At the heart of the biotech and biopharma revolutions is the fermentation process and growth media is the fuel. Optimizing culture conditions is key to production of proteins with minimal structural modification and highest yield. Monitoring the consumption of amino acids and key nutrients from the media and the production of key metabolites is not only key in research and scale-up but good practice in batch manufacturing and critical in continual flow reactors. Complexity and expense mean many groups monitor media only after completion but the speed and simplicity of ZipChip enables frequent, in-process monitoring.
Using ZipChip HS with your Mass Spectrometer for growth media analysis gives you full Amino Acid profiles and quantification of key metabolites in less than 3 minutes. Analysis involves minimal sample preparation while low flow rates and a volatile background electrolyte are gentle on your MS system – maximum uptime, maximum analytical integrity.
ZipChip™ uses integrated microfluidic technology to prepare, separate and electrospray biological samples directly into traditional mass spectrometers (MS).
In less than 3 minutes per sample, the cost-effective ZipChip analyzes a broad range of matrices from growth media to cell lysates, blood, plasma, urine and biopharma products. ZipChip delivers answers on analytes from small molecules and peptides up to intact proteins, antibodies and antibody drug conjugates; providing better separation quality than most liquid chromatography instruments — in a fraction of the time — all with full MS identification behind every separations peak. The ZipChip interface simply mounts onto your mass spectrometer for seamless integration with existing data collection and processing.