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Application Notes & Snapshots
Microchip CE/MS Peptide Mapping Workflow with Trypsin Digestion for the Analysis of Critical Quality Attributes of Monoclonal Antibodies
Peptide mapping is considered the gold standard for the analysis of biopharmaceuticals, including monoclonal antibodies.
Poster Note: Bottom-up analysis of progressively larger RNAs by CZE-MS and CZE-MS/MS
This project explores the merits of CZE as a potential alternative to liquid chromatography, as the front-end separation of choice for analyzing progressively larger RNAs with a bottom-up approach.
Poster Note: Multi-Level Expeditious Characterization of AAV Capsid Proteins using ZipChip
This poster note presents a novel approach to complete characterization of capsid viral proteins using microchip CE-MS for the characterization of AAVs.
Poster Note: Rapid Characterization of Oligos Using Microchip CE-MS without Ion-Pairing Reagents
Learn how ZipChip’s ready-to-use oligos BGE solution is free from ion-pairing reagents and makes switching between applications simple and easy.
Application Report: Glucose Control
The online analyser TRACE C2 Control allows a rapid and precise determination of glucose and lactate concentrations inside the bioreactor.
Application Report: Ethanol Dialysis
The online analyzer TRACE C2 Control allows a rapid and precise determination of the Ethanol concentration in the fermenter.
Application Report: Methanol Dialysis
The online analyzer TRACE C2 Control allows a rapid and precise determination of the methanol concentration in the fermenter and provides the tools for a quick set-up of a feed control strategy
Application Report: Glucose & Lactate (in Small Bioreactors)
The online analyser TRACE C2 Control allows a rapid and precise determination of glucose and lactate concentrations inside small bioreactors.
Application Report: Glucose & Lactate
The online analyzer TRACE C2 Control allows a rapid and precise determination of glucose and lactate concentrations inside the bioreactor.
Quick screening of intact antibody and antibody drug conjugates with integrated microfluidic capillary electrophoresis and mass spectrometry
This application note shows a method for rapid screening of the heterogeneity of monoclonal antibodies and antibody-drug conjugates by an integrated microfluidic capillary electrophoresis (CE) and mass spectrometry (MS) workflow.
Rapid characterization of oligonucleotides using microfluidic CE-MS by ZipChip
This application note presents a CE-MS analysis of oligos by a ZipChip coupled to a QExactive HF mass spectrometer. This positive ESI mode method utilizes a MS-friendly background electrolyte (BGE), that eliminates the use of ion-paring agents such as hexafluoro isopropanol or alkyl amines.
Charge variant analysis with ZipChip and maXisII
A ZipChip capillary zone electrophoresis system coupled to Bruker maXis II UHR-QTOF offers an alternative selectivity to separate charge variants prior to MS analysis and affords a more complete intact mass characterization without adding complexity or high salt eluents.
Glucose and Lactate Raman-Based Monitoring with MAVERICK: Performance in Spent Media
MAVERICK provides a plug-and-play, in-line, Raman based PAT solution for monitoring and control of glucose, lactate, and biomass in up to 6 bioreactors. This Application Snapshot shows the results from experiments to demonstrate the selectivity of glucose and lactate measurements from MAVERICK in complex spent media samples from CHO and HEK293 cultures.
A ZipChip Based CZE-MS Analysis for Quick Assessment of Biotherapeutics
From comprehensive characterization to monitoring product degradation characteristics
Charge Variant Analysis Coupled to a SCIEX TripleTOF® 6600+ System Mass Spectrometer
High-Throughput Intact Mass Analysis of mAb Based Therapeutics
RPMI 1640 Medium Analysis with the REBEL: Accuracy and Reproducibility Across Multiple Devices
This extensive fresh media analysis experiment provides an example of the accuracy and reproducibility of the REBEL device obtained with RPMI media.
Screening for Component Diversity in CHO Cell Culture Media
At-line amino acid analysis can speeds up cell culture media and feeding strategy optimization for bioprocess development.
Application Snapshot Booklet Fermentation
Application Snapshot Booklet Cell and Gene Therapy
Application Snapshot Booklet Cell Culture
Application Snapshot Booklet Biologics
Application Snapshot Booklet
Download our full collection of application snapshots, with focuses on biologics, CGT, and fermentation.
How comparable are different vendors’ LB media?
High variations of analyte levels in Terrific broth (TB) media
Investigating the high nutrient variations in Super Optimal Broth
Cultures’ growth rates and productivities may differ when different vendor’s media are used in otherwise identical experiments.
Probing formulation differences in Super Optimal Broth
Analysis of Reduced mAbs and ADCs
Similarities between different vendors’ 2X YT liquid media
Rapid Characterization of Intact mAb-based Biotherapeutics
Quick screening of intact antibody and ADC
Comparing standard microbial media from a single supplier
ZipChip for profiling and characterization of antibody charge heterogeneity
Sciex Flexible Solution for MAM with ZipChip
At-line spent media analysis is now a reality
To elevate process development decisions to real-time, researchers require new analytical platforms that simplify analysis and accelerate delivery of actionable intelligence.
Differences in fresh commercially available hybridoma media
Select the best CHO media from the start
Performing your own quick assays of the media you receive ensures consistency in the cell media fed to your cells.
Screening of chemically-defined media for suspension cultures of HEK293 cells
Across four vendors, there was a high variation in the fresh media composition.
Sciex Presents Charge Heterogeneity of Monoclonal Antibodies with ZipChip
Analyze media without removing your mAb on the Rebel
Identify variations in dry and liquid CHO media
Running a quick fresh media assay prior to feeding the culture ensures consistency of the media components when scaling up or during the transition between liquid-to-dry media blends.
Snapshot: Charge Variant Analysis
Charge Variant Analysis of Native Antibodies for In-depth Characterization of mAb-based Biotherapeutics
Serum supplemented media analysis is routine with the Rebel
Bioreactor Monitoring via ZipChip
Tease out variations in dry cell culture media
Running a simple media assay prior to feeding your cells can ensure that the composition of the media is what is expected.
Snapshot: High-Throughput Intact Mass
Snapshot: High Throughput Charge Variant Analysis
Snapshot: Cell Culture Analysis
Supplementing RPMI media with fetal bovine serum
Supplementing IMDM media with fetal bovine serum
Conventional and chemically-defined T cell medias
Amino acid contents of Sf9 and Sf21 insect cell media
Knowledge of the insect cell metabolism coupled with an understanding of the amino acid content of the cell media allows for a more informed process development analytics strategy.
Comparing mesenchymal stem cell media with supplements on the Rebel
To ensure consistency when culturing MSCs, researchers should understand what is being added to their media.
Don’t sweat your cell media additives
Handle non-ionic surfactants in cell media samples with ease
Basal cell media with 10% fetal bovine serum
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