Empowering Traditional Mass Spec

ZipChip™ uses integrated microfluidic technology to prepare, separate and electrospray biological samples directly into traditional mass spectrometers (MS). In less than 3 minutes per sample, the cost-effective ZipChip system enables analysis of a broad range of matrices from growth media to cell lysates, blood, plasma, urine, and biopharma products. The chips provide answers on analytes from small molecules up to intact proteins, antibodies and antibody drug conjugates (ADC). ZipChip provides better separation quality than most liquid chromatography (LC) instruments – in a fraction of the time – all with full MS identification behind every separations peak.   The ZipChip interface simply mounts onto your mass spectrometer for seamless integration with existing data collection and processing.

New Technology For Speedy Answers

ZipChip uses cutting-edge microfluidic technology to integrate capillary electrophoresis (CE) and electrospray ionization (ESI) on a chip about the size of a microscope slide. This eliminates junctions between individual components and removes dead volumes that slow and degrade analytical quality in conventional separation techniques. Pick ZipChip HS for small molecules or ZipChip HR for intact proteins – and off you go.

A Chip With Smarts

Each individual ZipChip has intelligence built in. The system recognizes your ZipChip as soon as it is loaded and will even automatically load run parameters and custom methods based on your preferences. Performance and total runs completed are tracked to ensure reliable results. When used with ZipChip Assay Kits, we’ll even guarantee the lifetime of each chip.

Eliminate The Guesswork

Our premixed background electrolyte consumables further reduce preparation time and minimize opportunities for human error when diluting and analyzing samples. These customized ZipChip Assay Kits are manufactured under our ISO-9001 quality system and optimized for your small and intact large molecule sample types.

One Step Prep

A single pre-programmed method works across sample types, typically with electrolyte dilution, as your only step in sample prep. Desalting is automatically taken care of. ZipChip saves time and ensures answers are derived from samples in their native states.

Automated For Productivity

Combining ZipChip with the ZipChip Autosampler generates dramatic productivity improvements. Users achieve unattended sample runs from sample vials or 96-well plates.   When complete simply change the chip and run another 96.

We Value Your Precious Samples

ZipChip needs only a few microliters of sample for analysis, which is great when your development samples are precious. When your lab has high demand, the low-volume and friendly background electrolyte provides analysis that is gentle on your mass spectrometer. ZipChip provides minimum downtime and maximum quantitative stability over time.

ZipChip

ADCs
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ADCs

Characterizing antibody drug conjugates, or ADCs, is critical and fraught with challenges due to their inherent complexity. Antibodies exist in complex populations due to posttranslational modifications and degradation during expression, processing and storage. ADCs have an additional layer of complexity due to differences in their drug-to-antibody ratio (DAR) brought about by the conjugation process.

Amino Acids & Metabolites
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Amino Acids & Metabolites

The detection and relative quantification of metabolites is key to understanding the basic functionality of living systems. Spanning fundamental discovery, drug development, research into health and nutrition, and biomarkers for disease states, Mass Spectrometry is the key tool of the modern life scientist. With ZipChip as a front-end liquid separation and electrospray ionization source, users dramatically increase throughput and metabolite analysis capabilities of their Mass Spectrometer.

Cell Lysate
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Cell Lysate

Analysis of cells is fundamental to the the understanding of functioning biological systems from discovery of oncology drugs, to DMPK and toxicology studies for all pharmaceuticals. Cells contain thousands of compounds in constant metabolomic flux so capturing an accurate ‘snap-shot,’ requires fast analysis and minimal sample preparation.

Growth Media
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Growth Media

At the heart of the biotech and biopharma revolutions is the fermentation process and growth media is the fuel. Optimizing culture conditions is key to production of proteins with minimal structural modification and highest yield. Monitoring the consumption of amino acids and key nutrients from the media and the production of key metabolites is not only key in research and scale-up but good practice in batch manufacturing and critical in continual flow reactors.

Intact Proteins & Antibodies
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Intact Proteins & Antibodies

Intact proteins and antibodies, whether naturally occurring or manufactured, exist as a population of variants. These complex populations are the result of post translational modifications and degradation that can occur during expression, processing and storage. These modifications may include disulfide bond scrambling, glycosylation, deamidation, pyroglutamate formation, C-terminal lysine truncation, oxidation, and amino acid substitutions among others.

Peptide Mapping
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Peptide Mapping

Analysis of proteins by digestion and peptide mapping is typically run with Liquid Chromatography Mass Spectrometry (LC-MS). There are limitations: Chromatography is both slow, often 30 minutes or more per sample, and depends on retention of analytes on a stationary phase – incompatible with some sample types. Small, polar peptides typically aren’t retained while large, ‘sticky’ peptides get trapped on column and don’t elute for analysis.

ZipChip Specifications

Zip Chip HS Zip Chip HR
Separation channel length /cm 10  22
On Chip De-salting  Yes  Yes
Integrated ESI  Yes  Yes
Metabolite and Small Molecule Analysis
Recommended use Normal high resolution analysis Ultimate resolving power required
Analysis time (typical) ~ 2 minutes ~ 9 minutes
Peptide Mapping
Recommended use Normal high resolution analysis Ultimate resolving power required
Analysis time ~ 3 minutes ~ 12 minutes
Intact Protein Analysis
Recommended use Fast injection, relatively pure samples Normal high resolution analysis
Analysis time < 1min  ~3 minutes

Interface Specifications

Mounting Configuration All Thermo Fisher Scientific Exactive, Qexactive Orbitrap,and LTQ-Orbitrap MS instruments. Also compatible with Thermo ion trap MS and select triple quadrupole MS instruments.
Operation
  • Fully automated
  • Push button start
Software
  • ZipChip software for configuration of injection parameters
  • Integration with Thermo Fisher Scientific Xcalibur software for data collection, processing and reporting
Dimensions 8in (h) x 8in (w) x 11in (d)
Weight 7lbs
Power Requirements 110/240V
Auto Sampler Integrated 96-well format sample plates
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