Unique Intact Proteins Insight

Intact proteins and antibodies, whether naturally occurring or manufactured, exist as a population of variants. These complex populations are the result of post translational modifications and degradation that can occur during expression, processing and storage. These modifications may include disulfide bond scrambling, glycosylation, deamidation, pyroglutamate formation, C-terminal lysine truncation, oxidation, and amino acid substitutions among others. In order to be used as a therapeutic agent the biomolecule must be fully characterized to ensure safety, efficacy and potency. Mass Spectrometry is ideal for unique identification but requires pre-separation to simplify the data for analysis. Historical separation techniques for proteins and antibodies are incompatible with MS as they require high salt content.

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Full Characterization. Fast.

ZipChip is the only directly MS-compatible technique that separates protein and antibody variants. ZipChips integrate both separation and electrospray ionization on a single device – near native-state protein variants with a full mass spectrum behind every peak! With ZipChip HR, proteins require no sample prep and analysis is complete in 3 minutes.

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AAPS National Biotechnology Conference, San Diego, CA

05.01.17 - 05.03.17

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