necessary for activation, co-stimulation, and cytokine support into a single “all-in-one” reagent for CAR T cell manufacturing.
Key Takeaways
- About a novel multi-cytokine backpack MCB activation, co-stimulation, ad cytokine support for effective CAR T cell product manufacturing
- A proof-of-principle of the powerful utility of the MCB platform to identify reagents that can improve the effectiveness of cell therapy products through optimal manufacturing
- Indications of differences in the metabolism of the MCB-manufactured T cells through spent media amino acid analysis
[post_title] => Engineering Functionally Improved CAR T cells with a Multi-Cytokine Backpack Platform with Insights into Metabolism
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About this Webinar
A broad range of separation techniques are now routinely combined with mass spectrometry for in-depth biopharmaceutical characterization. Simultaneously, MS software tools have evolved to support the storage, processing, and reporting of the expanding MS data oceans. Here recent progress, challenges, and learnings from MS-based workflows of biopharmaceutical charge variants will be presented and illustrated through case-studies.
Key Takeaways
- The crucial role that CE-MS plays in a biopharma development lab for the comprehensive characterization of biopharmaceuticals.
- How CE-MS can be used to improve the efficiency of characterization workflows by eliminating method development and increasing the throughput while gaining deeper insights through in-depth analysis of biopharmaceuticals.
[post_title] => Accelerate Upstream Process Development With Data-driven Feeding Strategy Optimization
[post_excerpt] => At-line and on-line technologies that inform bioprocess parameters and cell-feeding strategies are needed to develop processes that deliver high-quality products while reducing manufacturing costs. In this webinar, Chris Schultz, PhD from CPI provides insights on using at-line spent media analysis and on-line glucose and lactate monitoring for the optimization of feeding strategy.
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[post_title] => Harness the Power of At-line Spend Media Analysis using the REBEL to Optimize a CHO mAb Fed-batch Process
[post_excerpt] => This webcast shares a data-driven and accelerated cell culture media screening as well as the selection in the Ambr15 microbioreactor system.
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Controlling Essential Amino Acid Levels in CHO Cell Cultures Using A Genome-Scale Nutrient Minimization Forecast Algorithm
Our own Milla Neffling moderates a discussion with three authors of a recent peer-reviewed article in Biotechnology & Bioengineering. The 18-minute discussion ranges from the importance of process modeling to the critical role of new tools like REBEL.
Key Discussion Points:
- How media optimization strategies evolved to help the biopharma industry
- The benefit of genome-scale models
- The importance of input data quality
- The relevance of amino acids and their importance in genome-scale models
Speakers:
Michael Betenbaugh, Ph.D
Professor,
Johns Hopkins University
Yiqun Chen, Ph.D
Graduate Student,
Johns Hopkins University
Ji Young Anderson
Sr. Scientist,
908 Devices
Milla Neffling
Bioprocessing Segment Manager
908 Devices
About this Webinar
Despite the remarkable clinical efficacy of chimeric antigen receptor (CAR) T cells in hematological malignancies, only a subset of patients achieves a durable complete response (dCR). DCR has been correlated with CAR T cell products enriched with T cells of memory phenotypes. Therefore, reagents that consistently promote memory phenotypes during the manufacturing of CAR T cell products have the potential to significantly improve clinical outcomes. Researchers at Emory University, led by Dr. Sarwish Rafiq, have developed a novel modular multi-cytokine backpack (MCB) platform that combines the signals necessary for activation, co-stimulation, and cytokine support into a single “all-in-one” reagent for CAR T cell manufacturing.
Key Takeaways
- About a novel multi-cytokine backpack MCB activation, co-stimulation, ad cytokine support for effective CAR T cell product manufacturing
- A proof-of-principle of the powerful utility of the MCB platform to identify reagents that can improve the effectiveness of cell therapy products through optimal manufacturing
- Indications of differences in the metabolism of the MCB-manufactured T cells through spent media amino acid analysis
Webinars On Demand
Engineering Functionally Improved CAR T cells with a Multi-Cytokine Backpack Platform with Insights into Metabolism
REBEL
Webinars On Demand
Watch Webinar
Accelerate Upstream Process Development With Data-driven Feeding Strategy Optimization
At-line and on-line technologies that inform bioprocess parameters and cell-feeding strategies are needed to develop processes that deliver high-quality products while reducing manufacturing costs. In this webinar, Chris Schultz, PhD from CPI provides insights on using at-line spent media analysis and on-line glucose and lactate monitoring for the optimization of feeding strategy.
MAVEN
REBEL
Webinars On Demand
Watch Webinar
Harness the Power of At-line Spend Media Analysis using the REBEL to Optimize a CHO mAb Fed-batch Process
This webcast shares a data-driven and accelerated cell culture media screening as well as the selection in the Ambr15 microbioreactor system.
REBEL
Webinars On Demand
Watch Webinar
Power Up Your Process Control with Rapid At-Line MS-based Metabolite Monitoring
Graziella Piras spoke at BPI 2022 about how amino acids can present a goldilocks problem for bioprocessing: too little and they starve; too much and they favor growth over protein production.
REBEL
Webinars On Demand
Watch Webinar
Enabling Faster Feed Strategy Optimization with Rapid Spent Media Analysis and Data Visualization Tools
Learn how to take charge of your bioprocess development via at-line amino acid analysis empowered by a dedicated bioprocess software tool.
REBEL
Webinars On Demand
Watch Webinar
Multi-Component At-Line Cell Culture Media Analysis for Accelerated Process Development of a mAb-Expressing Cell Line
In this talk, Graziella Piras discusses a data-driven and accelerated media screening, selection, and optimization experiment leveraging REBEL.
REBEL
Webinars On Demand
Watch Webinar
Biopharma 4.0 and Cell Media Optimization & At-line Methods for Cell Media Analysis
Graziella Piras, bioprocess segment director for 908 Devices, spoke at BPI Europe 2022 on the benefits of at-line nutrient monitoring.
REBEL
Webinars On Demand
Watch Webinar
The Critical Role of Cell Culture Media Analysis in Cell and Gene Therapies Development
Does cell culture analysis matter for CGT development? This short webinar explores what is already known, reviews at-line methods for cell culture media analysis, and shares recent data from two case studies.
REBEL
Webinars On Demand
Watch Webinar
Biopharma 4.0: Finding Opportunities to Leverage New At-Line Data
Listen how REBEL users describe how they use better data acquisition, management platforms, and process modeling to implement process intensification.
REBEL
Webinars On Demand
Watch Webinar
Process Modeling with Researchers from Johns Hopkins University
Listen to a dynamic discussion on the highlights of a new peer-reviewed article exploring model-based approached to cell culture optimization
REBEL
Webinars On Demand
Watch Webinar
![[Webinar] Bioprocess 101: Cell Culture Media Analysis](https://908devices.com/wp-content/uploads/2022/01/9080151_Bioprocess101-Cell-Culture-Analysis_R1-300x300.jpg)
Ask the Expert: Bioprocess 101 – Cell Culture Media Analysis
REBEL
Webinars On Demand
Watch Webinar
Conference Talk: 2021 Industrializing Cell-Based Meats & Seafood Summit
REBEL
Webinars On Demand
Watch Webinar
The Importance of Cell Media Analysis in Upstream Bioprocess Development
Watch this discussion with experts from industry and academia as they discuss bioprocessing trends relating to cell culture media analysis.
REBEL
Webinars On Demand
Watch Webinar
Mass Spectrometry-Based Process Analytical Technologies for Cell Therapies
REBEL
Webinars On Demand
Watch Webinar
Unlocking Rapid Cell Culture Media Analysis for Cell and Gene Therapy Process Development
REBEL
Webinars On Demand
Watch Webinar
Be a REBEL with Your Cell Media Analytics
REBEL
Webinars On Demand
Watch Webinar
Redefine Bioprocess Analytics
REBEL
ZipChip
Webinars On Demand
Watch Webinar
Rapid At-line Amino Acid and Nutrient Profiling from Bioprocess Media with the REBEL
REBEL
Webinars On Demand
Watch Webinar
How can we Accelerate PD Analytics?
REBEL
Webinars On Demand
Watch Webinar
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