Application Note

ZipChip App Note 8.5

A ZipChip Based CZE-MS Analysis for Quick Assessment of Biotherapeutics

Native charge variant analysis of biotherapeutics using ZipChip CE-ESI-MS: From comprehensive characterization to monitoring product degradation characteristics

Charge Variant Analysis Coupled to a SCIEX TripleTOF® 6600+ System Mass Spectrometer

High-Throughput Intact Mass Analysis of mAb Based Therapeutics

Analysis of Reduced mAbs and ADCs

Rapid Characterization of Intact mAb-based Biotherapeutics

Quick screening of intact antibody and ADC

ZipChip for profiling and characterization of antibody charge heterogeneity

Sciex Flexible Solution for MAM with ZipChip

Sciex Presents Charge Heterogeneity of Monoclonal Antibodies with ZipChip

Snapshot: Charge Variant Analysis

Charge Variant Analysis of Native Antibodies for In-depth Characterization of mAb-based Biotherapeutics

Bioreactor Monitoring via ZipChip

Snapshot: High-Throughput Intact Mass

Snapshot: High Throughput Charge Variant Analysis

Snapshot: Cell Culture Analysis

Application Snapshot

Comparing standard microbial media from a single supplier

Application Snapshot Booklet Fermentation

Application Snapshot Booklet Cell and Gene Therapy

Application Snapshot Booklet Biologics

How comparable are different vendors’ LB media?

High variations of analyte levels in Terrific broth (TB) media

Investigating the high nutrient variations in Super Optimal Broth

Cultures’ growth rates and productivities may differ when different vendor’s media are used in otherwise identical experiments.

Probing formulation differences in Super Optimal Broth

Similarities between different vendors’ 2X YT liquid media

Comparing standard microbial media from a single supplier

Be fast and accurate with at-line cell media analysis

At-line spent media analysis is now a reality

To elevate process development decisions to real-time, researchers require new analytical platforms that simplify analysis and accelerate delivery of actionable intelligence.

Differences in fresh commercially available hybridoma media

Select the best CHO media from the start

Performing your own quick assays of the media you receive ensures consistency in the cell media fed to your cells.

Screening of chemically-defined media for suspension cultures of HEK293 cells

Across four vendors, there was a high variation in the fresh media composition.

Analyze media without removing your mAb on the Rebel

Identify variations in dry and liquid CHO media

Running a quick fresh media assay prior to feeding the culture ensures consistency of the media components when scaling up or during the transition between liquid-to-dry media blends.

Serum supplemented media analysis is routine with the Rebel

Accelerate process development

Tease out variations in dry cell culture media

Running a simple media assay prior to feeding your cells can ensure that the composition of the media is what is expected.

Are you getting the cell culture media you expected?

Consistency between vendors should not be assumed.

Simply monitor initial vitamin levels

Supplementing RPMI media with fetal bovine serum

Supplementing IMDM media with fetal bovine serum

Conventional and chemically-defined T cell medias

Amino acid contents of Sf9 and Sf21 insect cell media

Knowledge of the insect cell metabolism coupled with an understanding of the amino acid content of the cell media allows for a more informed process development analytics strategy.

Comparing mesenchymal stem cell media with supplements on the Rebel

To ensure consistency when culturing MSCs, researchers should understand what is being added to their media.

Don’t sweat your cell media additives

Handle non-ionic surfactants in cell media samples with ease

Basal cell media with 10% fetal bovine serum

Poster

Fast Chip-CEMS for Characterization of Protein/PTM Changes

Characterization of ADCs using ZipChip

Combined Top-down and Bottom-up Proteomics

ZipChip for Glycomics and Glycoproteomics

Fast and Efficient Biopharmaceutical Characterization

Characterization of charge heterogeneity of mAb

Rapid, Multi-level Analysis of Complex Proteins

Oligonucleotide Analysis via Microfluidic CE-MS

Advancing mAb Characterization with Microchip CE-MS Couples to a PASEF Enabled QTOF

96 Bottles of Beer: Metabolic Profiling of Spent Growth Media

Using genomic and spent media analysis to characterize stressed CHO cultures

Native Analysis of Monoclonal Antibodies

Characterization of Protein/PTM Changes

Native CE-MS for Biopharmaceutical Characterization

A Rapid Microfluidic Method for Molecular Weight Determination and Spent Media Analysis of an IgG1 Intact Protein in Growth Media

Characterizing the Analytical Performance and Reliability of Metabolite Quantitation by an Integrated CE-MS Analyzer

Rapid Analysis of Cell Therapy Media

Rapid analysis of amino acids, water soluble vitamins, biogenic amines and dipeptides in cell growth media

Rapid at line nutrient profiling from an ammonia stressed CHO cell line

Bioprocessing of Monoclonal Antibody Based Therapies

NIBRT Presents Native MS for Charge Variants using ZipChip

NIBRT Presents ZipChip for Native Charge Heterogeneity

Monitoring Amino Acid Composition of Cell Culture Media

Published Article

Webinar

Analysis of inhibitor-protein complexes and small molecules

Fast Therapeutic Protein Characterization

In-depth characterization of complex monoclonal antibodies and antibody-drug conjugates with native ZipChip CE-MS

Streamline Protein Characterization and Profiling with CE-ESI-MS

The Importance of Cell Media Analysis in Upstream Bioprocess Development

Mass Spectrometry-Based Process Analytical Technologies for Cell Therapies

Why use capillary electrophoresis

Unlocking rapid cell culture media analysis for cell and gene therapy process development

Be a REBEL with Your Cell Media Analytics

Simultaneous monitoring of amino acids and protein titer

Redefine Bioprocess Analytics

Zip Through the Backlog

ZipChip for Rapid and Easy Nanospray Infusions

Summary: Streamlined protein characterization and profiling

Rapid, comprehensive biotherapeutics characterization

Rapid at-line amino acid and nutrient profiling from bioprocess media with the Rebel

How can we accelerate PD analytics?

NIBRT presents ZipChip for biopharma characterization

email Subscribe to Our Newsletter Signup to receive new product updates, technical tips and more.