Characterizing antibody drug conjugates, or ADCs, is critical and fraught with challenges due to their inherent complexity. Antibodies exist in complex populations due to posttranslational modifications and degradation during expression, processing and storage. ADCs have an additional layer of complexity due to differences in their drug-to-antibody ratio (DAR) brought about by the conjugation process. Different pharmacokinetic properties associated with each variant typically make the DAR and distribution critical quality attributes in manufacturing. Mass Spectrometry is ideal for unique identification of variants, but requires pre-separation to simplify the data for analysis. Historical ADC separation techniques are incompatible with MS as they require high salt content.View Resources
ZipChip is ideal for ADC analysis and gives favorable results to imaging CE (iCE). ZipChips integrate both separation and electrospray ionization into your mass spec – near-native intact ADC variants with a full mass spectrum behind every peak! With ZipChip HR, ADCs require no sample prep and analysis is complete in 3 minutes.
05.01.17 - 05.03.17