Application Notes & Snapshots

Poster Note: Bottom-up analysis of progressively larger RNAs by CZE-MS and CZE-MS/MS

This project explores the merits of CZE as a potential alternative to liquid chromatography, as the front-end separation of choice for analyzing progressively larger RNAs with a bottom-up approach.

Poster Note: Multi-Level Expeditious Characterization of AAV Capsid Proteins using ZipChip

This poster note presents a novel approach to complete characterization of capsid viral proteins using microchip CE-MS for the characterization of AAVs.

Poster Note: Rapid Characterization of Oligos Using Microchip CE-MS without Ion-Pairing Reagents

Learn how ZipChip’s ready-to-use oligos BGE solution is free from ion-pairing reagents and makes switching between applications simple and easy.

Quick screening of intact antibody and antibody drug conjugates with integrated microfluidic capillary electrophoresis and mass spectrometry

This application note shows a method for rapid screening of the heterogeneity of monoclonal antibodies and antibody-drug conjugates by an integrated microfluidic capillary electrophoresis (CE) and mass spectrometry (MS) workflow.

Rapid characterization of oligonucleotides using microfluidic CE-MS by ZipChip

This application note presents a CE-MS analysis of oligos by a ZipChip coupled to a QExactive HF mass spectrometer. This positive ESI mode method utilizes a MS-friendly background electrolyte (BGE), that eliminates the use of ion-paring agents such as hexafluoro isopropanol or alkyl amines.

Charge variant analysis with ZipChip and maXisII

A ZipChip capillary zone electrophoresis system coupled to Bruker maXis II UHR-QTOF offers an alternative selectivity to separate charge variants prior to MS analysis and affords a more complete intact mass characterization without adding complexity or high salt eluents.

A ZipChip Based CZE-MS Analysis for Quick Assessment of Biotherapeutics

From comprehensive characterization to monitoring product degradation characteristics

Charge Variant Analysis Coupled to a SCIEX TripleTOF® 6600+ System Mass Spectrometer

High-Throughput Intact Mass Analysis of mAb Based Therapeutics

Analysis of Reduced mAbs and ADCs

Rapid Characterization of Intact mAb-based Biotherapeutics

Quick screening of intact antibody and ADC

ZipChip for profiling and characterization of antibody charge heterogeneity

Sciex Flexible Solution for MAM with ZipChip

Sciex Presents Charge Heterogeneity of Monoclonal Antibodies with ZipChip

Snapshot: Charge Variant Analysis

Charge Variant Analysis of Native Antibodies for In-depth Characterization of mAb-based Biotherapeutics

Bioreactor Monitoring via ZipChip

Snapshot: High-Throughput Intact Mass

Snapshot: High Throughput Charge Variant Analysis

Snapshot: Cell Culture Analysis

Posters

Poster Note: Bottom-up analysis of progressively larger RNAs by CZE-MS and CZE-MS/MS

This project explores the merits of CZE as a potential alternative to liquid chromatography, as the front-end separation of choice for analyzing progressively larger RNAs with a bottom-up approach.

Poster Note: Multi-Level Expeditious Characterization of AAV Capsid Proteins using ZipChip

This poster note presents a novel approach to complete characterization of capsid viral proteins using microchip CE-MS for the characterization of AAVs.

Poster Note: Rapid Characterization of Oligos Using Microchip CE-MS without Ion-Pairing Reagents

Learn how ZipChip’s ready-to-use oligos BGE solution is free from ion-pairing reagents and makes switching between applications simple and easy.

ASMS2022 ThP362 Poster

Bottom-Up Analysis of Progressively Larger RNAs by CZE-MS and CZE-MS/MS

In this poster from ASMS 2022, researchers from University of Connecticut and Bruker showed that capillary zone electrophoresis performed on a chip proved to be an effective alternative to liquid chromatography as a front-end separation system to analyze both pure samples and complex digestion mixtures of nucleic acids.

ASMS2022 ThP360 Poster

Multi-level Expeditious Characterization of Adeno-associated Virus (AAV) Capsid Proteins Using ZipChip Microfluidic CE Coupled with HRMS

In this poster from ASMS 2022, scientists from Thermo Fisher Scientific and 908 Devices present a novel approach using microchip CE-MS for the characterization of AAVs.

ASMS2022 WP233 Poster

A Sensitive and High-Throughput Assay for Analyzing Peptide Exchanged MHCl by High-Resolution Native CZE-MS

In this poster from ASMS 2022, researchers from Genentech describe a sensitive and high-throughput assay using capillary-zone electrophoresis coupled to a high-resolution mass spectrometer for characterizing peptide-exchanged MHCI under native conditions.

ASMS2022 WP144 Poster

Combining μSPE ZipChip CE with PRM-LIVE on a timsTOF Pro for Warp-speed Selectivity Profiling of Deubiquitinase (DUB) Small Molecule Inhibitors

In this ASMS 2022 poster, researchers from Dana Farber Cancer Institute and Harvard Medical School developed a ZipChip-based method for facilitating high-throughput identification
of new small molecule inhibitors.

ASMS2022 WP044 Poster

Microchip CE-MS Analysis of Sialylated N-glycan Linkage Isomers

In this ASMS 2022 poster, Fudan University researchers show a ZipChip-based strategy for qualitative and quantitative analysis of sialylated N-glycan linkage isomers was developed.

ASMS2022 WP396 Poster

CE-MS of Oligonucleotides using a ZipChip

This ASMS 2022 poster from Boehringer Ingelheim explores the use of ZipChip coupled to a high resolution mass spectrometer as an orthogonal method to chromatography for oligonucleotide analysis.

ASMS2022 TP025 Poster

Glycosylation Profiling of mAB Subunits With CZE-MS

This ASMS 2022 poster from Bruker shows ZipChip coupled with high resolution mass spectrometry providing charge-based selectivity which can assist with glycoforms separation.

Rapid Characterization of Oligos and Related Impurities Using Microchip CE-MS Without Ion-pairing Reagents

This poster from ASMS 2022 describes a simple & fast method for oligonucleotide characterizations using a Microchip CE-MS platform.

Pushing the Boundaries of Speed and Sensitivity in Proteomics

To overcome sample loading limitations in CE-MS, the poster authors engineered a 1 mm SPE bed within the chip geometry, allowing 100-fold improvements in concentration sensitivity limit for CE-MS.

ASMS2022 MP347 Poster

A Broad Application of Migration Time Indexing and Metabolite Libraries to Evaluate Quantitative Coverage in Microchip CE-MS Metabolomics

In the field of metabolomics, microchip capillary electrophoresis (mCE) has a number of potential advantages over traditional liquid chromatography (LC), including higher throughput due to decreased separation times, increased separation efficiency, and lower required sample volumes.

A Microchip CE-MS Based End-to-End Solution for the Analysis of Synthetic Oligonucleotides

This poster showcases a workflow using microchip CE-MS that is coupled with a vendor-neutral informatics platform
as a complete solution for the analysis of synthetic oligos.

Microchip CE-MS with Integrated Solid Phase Extraction

Microchip CE-MS of Oligonucleotides: What Can It Separate?

Fast Chip-CEMS for Characterization of Protein/PTM Changes

Characterization of ADCs using ZipChip

Combined Top-down and Bottom-up Proteomics

ZipChip for Glycomics and Glycoproteomics

Fast and Efficient Biopharmaceutical Characterization

Characterization of charge heterogeneity of mAb

Rapid, Multi-level Analysis of Complex Proteins

Oligonucleotide Analysis via Microfluidic CE-MS

Advancing mAb Characterization with Microchip CE-MS Couples to a PASEF Enabled QTOF

96 Bottles of Beer: Metabolic Profiling of Spent Growth Media

Native Analysis of Monoclonal Antibodies

Characterization of Protein/PTM Changes

Native CE-MS for Biopharmaceutical Characterization

A Rapid Microfluidic Method for Molecular Weight Determination and Spent Media Analysis of an IgG1 Intact Protein in Growth Media

Bioprocessing of Monoclonal Antibody Based Therapies

NIBRT Presents Native MS for Charge Variants using ZipChip

NIBRT Presents ZipChip for Native Charge Heterogeneity

Monitoring Amino Acid Composition of Cell Culture Media

Published Articles

The Critical Role of CE-MS Methods for Biotherapeutic Characterization

Capillary electrophoresis (CE) methods play an important role in biomolecular characterization while microfluidic CE-mass spectrometry (MS) accelerates analytical workflows and improves development/commercialization timelines.

Identification of AAV capsid proteins

Characterization of a SRPK1/2 Inhibitor Complex

Detection and Quantification of neurotoxin β-N-methylamino-l-alanine

Characterizing Covalent CDK Inhibitor Complexes

Rapid Fingerprinting of a Highly Glycosylated Fusion Protein

Analysis of monosaccharides, oligosaccharides and glycopeptides

Metabolite Profiling Reveals Predictive Biomarkers

Monitoring Glycosylation Profile and Protein Titer in Cell Culture Samples

Characterizing Antiviral Protein-Inhibitor Complexes

Characterization of NIST mAb Proteoforms and Glycoforms

In-depth analysis of monoclonal antibodies using microfluidic capillary electrophoresis and native mass spectrometry

Comparative Elucidation of Cetuximab Heterogeneity on the Intact Protein Level

Characterization of Protein Assemblies Related to Cancer Proliferation

Analysis of alkaloids in plant cell cultures

Safety Data Sheet (SDS)

SDS – ZipChip Reagents: Oligos

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – Deoxyribonucleic Acid, Unmodified

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Standard: Amino Acids

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Interface Tray Grease

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Reagents: Native Antibodies

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Reagents: Charge Variant – TOF

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip BGE: Intact Antibodies

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Diluent: Intact Antibodies

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Acid: Metabolites

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip BGE: Metabolites

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Diluent: Metabolites

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip BGE: Peptides

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Diluent: Peptides

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

Webinars

ZipChip: A Versatile Platform (Webinar)

A Versatile Platform for Small Molecules, Intact Proteins & Beyond

ZipChip can be used for applications ranging from proteins and peptides in biopharmaceuticals, to oligonucleotides and AAVs in cell and gene therapy applications.

Simplifying Complex Glycoprotein Characterization Webinar

EPO Separation & Analysis

Straightforward separation and analysis of a highly glycosylated protein

Webinar: Simplify Oligo Characterization with Microchip CE-MS

Oligo Analysis Simplified: Get Your Answers Faster with Microchip CE-MS

Webinar: Simplify Oligo Characterization with Microchip CE-MS

Simplifying Oligonucleotide Characterization with Microchip CE-MS

In this on-demand webinar, we present a microfluidic capillary zone electrophoresis-based separation coupled with HRAM mass-spectrometry for rapid characterization of oligos in positive ionisation mode.

Analysis of inhibitor-protein complexes and small molecules

Fast Therapeutic Protein Characterization

In-depth characterization of complex monoclonal antibodies and antibody-drug conjugates with native ZipChip CE-MS

Streamline Protein Characterization and Profiling with CE-ESI-MS

Why use capillary electrophoresis

Simultaneous monitoring of amino acids and protein titer

Redefine Bioprocess Analytics

Zip Through the Backlog

ZipChip for Rapid and Easy Nanospray Infusions

Summary: Streamlined protein characterization and profiling

Rapid, comprehensive biotherapeutics characterization

NIBRT presents ZipChip for biopharma characterization

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