Application Notes & Snapshots

Quick screening of intact antibody and antibody drug conjugates with integrated microfluidic capillary electrophoresis and mass spectrometry

This application note shows a method for rapid screening of the heterogeneity of monoclonal antibodies and antibody-drug conjugates by an integrated microfluidic capillary electrophoresis (CE) and mass spectrometry (MS) workflow.

Rapid characterization of oligonucleotides using microfluidic CE-MS by ZipChip

This application note presents a CE-MS analysis of oligos by a ZipChip coupled to a QExactive HF mass spectrometer. This positive ESI mode method utilizes a MS-friendly background electrolyte (BGE), that eliminates the use of ionparing agents such as hexafluoro isopropanol or alkyl amines.

Charge variant analysis with ZipChip and maXisII

A ZipChip capillary zone electrophoresis system coupled to Bruker maXis II UHR-QTOF offers an alternative selectivity to separate charge variants prior to MS analysis and affords a more complete intact mass characterization without adding complexity or high salt eluents.

A ZipChip Based CZE-MS Analysis for Quick Assessment of Biotherapeutics

From comprehensive characterization to monitoring product degradation characteristics

Charge Variant Analysis Coupled to a SCIEX TripleTOF® 6600+ System Mass Spectrometer

High-Throughput Intact Mass Analysis of mAb Based Therapeutics

Application Snapshot Booklet Fermentation

Application Snapshot Booklet Cell and Gene Therapy

Application Snapshot Booklet Cell Culture

Application Snapshot Booklet Biologics

Application Snapshot Booklet

How comparable are different vendors’ LB media?

High variations of analyte levels in Terrific broth (TB) media

Investigating the high nutrient variations in Super Optimal Broth

Cultures’ growth rates and productivities may differ when different vendor’s media are used in otherwise identical experiments.

Probing formulation differences in Super Optimal Broth

Analysis of Reduced mAbs and ADCs

Similarities between different vendors’ 2X YT liquid media

Rapid Characterization of Intact mAb-based Biotherapeutics

Quick screening of intact antibody and ADC

Comparing standard microbial media from a single supplier

ZipChip for profiling and characterization of antibody charge heterogeneity

Sciex Flexible Solution for MAM with ZipChip

At-line spent media analysis is now a reality

To elevate process development decisions to real-time, researchers require new analytical platforms that simplify analysis and accelerate delivery of actionable intelligence.

Differences in fresh commercially available hybridoma media

Select the best CHO media from the start

Performing your own quick assays of the media you receive ensures consistency in the cell media fed to your cells.

Screening of chemically-defined media for suspension cultures of HEK293 cells

Across four vendors, there was a high variation in the fresh media composition.

Sciex Presents Charge Heterogeneity of Monoclonal Antibodies with ZipChip

Analyze media without removing your mAb on the Rebel

Identify variations in dry and liquid CHO media

Running a quick fresh media assay prior to feeding the culture ensures consistency of the media components when scaling up or during the transition between liquid-to-dry media blends.

Snapshot: Charge Variant Analysis

Charge Variant Analysis of Native Antibodies for In-depth Characterization of mAb-based Biotherapeutics

Serum supplemented media analysis is routine with the Rebel

Bioreactor Monitoring via ZipChip

Tease out variations in dry cell culture media

Running a simple media assay prior to feeding your cells can ensure that the composition of the media is what is expected.

Snapshot: High-Throughput Intact Mass

Snapshot: High Throughput Charge Variant Analysis

Snapshot: Cell Culture Analysis

Supplementing RPMI media with fetal bovine serum

Supplementing IMDM media with fetal bovine serum

Conventional and chemically-defined T cell medias

Amino acid contents of Sf9 and Sf21 insect cell media

Knowledge of the insect cell metabolism coupled with an understanding of the amino acid content of the cell media allows for a more informed process development analytics strategy.

Comparing mesenchymal stem cell media with supplements on the Rebel

To ensure consistency when culturing MSCs, researchers should understand what is being added to their media.

Don’t sweat your cell media additives

Handle non-ionic surfactants in cell media samples with ease

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Basal cell media with 10% fetal bovine serum

Posters

Bottom-Up Analysis of Progressively Larger RNAs by CZE-MS and CZE-MS/MS

In this poster from ASMS 2022, researchers from University of Connecticut and Bruker showed that capillary zone electrophoresis performed on a chip proved to be an effective alternative to liquid chromatography as a front-end separation system to analyze both pure samples and complex digestion mixtures of nucleic acids.

Multi-level Expeditious Characterization of Adeno-associated Virus (AAV) Capsid Proteins Using ZipChip Microfluidic CE Coupled with HRMS

In this poster from ASMS 2022, scientists from Thermo Fisher Scientific and 908 Devices present a novel approach using microchip CE-MS for the characterization of AAVs.

A Sensitive and High-Throughput Assay for Analyzing Peptide Exchanged MHCl by High-Resolution Native CZE-MS

In this poster from ASMS 2022, researchers from Genentech describe a sensitive and high-throughput assay using capillary-zone electrophoresis coupled to a high-resolution mass spectrometer for characterizing peptide-exchanged MHCI under native conditions.

Combining μSPE ZipChip CE with PRM-LIVE on a timsTOF Pro for Warp-speed Selectivity Profiling of Deubiquitinase (DUB) Small Molecule Inhibitors

In this ASMS 2022 poster, researchers from Dana Farber Cancer Institute and Harvard Medical School developed a ZipChip-based method for facilitating high-throughput identification
of new small molecule inhibitors.

Microchip CE-MS Analysis of Sialylated N-glycan Linkage Isomers

In this ASMS 2022 poster, Fudan University researchers show a ZipChip-based strategy for qualitative and quantitative analysis of sialylated N-glycan linkage isomers was developed.

CE-MS of Oligonucleotides using a ZipChip

This ASMS 2022 poster from Boehringer Ingelheim explores the use of ZipChip coupled to a high resolution mass spectrometer as an orthogonal method to chromatography for oligonucleotide analysis.

Glycosylation Profiling of mAB Subunits With CZE-MS

This ASMS 2022 poster from Bruker shows ZipChip coupled with high resolution mass spectrometry providing charge-based selectivity which can assist with glycoforms separation.

Rapid Characterization of Oligos and Related Impurities Using Microchip CE-MS Without Ion-pairing Reagents

This poster from ASMS 2022 describes a simple & fast method for oligonucleotide characterizations using a Microchip CE-MS platform.

Using CE-HPMS For Accelerated Process Development of a mAb-Expressing Cell Line

The coupling of miniature mass spectrometry (MS) to a microchip capillary electrophoresis (CE) platform provides a simple solution for at-line amino acid quantitation.

Pushing the Boundaries of Speed and Sensitivity in Proteomics

To overcome sample loading limitations in CE-MS, the poster authors engineered a 1 mm SPE bed within the chip geometry, allowing 100-fold improvements in concentration sensitivity limit for CE-MS.

A Broad Application of Migration Time Indexing and Metabolite Libraries to Evaluate Quantitative Coverage in Microchip CE-MS Metabolomics

In the field of metabolomics, microchip capillary electrophoresis (mCE) has a number of potential advantages over traditional liquid chromatography (LC), including higher throughput due to decreased separation times, increased separation efficiency, and lower required sample volumes.

Dynamic Range Optimization for Amino Acid Quantitation with an Integrated CE-MS Analyzer

The coupling of miniature mass spectrometry (MS) to a microchip capillary electrophoresis (CE) platform provides a simple solution for amino acid quantitation.

A Microchip CE-MS Based End-to-End Solution for the Analysis of Synthetic Oligonucleotides

This poster showcases a workflow using microchip CE-MS that is coupled with a vendor-neutral informatics platform
as a complete solution for the analysis of synthetic oligos.

Poster-BPI-West-2022

Rapid at line quantitation of key nutrients in cell culture media

This poster details the benefits of coupling of miniature mass spectrometry to a microchip capillary electrophoresis platform for characterizing cell media metabolites in fresh and spent media.

Microchip CE-MS with Integrated Solid Phase Extraction

Quantitation of metabolites in classical and customized cell culture media by an integrated CE-MS Analyzer

Quantitation of metabolites in cell culture media by an integrated CE-MS analyzer for upstream process development

Microchip CE-MS of Oligonucleotides: What Can It Separate?

Fast Chip-CEMS for Characterization of Protein/PTM Changes

Characterization of ADCs using ZipChip

Combined Top-down and Bottom-up Proteomics

ZipChip for Glycomics and Glycoproteomics

Fast and Efficient Biopharmaceutical Characterization

Characterization of charge heterogeneity of mAb

Rapid, Multi-level Analysis of Complex Proteins

Oligonucleotide Analysis via Microfluidic CE-MS

Advancing mAb Characterization with Microchip CE-MS Couples to a PASEF Enabled QTOF

96 Bottles of Beer: Metabolic Profiling of Spent Growth Media

Using genomic and spent media analysis to characterize stressed CHO cultures

Native Analysis of Monoclonal Antibodies

Characterization of Protein/PTM Changes

Native CE-MS for Biopharmaceutical Characterization

A Rapid Microfluidic Method for Molecular Weight Determination and Spent Media Analysis of an IgG1 Intact Protein in Growth Media

Characterizing the Analytical Performance and Reliability of Metabolite Quantitation by an Integrated CE-MS Analyzer

Rapid Analysis of Cell Therapy Media

Quantitation of CHO media nutrients and metabolites in under 180 seconds

Rapid analysis of amino acids, water soluble vitamins, biogenic amines and dipeptides in cell growth media

Rapid at line nutrient profiling from an ammonia stressed CHO cell line

Bioprocessing of Monoclonal Antibody Based Therapies

NIBRT Presents Native MS for Charge Variants using ZipChip

NIBRT Presents ZipChip for Native Charge Heterogeneity

Monitoring Amino Acid Composition of Cell Culture Media

Published Articles

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In-Process Analytics are Key to Successful Cell Therapy Process Optimization and Manufacturing

Considerable clinical activity and the approval of several autologous, patient-specific therapies has increased industry demand and has also highlighted bottlenecks in manufacturing workflows.

The Critical Role of CE-MS Methods for Biotherapeutic Characterization

Capillary electrophoresis (CE) methods play an important role in biomolecular characterization while microfluidic CE-mass spectrometry (MS) accelerates analytical workflows and improves development/commercialization timelines.

Identification of AAV capsid proteins

Characterization of a SRPK1/2 Inhibitor Complex

Detection and Quantification of neurotoxin β-N-methylamino-l-alanine

Characterizing Covalent CDK Inhibitor Complexes

Rapid Fingerprinting of a Highly Glycosylated Fusion Protein

Analysis of monosaccharides, oligosaccharides and glycopeptides

Metabolite Profiling Reveals Predictive Biomarkers

Monitoring Glycosylation Profile and Protein Titer in Cell Culture Samples

Characterizing Antiviral Protein-Inhibitor Complexes

Characterization of NIST mAb Proteoforms and Glycoforms

Spent Media Analysis of CHO Grown in an Ammonia-Stressed Parallel Microbioreactor Platform

In-depth analysis of monoclonal antibodies using microfluidic capillary electrophoresis and native mass spectrometry

Comparative Elucidation of Cetuximab Heterogeneity on the Intact Protein Level

Characterization of Protein Assemblies Related to Cancer Proliferation

Analysis of alkaloids in plant cell cultures

Safety Data Sheet (SDS)

SDS – ZipChip Reagents: Oligos

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – Deoxyribonucleic Acid, Unmodified

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Standard: Amino Acids

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Interface Tray Grease

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Reagents: Native Antibodies

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Reagents: Charge Variant – TOF

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip BGE: Intact Antibodies

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Diluent: Intact Antibodies

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Acid: Metabolites

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip BGE: Metabolites

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Diluent: Metabolites

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip BGE: Peptides

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

SDS – ZipChip Diluent: Peptides

Download this Safety Data Sheet (SDS) for documentation related to the properties of each chemical in the product; the physical, health, and environmental health hazards; protective measures; and safety precautions for handling, storing, and transporting the product.

Webinars

The Critical Role of Cell Culture Media Analysis in CGT Development

Does cell culture analysis matter for CGT development? This short webinar explores what is already known, reviews at-line methods for cell culture media analysis, and shares recent data from two case studies.

Biopharma 4.0: Finding Opportunities to Leverage New At-Line Data

Listen how REBEL users describe how they use better data acquisition, management platforms, and process modeling to implement process intensification.

Process Modeling with Researchers from Johns Hopkins University

Listen to a dynamic discussion on the highlights of a new peer-reviewed article exploring model-based approached to cell culture optimization

Webinar: EPO Separation and Analysis

EPO Separation & Analysis

Straightforward separation and analysis of a highly glycosylated protein

Oligo Analysis Simplified: Get Your Answers Faster with Microchip CE-MS

Ask the Expert: Bioprocess 101 – Cell Culture Media Analysis

Process Intensification: Do You Have All the Data You Need When You Need It?

The need for process intensification and implementation of Bioprocess 4.0 requires extensive real time data acquisition and process modelling. At line MS-based technologies presented here provide key cellular metabolic and protein quality data in real time to support process intensification and modelling.

Simplifying Oligonucleotide Characterization with Microchip CE-MS

In this on-demand webinar, we present a microfluidic capillary zone electrophoresis-based separation coupled with HRAM mass-spectrometry for rapid characterization of oligos in positive ionisation mode.

Analysis of inhibitor-protein complexes and small molecules

Fast Therapeutic Protein Characterization

In-depth characterization of complex monoclonal antibodies and antibody-drug conjugates with native ZipChip CE-MS

Streamline Protein Characterization and Profiling with CE-ESI-MS

The Importance of Cell Media Analysis in Upstream Bioprocess Development

Mass Spectrometry-Based Process Analytical Technologies for Cell Therapies

Why use capillary electrophoresis

Unlocking rapid cell culture media analysis for cell and gene therapy process development

Be a REBEL with Your Cell Media Analytics

Simultaneous monitoring of amino acids and protein titer

Redefine Bioprocess Analytics

Zip Through the Backlog

ZipChip for Rapid and Easy Nanospray Infusions

Summary: Streamlined protein characterization and profiling

Rapid, comprehensive biotherapeutics characterization

Rapid at-line amino acid and nutrient profiling from bioprocess media with the REBEL

How can we accelerate PD analytics?

NIBRT presents ZipChip for biopharma characterization

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